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Development of a Novel Lipophilic, Magnetic Nanoparticle for in Vivo Drug Delivery

Section of Neurobiology, Biomedicine, Department of Health Science and Technology, Aalborg University, Fr. Bajers Vej 3B, 1.216, DK-9220 Aalborg East, Denmark
Author to whom correspondence should be addressed.
Pharmaceutics 2013, 5(2), 246-260;
Received: 25 February 2013 / Revised: 2 April 2013 / Accepted: 12 April 2013 / Published: 23 April 2013
(This article belongs to the Special Issue Drug Delivery Using Nanotechnology)
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The aim of the present study was to evaluate the transfection potential of chitosan-coated, green-fluorescent magnetic nanoparticles (MNPs) (chi-MNPs) after encapsulation inside polyethylglycol (PEG)ylated liposomes that produced lipid-encapsulated chitosan-coated MNPs (lip-MNPs), and also to evaluate how these particles would distribute in vivo after systemic injection. The transfection potential of both chi-MNPs and lip-MNPs was evaluated in vitro in rat brain endothelial 4 (RBE4) cells with and without applying a magnetic field. Subsequently, the MNPs were evaluated in vivo in young rats. The in vitro investigations revealed that the application of a magnetic field resulted in an increased cellular uptake of the particles. The lip-MNPs were able to transfect the RBE4 cells with an incidence of approximately 20% of a commercial transfection agent. The in vivo distribution studies revealed that lip-MNPs had superior pharmacokinetic properties due to evasion of the RES, including hepatic Kuppfer cells and macrophages in the spleen. In conclusion, we were able to design a novel lipid-encapsulated MNP with the ability to carry genetic material, with favorable pharmacokinetic properties, and under the influence of a magnetic field with the capability to mediate transfection in vitro. View Full-Text
Keywords: blood-brain barrier; endothelium; magnetofection; magnetic field; nanoparticle; transfection blood-brain barrier; endothelium; magnetofection; magnetic field; nanoparticle; transfection

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Linemann, T.; Thomsen, L.B.; Jardin, K.G.; Laursen, J.C.; Jensen, J.B.; Lichota, J.; Moos, T. Development of a Novel Lipophilic, Magnetic Nanoparticle for in Vivo Drug Delivery. Pharmaceutics 2013, 5, 246-260.

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