Next Article in Journal
Heme Oxygenase-1 Exerts Antiviral Activity against Hepatitis A Virus In Vitro
Next Article in Special Issue
The Metabolic Response of Various Cell Lines to Microtubule-Driven Uptake of Lipid- and Polymer-Coated Layer-by-Layer Microcarriers
Previous Article in Journal
Electrospun Nanofibers of Polycaprolactone/Collagen as a Sustained-Release Drug Delivery System for Artemisinin
Previous Article in Special Issue
Layer-by-Layer Nanoparticles of Tamoxifen and Resveratrol for Dual Drug Delivery System and Potential Triple-Negative Breast Cancer Treatment
Article

Revisiting the Cytotoxicity of Cationic Polyelectrolytes as a Principal Component in Layer-by-Layer Assembly Fabrication

Institute of Fundamental Medicine and Biology, Kazan Federal University, Kreml uramı, 18, 420008 Kazan, Republic of Tatarstan, Russia
*
Authors to whom correspondence should be addressed.
Academic Editors: Melgardt de Villiers and Daniel P. Otto
Pharmaceutics 2021, 13(8), 1230; https://doi.org/10.3390/pharmaceutics13081230
Received: 5 July 2021 / Revised: 2 August 2021 / Accepted: 5 August 2021 / Published: 9 August 2021
Polycations are an essential part of layer-by-layer (LbL)-assembled drug delivery systems, especially for gene delivery. In addition, they are used for other related applications, such as cell surface engineering. As a result, an assessment of the cytotoxicity of polycations and elucidation of the mechanisms of polycation toxicity is of paramount importance. In this study, we examined in detail the effects of a variety of water-soluble, positively charged synthetic polyelectrolytes on in vitro cytotoxicity, cell and nucleus morphology, and monolayer expansion changes. We have ranked the most popular cationic polyelectrolytes from the safest to the most toxic in relation to cell cultures. 3D cellular cluster formation was disturbed by addition of polyelectrolytes in most cases in a dose-dependent manner. Atomic force microscopy allowed us to visualize in detail the structures of the polyelectrolyte–DNA complexes formed due to electrostatic interactions. Our results indicate a relationship between the structure of the polyelectrolytes and their toxicity, which is necessary for optimization of drug and gene delivery systems. View Full-Text
Keywords: polyelectrolytes; polycations; layer-by-layer; cytotoxicity polyelectrolytes; polycations; layer-by-layer; cytotoxicity
Show Figures

Figure 1

MDPI and ACS Style

Naumenko, E.; Akhatova, F.; Rozhina, E.; Fakhrullin, R. Revisiting the Cytotoxicity of Cationic Polyelectrolytes as a Principal Component in Layer-by-Layer Assembly Fabrication. Pharmaceutics 2021, 13, 1230. https://doi.org/10.3390/pharmaceutics13081230

AMA Style

Naumenko E, Akhatova F, Rozhina E, Fakhrullin R. Revisiting the Cytotoxicity of Cationic Polyelectrolytes as a Principal Component in Layer-by-Layer Assembly Fabrication. Pharmaceutics. 2021; 13(8):1230. https://doi.org/10.3390/pharmaceutics13081230

Chicago/Turabian Style

Naumenko, Ekaterina, Farida Akhatova, Elvira Rozhina, and Rawil Fakhrullin. 2021. "Revisiting the Cytotoxicity of Cationic Polyelectrolytes as a Principal Component in Layer-by-Layer Assembly Fabrication" Pharmaceutics 13, no. 8: 1230. https://doi.org/10.3390/pharmaceutics13081230

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop