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Open AccessArticle

Beneficial Effect of Jojoba Seed Extracts on Hyperglycemia-Induced Oxidative Stress in RINm5f Beta Cells

1
UMR DIATHEC, EA 7294, Fédération de Médecine Translationnelle de Strasbourg (FMTS), University of Strasbourg, Boulevard René Leriche, 67200 Strasbourg, France
2
Institut Supérieur de Biotechnologie de Sfax (ISBS), Road of Soukra Km 4, PO Box, Sfax 1175, Tunisia
3
Laboratory of Bioactive Substances, Center of Biotechnology of Borj Cédria, BP 901, Hammam-lif 2050, Tunisia
4
AGRO-CRC, Al Amine Al Abbassi Street, Tunis 1002, Tunisia
*
Author to whom correspondence should be addressed.
Nutrients 2018, 10(3), 384; https://doi.org/10.3390/nu10030384
Received: 18 January 2018 / Revised: 12 March 2018 / Accepted: 15 March 2018 / Published: 20 March 2018
Hyperglycemia occurs during diabetes and insulin resistance. It causes oxidative stress by increasing reactive oxygen species (ROS) levels, leading to cellular damage. Polyphenols play a central role in defense against oxidative stress. In our study, we investigated the antioxidant properties of simmondsin, a pure molecule present in jojoba seeds, and of the aqueous extract of jojoba seeds on fructose-induced oxidative stress in RINm5f beta cells. The exposure of RINm5f beta cells to fructose triggered the loss of cell viability (−48%, p < 0.001) and disruption of insulin secretion (p < 0.001) associated with of reactive oxygen species (ROS) production and a modulation of pro-oxidant and antioxidant signaling pathway. Cell pre-treatments with extracts considerably increased cell viability (+86% p < 0.001) for simmondsin and +74% (p < 0.001) for aqueous extract and insulin secretion. The extracts also markedly decreased ROS (−69% (p < 0.001) for simmondsin and −59% (p < 0.001) for aqueous extract) and caspase-3 activation and improved antioxidant defense, inhibiting p22phox and increasing nuclear factor (erythroid-derived 2)-like 2 (Nrf2) levels (+70%, p < 0.001) for aqueous extract. Simmondsin had no impact on Nrf2 levels. The richness and diversity of molecules present in jojoba seed extract makes jojoba a powerful agent to prevent the destruction of RINm5f beta cells induced by hyperglycemia. View Full-Text
Keywords: RINm5f; oxidative stress; jojoba; caspase-3; Nrf2; p22phox RINm5f; oxidative stress; jojoba; caspase-3; Nrf2; p22phox
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Belhadj, S.; Hentati, O.; Hamdaoui, G.; Fakhreddine, K.; Maillard, E.; Dal, S.; Sigrist, S. Beneficial Effect of Jojoba Seed Extracts on Hyperglycemia-Induced Oxidative Stress in RINm5f Beta Cells. Nutrients 2018, 10, 384.

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