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Sustainability 2017, 9(3), 454; https://doi.org/10.3390/su9030454

Article
Comparative Physicochemical and Microbiological Qualities of Source and Stored Household Waters in Some Selected Communities in Southwestern Nigeria
1
Department of Microbiology, Obafemi Awolowo University, Ile-Ife 220282, Nigeria
2
Department of Chemical Science, Yaba College of Technology, Lagos 101212, Nigeria
3
SAMRC Microbial Water Quality Monitoring Centre, University of Fort Hare, Alice 5700, South Africa
*
Author to whom correspondence should be addressed.
Academic Editor: Vincenzo Torretta
Received: 31 December 2016 / Accepted: 15 March 2017 / Published: 19 March 2017

Abstract

:
In this study, we evaluated the physicochemical and microbial qualities of source and stored household waters in some communities in Southwestern Nigeria using standard methods. Compared parameters include: physicochemical constituents; Temperature (T), pH, Total Dissolved Solids (TDS), Total Hardness (TH), Biological Oxygen Demand (BOD), Magnesium ion (Mg2+) and Calcium ion (Ca2+) and microbiological parameters included Total Coliform Counts (TC), Faecal Coliform Counts (FC), Fungal Counts (Fung C), Heterotrophic Plate Counts (HPC).Comparing Stored and Source samples, the mean values of some physicochemical parameters of most of the stored water samples significantly (p < 0.05) exceeded that of Sources and ranged in the following order: T (15.3 ± 0.3 °C–28.3 ± 0.5 °C), pH (6.4 ± 0.1–7.6 ± 0.1), TDS (192.1 ± 11.1 ppm–473.7 ± 27.9 ppm), TH (10.6 ± 1.7 mg/L–248.6 ± 18.6 mg/L), BOD (0.5 ± 0.0 mg/L–3.2 ± 0.3 mg/L), Mg2+ (6.5 ± 2.4 mg/L–29.1 ± 3.2 mg/L) and Ca2+ (6.5 ± 2.4 mg/L–51.6 ± 4.4 mg/L). The mean microbial counts obtained from microbial comparison of different points (Stored and Source) of collection showed that most of the stored water had counts significantly exceeding (p < 0.05) those of the source water samples (cfu/100 mL) which ranged as follows: TC (3.1 ± 1.5–156.8 ± 42.9), FC (0.0 ± 0.0–64.3 ± 14.2) and HPC (47.8 ± 12.1–266.1 ± 12.2) across all sampled communities. Also, the predominant isolates recovered from the samples were identified as Escherichia coli, Klebsiella pneumonia, Pseudomonas aeruginosa, Enterobacter aerogenes, Aspergillus spp., Mucor spp., Rhizopus spp. and Candida spp. The presence of these pathogenic and potentially pathogenic organisms in the waters and the high counts of the indicator organisms suggest the waters to be a threat to public health.
Keywords:
water; physical; chemical; microbiological; quality; household; stored; source

