Magnesium Hydroxide Microparticle Treatment Potently Inhibits Venturia oleaginea Pathogenesis on Olives

Round 1

Reviewer 1 Report

Magnesium Hydroxide Microparticle Treatment Potently Inhibits Venturia oleaginea Pathogenesis on Olives

In my observation, I believe the present work cab be published in the international Journal of plant biology after major revision

 

 

The novelty of the work is in acceptable range. Although Mg(OH)2 microparticles are already know for plant treatment such as Tomatoes are well studies., Authors did very intensive studies on Olive tress leaf. However, before considering for publication, it needs major revision because

 

1)      Need to study the characterization of micro Mg(OH)2 using the following state-of-art technics

a)       TEM

b)      XPS

c)       TGA

2)      English Language and grammar

Magnesium Hydroxide Microparticle Treatment Potently Inhibits Venturia oleaginea Pathogenesis on Olives

In my observation, I believe the present work cab be published in the international Journal of plant biology after major revision

 

 

The novelty of the work is in acceptable range. Although Mg(OH)2 microparticles are already know for plant treatment such as Tomatoes are well studies., Authors did very intensive studies on Olive tress leaf. However, before considering for publication, it needs major revision because

 

1)      Need to study the characterization of micro Mg(OH)2 using the following state-of-art technics

a)       TEM

b)      XPS

c)       TGA

2)      English Language and grammar

Author Response

Dear Reviewer 1,

Thank you very much for your contibution.

Please see the attachment for our responce.

Author Response File: Author Response.docx

Reviewer 2 Report

This article investigates the use of magnesium hydroxide porous micron-scale particles (Mg(OH)2 PMP) as a potent treatment for controlling Olive Leaf Spot (OLS) disease, which is caused by the fungus Venturia oleaginous in olive trees. A comprehensive analysis was performed, including assessment of disease symptoms, fungal load quantification, fungal community profiling, and plant gene expression, providing a multi-faceted evaluation of the treatment. The article is well-written and investigates a new study. However, I have some concerns.

This article investigates the use of magnesium hydroxide porous micron-scale particles (Mg(OH)2 PMP) as a potent treatment for controlling Olive Leaf Spot (OLS) disease, which is caused by the fungus Venturia oleaginous in olive trees. A comprehensive analysis was performed, including assessment of disease symptoms, fungal load quantification, fungal community profiling, and plant gene expression, providing a multi-faceted evaluation of the treatment. The article is well-written and investigates a new study. However, I have some concerns. 1. Clearly discuss or write about the aim, novelty, and significance of your study at the end of the introduction section in the last paragraph. 2. Provide more information on the copper fungicide treatment used for comparison, such as the active ingredient, concentration, and application method. 3. Describe the disease severity scoring system used to assess symptoms on the trees. 4. Add data about DSI and control effect. 5. Explain how the fungal load quantification was performed (e.g. qPCR, CFU counts). 6. Clearly summarize the key findings and their implications in the conclusions section and write about future research work of study. 7. Add a figure showing the dose-dependent effects of Mg(OH)2 PMP on Venturia growth in vitro 8. Explain how the Mg(OH)2 PMP treatment could be integrated into existing olive disease management programs.

Author Response

Dear Reviewer 2,

Thank you very much for your contibution.

Please see the attachment for our responce.

Author Response File: Author Response.docx

Reviewer 3 Report

This article systematically explains one problem clearly, and the work is done very well, but there are still several issues that need to be noted：

Abstract: The abstract section needs to be condensed. The paper provides a very systematic and in-depth evaluation of the application of magnesium hydroxide microparticle treatment in inhibiting Venturia oleaginea pathogenesis of olives, but it didn't really reflect it,. There is a lot of data in the text, which should be presented in the abstract.

Keywords: There is duplicate information between the six keywords, Olive tree and Olive leaf spot disease can be merged.

Materials and Methods 2.2. Plant spraying and sample collection: Is 2.9wt% the final spraying concentration? If so, as a drug that does not require dilution, the transportation cost will be very high; If not, how many times has it been diluted? The final spraying concentration should be clearly described; In addition, it is necessary to clarify whether the concentration of the copper spray is consistent or whether the spraying amount is consistent, otherwise it is impossible to compare their respective effects.

2.6. Quantification of fungal load: L241-242, Serial dilutions of the plasmid DNA, ranging from 241 100 pg to 0.01 pg, were used for the construction of the calibration curve. how to ensure the accuracy of the test results when the linear range of the standard curve reaches 4 orders of magnitude?

Conclusions  L671-672 “Previous investigation of Mg(OH) PMP had shown them to possess antimicrobial properties on the phyllosphere of tomato plants”, this sentence can be placed in the discussion section, and the conclusion is a summary of the research content of this article.

Author Response

Dear Reviewer 3,

Thank you very much for your contibution.

Please see the attachment for our responce.

Author Response File: Author Response.docx

Round 2

Reviewer 1 Report

As authors response the questions, I recommend publishing the work 

As authors response the questions, I recommend publishing the work 

Author Response

Dear Reviewer,

Thank you very much for taking the time to review this manuscript.

Kind Regards

Aggeliki Andreadelli

Reviewer 2 Report

The similarity rate is over 34%. Please reduce the manuscript similarity rate to <20%.

The similarity rate is over 34%. Please reduce the manuscript similarity rate to <20%.

Author Response

Dear Reviewer,

Thank you very much for taking the time to review this manuscript. 

I am attaching the similarity report for the revised manuscript generated by the DupliChecker tool.
Based on DupliChecker tool, the plagiarism is between 0-11%.   Kind Regards Aggeliki Andreadelli  

 

Author Response File: Author Response.pdf