Phyllosphere and Carposphere Bacterial Communities in Olive Plants Subjected to Different Cultural Practices

Pascazio, Silvia; Crecchio, Carmine; Ricciuti, Patrizia; Palese, Assunta Maria; Xiloyannis, Cristos; Sofo, Adriano

doi:10.4081/pb.2015.6011

Open AccessArticle

Phyllosphere and Carposphere Bacterial Communities in Olive Plants Subjected to Different Cultural Practices

by

Silvia Pascazio

¹,

Carmine Crecchio

¹,

Patrizia Ricciuti

¹,

Assunta Maria Palese

²,

Cristos Xiloyannis

² and

Adriano Sofo

^3,*

¹

Department of Soil, Plant and Food Sciences, University of Bari, Aldo Moro, Bari 70121, Italy

²

Department of European and Mediterranean Cultures: Architecture, Environment and Cultural Heritage, Basilicata University, 75100 Matera, Italy

³

School of Agricultural, Forestry, Food and Environmental Sciences, Basilicata University, Viale dell'Ateneo Lucano 10, 85100 Potenza, Italy

^*

Author to whom correspondence should be addressed.

Int. J. Plant Biol. 2015, 6(1), 6011; https://doi.org/10.4081/pb.2015.6011

Submission received: 13 May 2015 / Revised: 28 May 2015 / Accepted: 28 May 2015 / Published: 7 September 2015

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Abstract

The aim of this study was to characterize phyllosphere and carposphere bacterial communities of olive trees subjected for 13 years to two different soil management systems (sustainable and conventional) in a mature olive grove located in Southern Italy. Amplified DNA fragments of the 16S ribosomal RNA eubacterial gene (16S rRNA) of bacteria living on leaf and fruit surface, and in fruit pulp were analyzed by denaturing gradient gel electrophoresis (DGGE). A clone library of 16S rRNA amplicons extracted from the bacteria living in pulp homogenates and a phylogenetic analysis were performed. Generally, the DGGE patterns of the bacteria from both the treatments clustered separately. The medium-term sustainable orchard management resulted in a higher number of bacterial species from olive fruit pulp. Phyllosphere and carposphere communities evaluated by DGGE were affected by the type of the agricultural practices adopted. A better understanding of phyllosphere and carposphere microbiota of cultivated olive plants could be useful for the promotion of plant growth, a better plant protection and a higher crop quality.

Keywords: bacterial diversity; carposphere; endophytic bacteria; Olea europaea L.; phyllosphere; sustainable soil management

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MDPI and ACS Style

Pascazio, S.; Crecchio, C.; Ricciuti, P.; Palese, A.M.; Xiloyannis, C.; Sofo, A. Phyllosphere and Carposphere Bacterial Communities in Olive Plants Subjected to Different Cultural Practices. Int. J. Plant Biol. 2015, 6, 6011. https://doi.org/10.4081/pb.2015.6011

AMA Style

Pascazio S, Crecchio C, Ricciuti P, Palese AM, Xiloyannis C, Sofo A. Phyllosphere and Carposphere Bacterial Communities in Olive Plants Subjected to Different Cultural Practices. International Journal of Plant Biology. 2015; 6(1):6011. https://doi.org/10.4081/pb.2015.6011

Chicago/Turabian Style

Pascazio, Silvia, Carmine Crecchio, Patrizia Ricciuti, Assunta Maria Palese, Cristos Xiloyannis, and Adriano Sofo. 2015. "Phyllosphere and Carposphere Bacterial Communities in Olive Plants Subjected to Different Cultural Practices" International Journal of Plant Biology 6, no. 1: 6011. https://doi.org/10.4081/pb.2015.6011

APA Style

Pascazio, S., Crecchio, C., Ricciuti, P., Palese, A. M., Xiloyannis, C., & Sofo, A. (2015). Phyllosphere and Carposphere Bacterial Communities in Olive Plants Subjected to Different Cultural Practices. International Journal of Plant Biology, 6(1), 6011. https://doi.org/10.4081/pb.2015.6011

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Phyllosphere and Carposphere Bacterial Communities in Olive Plants Subjected to Different Cultural Practices

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