1. Introduction

Increase in development has brought about continuous scarcity of water resources in many parts of the world [1]. In Nigeria, access to safe water and sanitation is a major challenge, 53% of the populace in rural and 28% in urban areas have no access to improved water sources [2]. Water Aids Nigeria reported that around 57 million Nigerians lack access to safe potable water while over 130 million people (two thirds of the population) do not have access to adequate sanitation [3]. Water provides essential elements, but when polluted it may become undesirable substance that is dangerous to human health [4]. Water pollution is a main global problem, a leading cause of death and diseases which calls for evaluation and revision of water resources at all levels [5]. The specific contaminants leading to pollution in water include a wide spectrum of chemical, pathogens, physical or sensory changes such as elevated temperature and discoloration [6,7]. The pathogens include Salmonella species, Escherichia coli, parasitic worm, virus (hepatitis A), helminthes such as guinea worm [8,9].
Lack of safe drinking water and inadequate sanitation measures introduce diseases causing pathogens such as E. coli, Salmonella species, Vibrio cholera into water. These pathogens can cause water-borne diseases like cholera, typhoid, nausea, cramp and diarrhoea in either human or animal hosts [10]. Water-borne pathogens pose special risk for millions of lives especially infants, young children under the age of five and people with severe compromised immune system [11,12]. Every year millions of lives are claimed in developing countries and death of more than 2 million people per year worldwide is caused by diarrhoea, mostly among children under the age of five [11,12]. The purity of water depends on its source, treatment received and storage facilities available [13].
Surface and ground waters serve as sources of water for many people; however, these can be contaminated by biological and chemical pollutants arising from point and non-point sources [14]. Farmlands, urban residential subsistence and livestock farming have been shown as some of the effect of human activities on surface water quality [15]. The variations in the water quality were characterized by physicochemical parameters such as NH4-N, total N, soluble reactive phosphorus, total P NO3-N, temperature, pH and dissolved organic carbon. Hence, anthropogenic pollution influences physical and chemical parameters of water which in turn impact on the distribution and species diversity of biotic life in water bodies [16,17]. Also, surface water such as streams, rivers and lakes, which are sources of drinking water, are mostly untreated and associated with various health risks [18,19]. The groundwater is believed to be comparatively much clean and free from pollution than surface water but over exploitation of resources, prolonged discharge of industrial effluents, domestic sewage and solid waste dump causes the groundwater to become polluted and created health problems [20]. Other contaminants find their way into ground through activities of seepage of municipal landfills, and septic tank effluent. Likewise, indiscriminate waste disposal which are becoming serious in many Nigerian cities that lack efficient waste disposal system or treatment plants also contribute to contamination [21]. Availability of water through surface and groundwater resources is becoming critical day to day.
Only 1% part is available on land for drinking, agriculture, domestic power generation, industrial consummation, transportation and waste disposal [22]. Scarcity in quantity and access to water make storage of water imperative. Domestic storage of water can be made in a cemented reservoir, plastic tanks, bucket or metal tanks, earthen pot [23]. Storage is generally believed to reduce the number of microorganisms in water, nevertheless, several other factors affect microflora of stored water which include sedimentation, activities of other organisms, light ray, temperature and food supply [24].
Furthermore, uncovered containers are exposed to environmental conditions such as dust and dirt which may contribute to the deterioration in water quality [25,26,27]. Storage containers placed on the floor may be more likely contaminated by animals or children than containers placed on an elevated surface [28,29]. Water stored in open-top containers appears more likely to become contaminated by unhygienic vessels than screw-cap closed containers which do not require the use of such vessels [26,28]. The inability of government at the different tiers to meet the increasing water demand in Nigeria leading to people resorting to the use of untreated or inadequately treated surface and ground water and the need for storing sourced water at the household level with the concomitant health risks necessitated this study. To ensure safe water at the household point of consumption, sources of contaminants have to be verified and prevented [30], hence, this study is aimed at comparing the physicochemical and microbiological qualities of source and stored water in some selected locations in western part of Nigeria.

2. Materials and Methods

2.1. Study Area and Sampling

Samples of source and stored water were collected from three different States (Osun, Oyo and Lagos, Southwest Nigeria). The study area is distributed within four selected local governments (LG) from each state. Lagos, Osun and Oyo states are located between longitudes 4°1′E and 5°31′30″E and latitudes 7°12′N and 8°32′30″N. (Figure 1).
One hundred and eighty water samples were collected from 120 houses in four LG each of three states (Lagos: Mushin, Odi-Olowo, Surulere, Yaba; Oyo: Akinyele, Ibadan North, Ibadan North-West, Ibadan South-East and Osun: Ife Central, Ife East, Ife North, Ife South). Water samples both source (well (60), spring (15), borehole (30) and municipal water (15)) and stored (60) were collected in duplicates. Sampling of well water, however, constitutes the major source of drinking water in these areas. Most of the wells were not less than 10 years old, privately owned and are usually open to general public. Half of the numbers of the studied wells were covered while the others were not. Drawing of water from these wells was done by the use of 5–7 L containers, which is tied directly to the well cover. In certain cases where this is not possible, individual fetcher usually comes with small bucket to draw water. Aside from well water, some of the surface waters used as source water are as shown in supplementary material (Figures S1 and S2). These are mostly untreated and exposed to debris and contamination through various anthropogenic activities.
Samples were collected aseptically in the morning using the sampling and storage procedures according to [31]. All samples were collected in 1000 mL sterile sample bottles and immediately transported in cooler boxes from sample sites to the laboratory for analysis within 24 h [32].

