&alpha;/&beta;-Peptides as Nanomolar Triggers of Lipid Raft-Mediated Endocytosis through GM1 Ganglioside Recognition

Hetényi, Anasztázia; Szabó, Enikő; Imre, Norbert; Bhaumik, Kaushik Nath; Tököli, Attila; Füzesi, Tamás; Hollandi, Réka; Horvath, Peter; Czibula, Ágnes; Monostori, Éva; Deli, Mária A.; Martinek, Tamás A.

doi:10.3390/pharmaceutics14030580

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α/β-Peptides as Nanomolar Triggers of Lipid Raft-Mediated Endocytosis through GM1 Ganglioside Recognition

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Department of Medical Chemistry, University of Szeged, Dóm Tér 8, 6720 Szeged, Hungary

Institute of Genetics, Biological Research Centre, Temesvári krt. 62, 6726 Szeged, Hungary

Institute of Biophysics, Biological Research Centre, Temesvári krt. 62, 6726 Szeged, Hungary

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Synthetic and Systems Biology Unit, Biological Research Centre, Temesvári krt. 62, 6726 Szeged, Hungary

Authors to whom correspondence should be addressed.

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These authors contributed equally to this work.

Academic Editors: Prisca Boisguérin and Sébastien Deshayes

Pharmaceutics 2022, 14(3), 580; https://doi.org/10.3390/pharmaceutics14030580

Received: 27 January 2022 / Revised: 2 March 2022 / Accepted: 4 March 2022 / Published: 7 March 2022

(This article belongs to the Special Issue Current State of the Field of Cell-Penetrating Peptides as an Honorific Issue for Professor Ülo Langel)

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Abstract

Cell delivery of therapeutic macromolecules and nanoparticles is a critical drug development challenge. Translocation through lipid raft-mediated endocytic mechanisms is being sought, as it can avoid rapid lysosomal degradation. Here, we present a set of short α/β-peptide tags with high affinity to the lipid raft-associated ganglioside GM1. These sequences induce effective internalization of the attached immunoglobulin cargo. The structural requirements of the GM1-peptide interaction are presented, and the importance of the membrane components are shown. The results contribute to the development of a receptor-based cell delivery platform. View Full-Text

Keywords: cell delivery; glycan recognition; alpha-beta peptide; endocytosis; immunoglobulin

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Hetényi, A.; Szabó, E.; Imre, N.; Bhaumik, K.N.; Tököli, A.; Füzesi, T.; Hollandi, R.; Horvath, P.; Czibula, Á.; Monostori, É.; Deli, M.A.; Martinek, T.A. α/β-Peptides as Nanomolar Triggers of Lipid Raft-Mediated Endocytosis through GM1 Ganglioside Recognition. Pharmaceutics 2022, 14, 580. https://doi.org/10.3390/pharmaceutics14030580

AMA Style

Hetényi A, Szabó E, Imre N, Bhaumik KN, Tököli A, Füzesi T, Hollandi R, Horvath P, Czibula Á, Monostori É, Deli MA, Martinek TA. α/β-Peptides as Nanomolar Triggers of Lipid Raft-Mediated Endocytosis through GM1 Ganglioside Recognition. Pharmaceutics. 2022; 14(3):580. https://doi.org/10.3390/pharmaceutics14030580

Chicago/Turabian Style

Hetényi, Anasztázia, Enikő Szabó, Norbert Imre, Kaushik Nath Bhaumik, Attila Tököli, Tamás Füzesi, Réka Hollandi, Peter Horvath, Ágnes Czibula, Éva Monostori, Mária A. Deli, and Tamás A. Martinek. 2022. "α/β-Peptides as Nanomolar Triggers of Lipid Raft-Mediated Endocytosis through GM1 Ganglioside Recognition" Pharmaceutics 14, no. 3: 580. https://doi.org/10.3390/pharmaceutics14030580

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α/β-Peptides as Nanomolar Triggers of Lipid Raft-Mediated Endocytosis through GM1 Ganglioside Recognition

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