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Microbiology Research
Volume 3
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10.4081/mr.2012.e8
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Article

Non-Detection of Chlamydia trachomatis Infection by Polymerase Chain Reaction in Pregnant Iranian Women

by
Parvin Hassanzadeh
1,
Hosein Sharifi
2,
Abdollah Bazargani
2,
Reza Khashei
2,
Amir Emami
2 and
Mohammad Motamedifar
2,3,*
1
Department of Biology, School of Science, Shiraz University, Shiraz, Iran
2
Department of Bacteriology and Virology, Medical School, Shiraz University of Medical Sciences, Shiraz, Iran
3
Shiraz HIV/Aids Research Center (SHARC), Shiraz University of Medical Sciences, Shiraz, Iran
*
Author to whom correspondence should be addressed.
Microbiol. Res. 2012, 3(1), e8; https://doi.org/10.4081/mr.2012.e8
Submission received: 31 October 2011 / Revised: 28 January 2012 / Accepted: 8 February 2012 / Published: 29 March 2012

Abstract

Chlamydia trachomatis is the most common cause of sexually transmitted infection. In 75% of women and 50% of men infection is asymptomatic. According to World Health Organization reports, the number of new genital infections with Chlamydia trachomatis reaches 100 million annually. The sensitivity and specificity of nacid amplification tests are 95% and 99%, respectively. Urine samples can provide a non-invasive method of testing for the detection of Chlamydia trachomatis by polymerase chain reaction (PCR). The aim of this study was to evaluate the prevalence of Chlamydia trachomatis infection in pregnant women referred to a teaching hospital affiliated to Shiraz University of Medical Sciences. Urine samples were obtained from 210 pregnant women and investigated microscopically and macroscopically by urinalysis. Precipitants were also used for DNA extraction and PCR test for detecting Chlamydia trachomatis. Among 210 urine specimens from women aged 15–39 years, none were positive for Chlamydia trachomatis by PCR. In spite of the high sensitivity and specificity of PCR, and the elimination of inhibitory effects on PCR test, no pregnant woman was positive for Chlamydia trachomatis. Here, we suggest that a larger sample should be studied and other sensitive methods could also be used in the future.
Keywords: polymerase chain reaction; Chlamydia trachomatis; Iran; pregnancy polymerase chain reaction; Chlamydia trachomatis; Iran; pregnancy

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MDPI and ACS Style

Hassanzadeh, P.; Sharifi, H.; Bazargani, A.; Khashei, R.; Emami, A.; Motamedifar, M. Non-Detection of Chlamydia trachomatis Infection by Polymerase Chain Reaction in Pregnant Iranian Women. Microbiol. Res. 2012, 3, e8. https://doi.org/10.4081/mr.2012.e8

AMA Style

Hassanzadeh P, Sharifi H, Bazargani A, Khashei R, Emami A, Motamedifar M. Non-Detection of Chlamydia trachomatis Infection by Polymerase Chain Reaction in Pregnant Iranian Women. Microbiology Research. 2012; 3(1):e8. https://doi.org/10.4081/mr.2012.e8

Chicago/Turabian Style

Hassanzadeh, Parvin, Hosein Sharifi, Abdollah Bazargani, Reza Khashei, Amir Emami, and Mohammad Motamedifar. 2012. "Non-Detection of Chlamydia trachomatis Infection by Polymerase Chain Reaction in Pregnant Iranian Women" Microbiology Research 3, no. 1: e8. https://doi.org/10.4081/mr.2012.e8

APA Style

Hassanzadeh, P., Sharifi, H., Bazargani, A., Khashei, R., Emami, A., & Motamedifar, M. (2012). Non-Detection of Chlamydia trachomatis Infection by Polymerase Chain Reaction in Pregnant Iranian Women. Microbiology Research, 3(1), e8. https://doi.org/10.4081/mr.2012.e8

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