2.2. Physicochemical Analyses of Collected Samples

The samples were analysed for physical and chemical water quality parameters as described by FAO [33]. The sample temperature, pH and total dissolved solids (TDS) were determined at the point of sampling using portable hand pH meter (Hanna instruments, Beijing, China), mercury thermometer (model 275-k) and digital TDS-meter (Hanna instruments model TDS-02/TDS-03) respectively. Turbidity was also measured at point of collection by measuring the absorbance of the sample at 540 nm wavelength using colorimeter.
Off-sites parameters: biological oxygen demand BOD and dissolved oxygen (DO) were evaluated using standard titrimetric methods [32]. Calcium and magnesium ion contents were analysed using PerkinElmer 400 Atomic Absorption Spectrophotometer (Ohio, USA) at different wavelengths (422.67 nm, and 589.21 nm respectively) [34]. Total hardness (Y) of the water was determined by calculation method described by Ademoroti [35] using this formula: 2.5 × (Ca++ mg/L) + 4.1 × (Mg++ g/L) = Y (mg CaCO3).

2.3. Microbiological Analyses of Collected Samples

Total coliform (TC), faecal coliform (FC), faecal enterococci (EC), heterotrophic plate count (HPC) and fungi count of samples were determined using membrane filtration technique [32]. Aliquot of 100 mL from each sample was filtered through sterile Millipore filter papers (porosity of 0.45 μm) in a membrane filter apparatus. After filtration, the filter papers were transferred aseptically onto plates containing sterile absorbent pad soaked with different broths (m-Endo broth, m-FC broth with Rosolic acid, m-KF-Streptococcal broth, m-HPC broth and Y. M. green broth respectively.
Plates were incubated in an inverted position for the growth of thermo-tolerant faecal coliforms at 44.5 °C for 24 h ± 2 h, TC, EC and HPC at 35 °C for 48 h, and fungi at 20–28 °C for 5–7 days [32]. Pure cultures of isolates obtained were subjected to standard morphological and biochemical tests to identify bacterial and fungal isolates respectively [35,36].

2.4. Statistical Analysis

Data collected were subjected to One-way analysis of variance (ANOVA) using Statistical Package for Social Sciences, SPSS Version 20 software (IBM, New York, USA). Comparison were done to assess whether samples varied significantly between sampling points and point of use or storage, possibilities less than 0.05 (p < 0.05) were considered statistically.

3. Results

3.1. Physicochemical Analysis of Source and Stored Samples

The results of the physicochemical parameters of analyzed samples are shown in Table 1. In Osun state, the mean values of the stored water were higher than the source in all these parameters: pH, temperature, DO, BOD, total hardness except turbidity and TDS. In Oyo and Lagos state, only two parameters (DO and pH) had higher values for stored water while in other parameters the values for source water were greater than the stored water. The acidity and alkalinity (pH) level of samples ranged between 6.4 ± 0.2 to 6.9 ± 0.1 (slightly acidic) with the lowest in Osun source water and highest in Lagos stored water. There was significant difference between pH of source and stored water in all the three states Table 1. The mean temperature values obtained in this study ranged from 15.8 ± 0.2–25.0 ± 0.9°C. There was no significant difference in temperature of source compared with stored water only in Oyo state. The mean turbidity value ranged from 10.4 ± 1.0–24.3 ± 2.6 NTU, the lowest was mean stored water in Osun while the highest was mean source water in Oyo state. Turbidity was highly significant in all the states sampled and well above acceptable limits of <5 NTU. Total dissolved solids in all the areas were within acceptable limits although there was significant difference between the stored and source waters. There was no significant difference in mean values of DO between stored and source waters but all values were below acceptable limits. Total hardness was within limit in all the samples and there was no significant difference when stored water is compared with sources.

