Next Article in Journal
Effect of Low Power Microwave Radiation on Pigment Production in Bacteria
Previous Article in Journal
Molecular Investigation of Zoonotic Genotypes of Giardia intestinalis Isolates in Humans, Dogs and Cats, Sheep, Goats and Cattle in Araçatuba (São Paulo State, Brazil) by the Analysis of β-Giardin Gene Fragments
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Microbiology Research
Volume 5
Issue 1
10.4081/mr.2014.5402
microbiolres-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles thumb_up
...
Endorse textsms
...
Comment
Microbiology Research is published by MDPI from Volume 11 Issue 2 (2020). Previous articles were published by another publisher in Open Access under a CC-BY (or CC-BY-NC-ND) licence, and they are hosted by MDPI on mdpi.com as a courtesy and upon agreement with PAGEPress.
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Standardization of Lyophilization Medium for Streptococcus thermophilus Subjected to Viability Escalation on Freeze Drying

by
Rohit Sharma
1,2,*,
Bhagwan S. Sanodiya
1,
Gulab S. Thakur
1,
Pallavi Jaiswal
1,
Anjana Sharma
2 and
Prakash S. Bisen
1,2
1
Microbial Biotechnology Laboratory, Tropilite Foods Pvt. Ltd., Davars Campus, Gwalior, India
2
Department of Post Graduate Studies and Research in Biological Sciences, Rani Durgavati Vishwavidyalaya, Jabalpur, India
*
Author to whom correspondence should be addressed.
Microbiol. Res. 2014, 5(1), 5402; https://doi.org/10.4081/mr.2014.5402
Submission received: 25 March 2014 / Revised: 4 August 2014 / Accepted: 4 August 2014 / Published: 2 September 2014

Abstract

The objective of the present study is to develop a lyophilization medium for Streptococcus thermophilus (NCIM 2904) as the industrial exploitation of this bacterium totally depends upon preservation and lyophilization protocols. Protective effect of 18 compounds were observed individually and in combinations with different sugars, sugar alcohols, polymers, protein concentrates and buffers. Among all the protectants tested, ammonium citrate (1% w/w), K2HPO4 (1% w/w) and KH2PO4 (1% w/w) provided lowest protection to these bacterial cells while 10% (w/w) sodium caseinate, whey protein concentrate, sweet whey powder, and skim milk showed significant results in viability escalation. Survival in carbon sources like lactose, sucrose and maltodextrine was also favored maximally. Combination of sodium caseinate 10%, skim milk 5%, sucrose 5%, lactose 5% and mono sodium glutamate 1% in distilled water in ratio of 1:5 with S. thermophilus showed survival percentage of 96%.
Keywords: lyophilization; freeze drying; S. thermophilus; lyoprotectant; viability lyophilization; freeze drying; S. thermophilus; lyoprotectant; viability

Share and Cite

MDPI and ACS Style

Sharma, R.; Sanodiya, B.S.; Thakur, G.S.; Jaiswal, P.; Sharma, A.; Bisen, P.S. Standardization of Lyophilization Medium for Streptococcus thermophilus Subjected to Viability Escalation on Freeze Drying. Microbiol. Res. 2014, 5, 5402. https://doi.org/10.4081/mr.2014.5402

AMA Style

Sharma R, Sanodiya BS, Thakur GS, Jaiswal P, Sharma A, Bisen PS. Standardization of Lyophilization Medium for Streptococcus thermophilus Subjected to Viability Escalation on Freeze Drying. Microbiology Research. 2014; 5(1):5402. https://doi.org/10.4081/mr.2014.5402

Chicago/Turabian Style

Sharma, Rohit, Bhagwan S. Sanodiya, Gulab S. Thakur, Pallavi Jaiswal, Anjana Sharma, and Prakash S. Bisen. 2014. "Standardization of Lyophilization Medium for Streptococcus thermophilus Subjected to Viability Escalation on Freeze Drying" Microbiology Research 5, no. 1: 5402. https://doi.org/10.4081/mr.2014.5402

APA Style

Sharma, R., Sanodiya, B. S., Thakur, G. S., Jaiswal, P., Sharma, A., & Bisen, P. S. (2014). Standardization of Lyophilization Medium for Streptococcus thermophilus Subjected to Viability Escalation on Freeze Drying. Microbiology Research, 5(1), 5402. https://doi.org/10.4081/mr.2014.5402

Article Metrics

Back to TopTop