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Microbiology Research
Volume 3
Issue 1
10.4081/mr.2012.e11
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Article

Microbiologic Quality of an Untreated Water Sample Analyzed by a Novel DNA Chip for Simultaneous Detection of Microorganisms

by
Filipa F. Vale
1,*,
Kathy D. Saraiva-Pava
1,
Magda S.C. Fontes
1 and
Helena Vieira
2,3
1
Faculty of Engineering, Catholic University of Portugal, Rio de Mouro, Portugal
2
Bioalvo S.A., Edificio ICAT, Campus da FCUL, Lisbon, Portugal
3
Universidade de Lisboa, Faculdade de Ciências, Centre for Biodiversity, Functional and Integrative Genomics (BioFIG), Campus da FCUL, Campo Grande, Lisboa, Portugal
*
Author to whom correspondence should be addressed.
Microbiol. Res. 2012, 3(1), e11; https://doi.org/10.4081/mr.2012.e11
Submission received: 26 October 2011 / Revised: 9 January 2012 / Accepted: 8 February 2012 / Published: 30 March 2012

Abstract

Consumption of contaminated drinking water heavily contributes to the burden of gastrointestinal waterborne diseases. Conventional detection methodologies present several shortcomings, such as indirect measure of indicator species, low throughput and time consume. DNA chips have the potential to serve as surveillance systems for the simultaneous detection of pathogens overcoming these limitations. We have developed a DNA chip for simultaneous detection of multiple waterborne pathogens. Species specific DNA probes were implemented on a microarray, for microorganism detection. Present study reports the results of one untreated water sample analyzed by conventional methods and by the DNA chip. The results were concordant for the mandatory organisms (total coliforms, Escherichia coli, fecal enterococci) using both methods, reinforcing the utility and proof-ofconcept of the DNA chip. However, it is necessary a prior enrichment in a culture medium in order to obtain a positive signal using the DNA chip. The DNA chip may be a valuable distinctive tool for waterborne pathogens detection
Keywords: DNA chip; simultaneous microorganism’s detection; water quality; indicator bacteria; waterborne pathogen DNA chip; simultaneous microorganism’s detection; water quality; indicator bacteria; waterborne pathogen

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MDPI and ACS Style

Vale, F.F.; Saraiva-Pava, K.D.; Fontes, M.S.C.; Vieira, H. Microbiologic Quality of an Untreated Water Sample Analyzed by a Novel DNA Chip for Simultaneous Detection of Microorganisms. Microbiol. Res. 2012, 3, e11. https://doi.org/10.4081/mr.2012.e11

AMA Style

Vale FF, Saraiva-Pava KD, Fontes MSC, Vieira H. Microbiologic Quality of an Untreated Water Sample Analyzed by a Novel DNA Chip for Simultaneous Detection of Microorganisms. Microbiology Research. 2012; 3(1):e11. https://doi.org/10.4081/mr.2012.e11

Chicago/Turabian Style

Vale, Filipa F., Kathy D. Saraiva-Pava, Magda S.C. Fontes, and Helena Vieira. 2012. "Microbiologic Quality of an Untreated Water Sample Analyzed by a Novel DNA Chip for Simultaneous Detection of Microorganisms" Microbiology Research 3, no. 1: e11. https://doi.org/10.4081/mr.2012.e11

APA Style

Vale, F. F., Saraiva-Pava, K. D., Fontes, M. S. C., & Vieira, H. (2012). Microbiologic Quality of an Untreated Water Sample Analyzed by a Novel DNA Chip for Simultaneous Detection of Microorganisms. Microbiology Research, 3(1), e11. https://doi.org/10.4081/mr.2012.e11

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