3.2. Microbial Analysis of Source and Stored Samples

The mean values of microbiological parameters (HPC, TC, EC, FC and fungi count) obtained for stored water were higher than the mean values of their sources, however, there was no significant difference in means of all microbiological parameters between stored and source waters except HPC and FC in Oyo state (Table 2). The breakdown of microbiological parameters in each state is shown as Table S1 in supplementary data. The isolated bacteria are as shown in Table 3. Enterococcus faecalis were isolated from samples in all selected locations. Klebsiella pneumoniae and Pseudomonas aeruginosa were highly prevalent in all the three states. Enterobacter aerogenes was also significantly prevalent in all the states however, the highest occurring bacteria in Lagos is Salmonella sp., P. aeruginosa in, Osun and Oyo respectively. Table 4 indicated the diversity of fungi isolated from analyzed samples in which Mucor janssenii was present in all samples collected from selected locations. Trichoderma harzianum is the most prevalent fungi in the samples from Osun state and Oyo while in Lagos state samples Mucor janssenii had the highest prevalence level.

4. Discussion

The survival of microorganisms in waters is highly influenced by many environmental factors such as temperature, salinity, pH, turbidity and supply of organic matter as nutrients [37]. The measure of concentration of hydrogen and hydroxyl ion is an important index of acidity or alkalinity [38]. The average pH value of samples (source and stored) from the study areas fell within standard (6.5–8.5) stipulated international limits. The increase in pH values of the stored water above sources could be as a result of the activities of the resident flora and or their death which results in the release of inorganic substances such as ammonia [39]. Changes in pH are known to be a resultant of processes such as photosynthesis, respiration, temperature exposure to air, disposal of industrial wastes, geology and mineral content of a catchment area, acid mine drainage, agricultural runoff, carbon dioxide concentration in the atmosphere, and accumulation and decomposition of organic detritus in the water producing weak carbonic acids that impact on pH [40]. Furthermore, the increase in the mean values of the DO (stored water) might be as a result of exposure of the containers to air during storage [41]. Dissolved oxygen (DO) serves as an indicator of the biological health of a water body.Dissolve oxygen levels can fluctuate throughout the day and are affected by changes in water temperature, the concentration of organic materials (i.e., industrial or municipal wastes can increase the concentration of organic matter) [42]. Water turbidity is very important because high turbidity is often associated with higher level of disease causing microorganism such as bacteria and other parasites [43]. Also, turbidity levels are dependent on the amount of suspended particles present in the water. Suspended particles act as a substrate for microorganisms in the water, thus promoting growth of the microorganism populations. The increase in mean values of the turbidity (source water) is an indication of pollution which enhances increase in number of disease causing microorganisms. Water with excess TDS can reduce water clarity, hereby harbouring microorganisms of health importance [44]. High mean values of TDS (source water) might be as a result of pollution which also enhances the growth of microorganism. Chemical contaminant can pose public health problem after prolonged exposure in particular those that can bio-accumulate.
High mean HPC, TC and FC values that was observed in stored water might be as a result of contamination from their untidy or unclean storage facilities, interaction of the little children with the water, insertion of dirty container to collect or remove water from the storage container, uncovered containers which are prone to environmental conditions such as dust and dirt [45]. Furthermore, in EC count, the high values observed in stored water indicated contamination which might be as a result of low level of sanitation facilitated by hands during defecation or other activities, using of unclean materials in getting water from the storage etc. Pickering et al. [46] reported a positive correlation between enterococci on hands and enterococci in stored drinking water in households in peri-urban Dar es Salaam. Pickering et al. [47] suggested that post-collection contamination of stored waters in areas with low levels of sanitation could be facilitated by hands contaminated during defecation or other activities. The isolation of these potential pathogenic organisms such as E. coli, K. pneumonia, P. aeruginosa, E. aerogenes, Salmonella sp., Aspergillus sp., Mucor sp., Rhizopus sp. and Candida sp. (Table 3 and Table 4) from analyzed samples in this study is an indication of poor hygiene and sanitation on the part of the users and pose health risks. The presence of E. coli and opportunistic pathogens in some of the samples indicated recent faecal contamination and is of major health importance.Similar studies were carried out by Schets et al. [48] who analyzed quality of drinking water from private water supplies in Netherland, the result showed that 10.9% samples were contaminated due to faecal organisms such as E. coli and Enterococcus species. Filamentous fungi (mainly of the genera Aspergillus, Mucor and Rhizopus (Table 4) are typically more prevalent than yeast and yeast-like fungi. This high prevalence level is in agreement with Göttlich et al. [49] and Patterson et al. [50]. Aspergillus sp. was observed dominating among the fungi isolated from the samples. Presence of some of the fungi may put consumers of such water at risk of infections such as aspergillosis, hypersensitivity pneumonitis, extrinsic allergic alveolitis, and opportunistic infections such as parlous disease caused by Rhizopus sp. [51,52,53].

