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Open AccessArticle

Vitamin D Regulates Maternal T-Helper Cytokine Production in Infertile Women

Department of Obstetrics and Gynecology, Juntendo University, Faculty of Medicine, Tokyo 1138421, Japan
Center for Reproductive Medicine and Implantation Research, Sugiyama Clinic Shinjuku, Tokyo 1160023, Japan
Author to whom correspondence should be addressed.
Nutrients 2018, 10(7), 902;
Received: 12 June 2018 / Revised: 2 July 2018 / Accepted: 10 July 2018 / Published: 13 July 2018
Vitamin D (VD) deficiency is associated with reproductive failure. However, the relationship between VD and maternal immunity remains unclear. We investigated the clinical efficacy of VD in maternal T-helper (Th) cytokines in 276 infertile women and examined for Th1 and Th2 cells based on the deficient, insufficient, and sufficient serum 25-hydroxyvitamin D3 (25[OH]VD) levels (<12, 12–30, and >30 ng/mL, respectively). Most infertile women had a low-level of VD (87.3%). Immunological tests of pre-/post-VD supplementation were performed in patients who were deficient and insufficient in VD. Of 23 patients, 11 (47.8%) exhibited sufficient VD levels after supplementation. Th1/Th2 cell ratio in patients with insufficient VD was significantly decreased after supplementation (p = 0.004). After supplementation, serum 25(OH)VD levels of the patients: 11 in the sufficient group showed significant decreases in Th1 cell level and Th1/Th2 cell ratio (p = 0.032 and 0.010, respectively), whereas no significant differences in Th1/Th2 cell ratio were recognized in the insufficient group. Furthermore, mid-luteal endometrial biopsies (n = 18) were processed for primary cultures and measured interferon [IFN]-γ and interleukin [IL]-4 in condition media. Decidualizing cultures with 1,25-dihydroxvitamin D3 (1,25[OH]2VD) decreased IFN-γ. Sufficient VD supplementation in women with insufficient VD may optimize maternal T-helper cytokines during pregnancy via rebalancing the Th1/Th2 cell ratio. View Full-Text
Keywords: vitamin D; preconception care; helper T cell; infertility vitamin D; preconception care; helper T cell; infertility
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Ikemoto, Y.; Kuroda, K.; Nakagawa, K.; Ochiai, A.; Ozaki, R.; Murakami, K.; Jinushi, M.; Matsumoto, A.; Sugiyama, R.; Takeda, S. Vitamin D Regulates Maternal T-Helper Cytokine Production in Infertile Women. Nutrients 2018, 10, 902.

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