The Protective Effect of Polygonum cuspidatum (PCE) Aqueous Extract in a Dry Eye Model
Abstract
:1. Introduction
2. Materials and Methods
2.1. Preparation of Polygonum Cuspidatum Extract
2.2. Animals and Treatment
2.3. Tear Volume Measurement
2.4. Analysis of Corneal Irregularity
2.5. Immunohistochemistry (IHC)
2.6. Human Corneal Epithelial Cell (HCEC) Culture
2.7. Cell Viability Assay
2.8. Western Blot Analysis
2.9. Nuclear and Cytosol Protein Extraction
2.10. Quantitative Realtime-Polymerase Chain Reaction (PCR)
2.11. Statistical Analysis
3. Results
3.1. Polygonum Cuspidatum Active Compound Isolation and Determination
3.2. Effects of PCE on Tear Production
3.3. Effects of PCE on Corneal Surface Irregularities
3.4. Effects of PCE on MUC4 Expression and Inflammatory Cytokines
3.5. Effects of PCE on Hyperosmolar Stress-Induced Cell Death
3.6. Effects of PCE on Inflammation
3.7. Effects of PCE on Hyperosmolar Stress-Induced Apoptotic Cell Death
3.8. Effects of PCE on Hyperosmolar Stress-Induced Oxidative Stress
4. Discussion
5. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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Genes | Sequence | |
---|---|---|
rMuc4 | F | 5′-CGGATTCCTTCTACGTTACAG-3′ |
R | 5′-GAATCGATCTGGACGGTACTTC-3′ | |
rMuc4 | F | 5′-AGAGACTTCCAGCCAGTTGC-3′ |
R | 5′-AGCCTCCGACTTGTGAAGTG-3′ | |
rMuc4 | F | 5′-TCGTCTACTCCTCAGAGCCC-3′ |
R | 5′-ACTTCAGCGTCTCGTGTGTT-3′ | |
rMMP9 | F | 5′-CGCTTGGATAACGAGTTCTCTC-3′ |
R | 5′-GCAGGAGGTCATAGGTCACG-3′ | |
rGAPDH | F | 5′-CGTGAAAAGATGACCCAGAT-3′ |
R | 5′-ACCCTCATAGATGGGCACA-3′ | |
hMuc4 | F | 5′-GCCCAAGCTACAGTGTGACTCA-3′ |
R | 5′-ATGGTGCCGTTGTAATTTGTTGT-3′ | |
hIL-6 | F | 5′-AAATTCGGTACATCCTCGAC-3′ |
R | 5′-CAGGAACTGGATCAGGACTT-3′ | |
hTNF-α | F | 5′-TTCTCCTTCCTGCTTGTG-3′ |
R | 5′-CTGAGTGTGAGTGTCTGG-3′ | |
hMMP9 | F | 5′-GGGACGCAGACATCGTCATC-3′ |
R | 5′-TCGTCATCGTCGAAATGGGC-3′ | |
hGAPDH | F | 5′-CCAGCCGAGCCACATCGCTC-3′' |
R | 5′-ATGAGCCCCAGCCTTCTCCAT-3′ |
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Park, B.; Lee, I.S.; Hyun, S.-W.; Jo, K.; Lee, T.G.; Kim, J.S.; Kim, C.-S. The Protective Effect of Polygonum cuspidatum (PCE) Aqueous Extract in a Dry Eye Model. Nutrients 2018, 10, 1550. https://doi.org/10.3390/nu10101550
Park B, Lee IS, Hyun S-W, Jo K, Lee TG, Kim JS, Kim C-S. The Protective Effect of Polygonum cuspidatum (PCE) Aqueous Extract in a Dry Eye Model. Nutrients. 2018; 10(10):1550. https://doi.org/10.3390/nu10101550
Chicago/Turabian StylePark, Bongkyun, Ik Soo Lee, Soo-Wang Hyun, Kyuhyung Jo, Tae Gu Lee, Jin Sook Kim, and Chan-Sik Kim. 2018. "The Protective Effect of Polygonum cuspidatum (PCE) Aqueous Extract in a Dry Eye Model" Nutrients 10, no. 10: 1550. https://doi.org/10.3390/nu10101550