Sterol Profile in Leaves of Spring Oats (Avena sativa L.) Under Conditions of the Cryolithozone

Round 1

Reviewer 1 Report

Comments for Authors

The current research paper entitled “Sterol Profile of Spring Oats (Avena sativa L.) under Autumn Cold in Cryolithozone Conditions” has been reviewed. The results of this research provide valuable insight into the molecular dynamics of sterols and triterpenoids in Avena sativa (spring oat) leaves under varying sowing conditions, contributing to the understanding of plant adaptations to environmental stress.

This article is commended for its innovative approach to identifying novel sterols and triterpenoids in the leaves of Avena sativa and for providing important insights into the plant's metabolic adaptations to extreme climatic conditions, thereby advancing our understanding of plant survival. There is a good deal of work in the manuscript, but minor changes need to be made. Here are some;

lines 76-81 – the introduction should end with a clearly formulated research objective resulting from the literature review, the authors should also indicate what new information their research results bring to the knowledge they already have.

Table 1, the value of the linear correlation coefficient could be given,

 

Ideally, conclusions should focus on the overall outcome of the study and be more targeted. In this section, authors summarize the results. It is important to include numerical values along with the mean findings in the conclusion.

 

Conclusion line 467-480, It is important that conclusions focus on the overall outcome of the study and are more targeted. The present section is a summary of the results. The conclusion should include numerical values along with mean findings.

It is generally considered that written English is of good quality. The current research paper, however, could be proofread to correct grammatical, sentence structure, and conceptual errors.

Comments for Authors

The current research paper entitled “Sterol Profile of Spring Oats (Avena sativa L.) under Autumn Cold in Cryolithozone Conditions” has been reviewed. The results of this research provide valuable insight into the molecular dynamics of sterols and triterpenoids in Avena sativa (spring oat) leaves under varying sowing conditions, contributing to the understanding of plant adaptations to environmental stress.

This article is commended for its innovative approach to identifying novel sterols and triterpenoids in the leaves of Avena sativa and for providing important insights into the plant's metabolic adaptations to extreme climatic conditions, thereby advancing our understanding of plant survival. There is a good deal of work in the manuscript, but minor changes need to be made. Here are some;

lines 76-81 – the introduction should end with a clearly formulated research objective resulting from the literature review, the authors should also indicate what new information their research results bring to the knowledge they already have.

Table 1, the value of the linear correlation coefficient could be given,

 

Ideally, conclusions should focus on the overall outcome of the study and be more targeted. In this section, authors summarize the results. It is important to include numerical values along with the mean findings in the conclusion.

 

Conclusion line 467-480, It is important that conclusions focus on the overall outcome of the study and are more targeted. The present section is a summary of the results. The conclusion should include numerical values along with mean findings.

It is generally considered that written English is of good quality. The current research paper, however, could be proofread to correct grammatical, sentence structure, and conceptual errors.

 

Comments for author File: Comments.pdf

Author Response

Response to Reviewer

Authors: We would like to thank the reviewer for their valuable comments that we have used to improve the quality of our manuscript. We appreciate your input and agree with all your comments. We have tried to make the necessary corrections to address the shortcomings and improve the overall quality of the paper. Corrections made in the text are highlighted in green. Below are our detailed responses to each comment.

 

Reviewer

The current research paper entitled “Sterol Profile of Spring Oats (Avena sativa L.) under Autumn Cold in Cryolithozone Conditions” has been reviewed. The results of this research provide valuable insight into the molecular dynamics of sterols and triterpenoids in Avena sativa (spring oat) leaves under varying sowing conditions, contributing to the understanding of plant adaptations to environmental stress.

 

This article is commended for its innovative approach to identifying novel sterols and triterpenoids in the leaves of Avena sativa and for providing important insights into the plant's metabolic adaptations to extreme climatic conditions, thereby advancing our understanding of plant survival. There is a good deal of work in the manuscript, but minor changes need to be made. Here are some;

 

Reviewer: lines 76-81 – the introduction should end with a clearly formulated research objective resulting from the literature review, the authors should also indicate what new information their research results bring to the knowledge they already have.

Authors: Thank you for your advice. We have added the purpose of the work to the manuscript.

 

Reviewer: Table 1, the value of the linear correlation coefficient could be given,

Authors: Table 1 shows the phenological phases of oat development and weather conditions (temperature, precipitation). The time frame was chosen depending on the phase of plant development and the air temperature in September and October.

 

 

Reviewer: Ideally, conclusions should focus on the overall outcome of the study and be more targeted. In this section, authors summarize the results. It is important to include numerical values along with the mean findings in the conclusion.

Authors: Done. Thank you for your advice. We have completely changed the conclusion according to your comments.

 

Reviewer: Conclusion line 467-480, It is important that conclusions focus on the overall outcome of the study and are more targeted. The present section is a summary of the results. The conclusion should include numerical values along with mean findings.

Authors: Done. Thank you for your advice. We have completely changed the conclusion according to your comments.

 

Reviewer: It is generally considered that written English is of good quality. The current research paper, however, could be proofread to correct grammatical, sentence structure, and conceptual errors.