5. Conclusions

Water of poor quality is a threat to the health and wellbeing of the populace. The study examined the physical, chemical and microbiological parameters of household stored domestic water and their corresponding sources. Improper handling of both source and stored water were observed and this is due to poor hygiene and sanitation level of the handlers.Therefore, it is recommended that both stored and source water should be from good quality sources such as adequately treated municipal water supply, deep boreholes andstorage should be in clean covered containers preferably with tap.Also, the periodic cleaning and disinfection of the storage facilities is highly desirable in order to prevent contamination.

Supplementary Materials

The following are available online at https://www.mdpi.com/2071-1050/9/3/454/s1, Table S1: Mean values of different microbiological parameters on samples by the selected LGA in Oyo, Osun and Lagos State; Figure S1: Gbaro spring water Olode (Ife South); Figure S2: Alum water Olode (Ife South).

Acknowledgments

We are grateful to the Heads of department of Microbiology, Obafemi Awolowo University, Ile-Ife and Biological Sciences, Yaba College of Technology (YCT), Lagos, Nigeria where laboratory analysis were conducted. We are also grateful to members of the local government communities who provided information in this study. The support received from Adetoro of Chemistry department YCT would not go unmentioned. The financial support of the South Africa Medical Research Council is appreciated.

Author Contributions

Mary Bisi-Johnson conceived of the study, participated in the design and coordination of the study, participated in field work and preparation of the manuscript. Kehinde Adediran participated in field and laboratory work and drafting of the manuscript. Adekunle Akinola performed the experiments, analyzed the data and also drafted the manuscript. Oluseun Popoola was involved in some aspects of sampling, laboratory coordination and physicochemical analysis. Anthony Okoh assisted with the concept and design of the study, provided technical advice and revised manuscript. All authors read and approved the final manuscript.

Conflicts of Interest

The authors declare that they have no conflicts of interests on this work.

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Figure 1. Demographic representation of sampled areas. Key: AKN = Akinyele; IBN = Ibadan North; IBSE = Ibadan South East; IBNW = Ibadan North West; IFC = Ife Central; IFN = Ife North; IFE = Ife East; IFS = Ife South; MUS = Mushin; SUR = Surulere; YAB = Yaba; ODI = Odi-Olowo.
Figure 1. Demographic representation of sampled areas. Key: AKN = Akinyele; IBN = Ibadan North; IBSE = Ibadan South East; IBNW = Ibadan North West; IFC = Ife Central; IFN = Ife North; IFE = Ife East; IFS = Ife South; MUS = Mushin; SUR = Surulere; YAB = Yaba; ODI = Odi-Olowo.
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Table 1. Mean values of the physicochemical parameter of water samples in Oyo, Osun and Lagos State.
Table 1. Mean values of the physicochemical parameter of water samples in Oyo, Osun and Lagos State.
ParameterOyo StateOsun StateLagos State WHO Limits
Stored (45)Source (15)FPStored (45)Source (15)FPStored (45)Source (15)FP
pH6.9 ± 0.16.8 ± 0.10.10.746.5 ± 0.26.4 ± 0.20.30.586.9 ± 0.16.7 ± 0.12.00.166.5–8.5
Temperature(°C)16.7 ± 0.415.8 ± 0.24.70.0324.5 ± 0.825.0 ± 0.90.20.6624.5 ± 0.324.5 ± 0.30.01.0025–30
TDS (mg/L)355.3 ± 22.4373.2 ± 23.20.30.58349.9 ± 29.2376.0 ± 26.00.50.51368.3 ± 20.9377.1 ± 21.20.10.77500
Turbidity(NTU)19.4 ± 2.224.3 ± 2.62.10.3010.4 ± 1.012.7 ± 1.22.60.1617.0 ± 2.120.8 ± 2.71.30.26<5
DO(mgO2/L)1.2 ± 0.11.1 ± 0.10.01.03.6 ± 0.32.9 ± 0.23.80.064.8 ± 0.24.3 ± 0.41.10.30≥5
BOD(mgO2/L)0.8 ± 0.10.8 ± 0.10.00.901.6 ± 0.11.2 ± 0.13.80.052.7 ± 0.22.7 ± 0.30.00.90-
Total hardness(mg/L)177.3 ± 15.7202.2 ± 18.11.10.30134.2 ± 12.1118.2 ± 12.00.90.3567.5 ± 12.251.8 ± 9.51.00.31500
Calcium ion (mg/L)34.2 ± 3.238.9 ± 3.80.90.3524.5 ± 2.921.9 ± 3.30.60.5613.1 ± 2.27.6 ± 1.44.50.03-
Magnesium ion(mg/L)23.0 ± 2.325.7 ± 2.50.60.4417.9 ± 1.715.3 ± 1.31.70.219.5 ± 1.36.4 ± 0.84.40.04-
Table 2. Mean values of different microbiological parameters on water samples from Oyo, Osun and Lagos States.
Table 2. Mean values of different microbiological parameters on water samples from Oyo, Osun and Lagos States.
States OsunLagosOyoWHO Limits
Points of Collection (No. of Samples) Stored (45)Source (15)FPStored (45)Source (15)FPStored (45)Source (15)FP
Microbial ParametersHeterotrophic Plate Count (cfu/100mL)140.9 ± 13.482.0 ± 10.711.80.00112.3 ± 13.377.4 ± 7.95.10.03152.6 ± 15.280.9 ± 7.617.80.75<10
Faecal Coliform Count (cfu/100mL))42.0 ± 7.09.6 ± 2.518.80.0012.8 ± 6.81.4 ± 0.52.80.1014.48 ± 6.0710.0 ± 4.20.40.550
Enterococci Count (cfu/100mL)59.1 ± 8.824.9 ± 4.711.80.0026.3 ± 7.916.7 ± 5.21.00.3249.28 ± 11.3412.0 ± 3.31.00.000
Total Coliform Count (cfu/100mL)46.2 ± 7.112.3 ± 3.318.90.0072.0 ± 15.549.9 ± 10.51.40.2428.40 ± 9.7011.3 ± 4.72.50.12no limit given
Fungal Count (cfu/100mL)109.6 ± 12.546.0 ± 6.021.00.0093.6 ± 8.4111.5 ± 7.12.70.1199.58 ± 9.2255.9 ± 3.419.80.00no limit given
Table 3. Bacteria Isolated from Sampled Areas.
Table 3. Bacteria Isolated from Sampled Areas.
Sampled AreaIsolated Bacteria
Citrobacter fruendiiSerratia marcescensProteus mirabilisSalmonella sp.Bacillus sp.Shigella sp.Escherichia coliVibrio choleraePseudomonas aeruginosaEnterococcus faecalisKlebsiella pneumoniaeAeromonas sp.Micrococcus sp.Enterobacter aerogenesStaphylococcus aureus
Osun State✓✓✓✓✓✓✓✓✓✓
Lagos State✓✓✓✓✓✓
Oyo State✓✓✓✓✓✓
KEYS: (★) No Isolate; (✓) Low prevalence; (✓✓) Moderate prevalence level; (✓✓✓) High prevalence level.
Table 4. Fungi Isolated from Sampled Areas.
Table 4. Fungi Isolated from Sampled Areas.
Sampled AreaIsolated Fungi
Candida kruseiCandida parapsilosisCandida albicansRhizopus stoloniferMucor jansseniiTrichoderma viridaeTrichoderma harzianumRhizoctonia solaniAspergillus nigerA. brevipesA. parasiticusA. wentiiA. fumigatusPenicillium roqueforti
Osun State✓✓✓✓✓✓✓✓
Lagos State✓✓✓✓✓
Oyo State✓✓✓✓✓✓✓
KEYS: (★) No Isolate; (✓) Low prevalence; (✓✓) Moderate prevalence level; (✓✓✓) High prevalence level; (✓✓✓✓) Very high prevalence level.
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