Authors: Done. All corrections are highlighted in color.

Author Response File: Author Response.docx

Reviewer 2 Report

The authors have investigated the sterol and triterpene profile of  Avena sativa grown under  Cold weather  in Cryolithozone Conditions. The manuscript is interesting However, its novelty is relatively low since the authors have published and discussed a similar idea in their paper :  https://doi.org/10.3390/plants13081076 , the only difference is the type of compounds analyzed. 

*The language needs improvement , some grammatical mistakes and typos were detected for example:  under Autumn  Cold is the title should be cold Autumn,   line 21 the verb was should be were, line 54 please separate the two words campsterolreduce ,line 130 the expression should be samples of A. sativa leaves, line 138 it should be the resulted extracts not resulting, in line 180 use either of the verbs carried out or conducted , in line 183 correct the word day.

* in section 2.2 please revise the heading 

* please rephrase line 91-98 as it is not clear

* In table 3 please add the RI for each compound

 

Author Response

Response to Reviewer

Authors: We would like to thank the reviewer for their valuable comments that we have used to improve the quality of our manuscript. We appreciate your input and agree with all your comments. We have tried to make the necessary corrections to address the shortcomings and improve the overall quality of the paper. Corrections made in the text are highlighted in green. Below are our detailed responses to each comment.

 

Reviewer:

Major comments

The authors have investigated the sterol and triterpene profile of Avena sativa grown under  Cold weather  in Cryolithozone Conditions. The manuscript is interesting However, its novelty is relatively low since the authors have published and discussed a similar idea in their paper :  https://doi.org/10.3390/plants13081076 , the only difference is the type of compounds analyzed.

 

Authors:

Thank you for your comments and constructive criticism.

Under natural conditions of Central and Northeastern Yakutia, a significant part of wild fodder plants in cereal–sedge phytocenoses of shallow valley lands do not have sufficient time to complete the growth–development cycle; they are covered by snow while remaining green and form a valuable cryo-feed for wild animal and livestock (the Yakut horse, reindeer, etc.) under conditions of prolonged and extremely cold winter (Petrov et al., 2017, doi: 10.15389/agrobiology.2017.6.1129rus; Safronov et al., 2024 doi :10.31242/2618-9712-2024-29-3-470-478). Taking into account the above, the spring oat plants of late sowing date are considered as the main crop for the production of green matter for silage or cryo-feed for livestock in the permafrost zone (Petrov et al., 2017, doi: 10.15389/agrobiology)/2017.6.1129rus). 

On the other hand, phytosterols are known to be environmentally friendly feed additives that stimulate animal growth and positively affect the immune status of their organism (Luo et al., 2015, https://doi.org/10.3390/md13074231Feng et al., 2018, https://doi.org/10.1016/j.bbalip.2018.08.004). Therefore, information on the dynamics of changes in STs in leaves at different sowing dates of spring oats have of practical interest in order to assess the beneficial properties of this species for humans and animals. Vilensky oat variety was selected for our experiments. Among 3 varieties cultivating in Yakutia (Pokrovsky, Pokrovsky 9 and Vilensky) from the State Register of Breeding Achievements in the East Siberian region, Vilensky oat variety showed the higher average yield, moderately susceptibility to dusty and durum bunt. Beside it was poorly affected by bacterial blight, red-brown spot and stem rust than Pokrovsky, Pokrovsky 9 varieties (Petrova, and Osipova, 2019, doi:10.24411/2587-6740-2019-11011).

The purpose of this work is to study the structural diversity of STs in spring oat (A. sativa) leaves as a function of different sowing dates and identify the factors that determine the composition of these membrane components and their possible role in the process of cold adaptation in the cryolithozone.

The main difference of this manuscript is that we compared two different sowing dates oat with each other for composition and sterol content.

 

Detail comments

Reviewer: *The language needs improvement , some grammatical mistakes and typos were detected for example:  under Autumn  Cold is the title should be cold Autumn,  

 

Authors: Thank you, we have changed the title of the manuscript.

Sterol Profile in Leaves of Spring Oats (Avena sativa L.) under Conditions of the Cryolithozone

 

Reviewer: line 21 the verb was should be were,

Authors: Done. Thank you for pointing out our oversight

 

Reviewer: line 54 please separate the two words campsterolreduce ,

Authors: Done.

 

Reviewer: line 130 the expression should be samples of A. sativa leaves,

Authors: Done.

 

Reviewer: line 138 it should be the resulted extracts not resulting,

Authors: Done.

 

Reviewer: in line 180 use either of the verbs carried out or conducted ,

Authors: Done.

 

Reviewer: in line 183 correct the word day.

Authors: Done.

 

Reviewer: * in section 2.2 please revise the heading 

Authors: Done.

 

Reviewer: * please rephrase line 91-98 as it is not clear

Authors: Done.

 

Reviewer: * In table 3 please add the RI for each compound

Authors: Done. Thank you for the advice. The table contains the RRT and RI  data for each component.

 

 

Author Response File: Author Response.docx