Next Article in Journal
The Vaginal Microbiota, Bacterial Biofilms and Polymeric Drug-Releasing Vaginal Rings
Next Article in Special Issue
Pharmacokinetics and Bioavailability of Carprofen in Rainbow Trout (Oncorhynchus mykiss) Broodstock
Previous Article in Journal
Next Step in Gene Delivery: Modern Approaches and Further Perspectives of AAV Tropism Modification
Previous Article in Special Issue
Evaluation of Bacterial Nanocellulose Membranes Loaded or Not with Nisin as a Complementary Treatment in Surgical Dehorning Wounds in Bovines
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Pharmaceutics
Volume 13
Issue 5
10.3390/pharmaceutics13050752
pharmaceutics-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Toxoplasma gondii Proteasome Subunit Alpha Type 1 with Chitosan: A Promising Alternative to Traditional Adjuvant

by
Zhengqing Yu
1,
Wenxi Ding
1,
Muhammad Tahir Aleem
1,
Junzhi Su
1,
Junlong Liu
2,
Jianxun Luo
2,
Ruofeng Yan
1,
Lixin Xu
1,
Xiaokai Song
1 and
Xiangrui Li
1,*
1
MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210000, China
2
State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China
*
Author to whom correspondence should be addressed.
Pharmaceutics 2021, 13(5), 752; https://doi.org/10.3390/pharmaceutics13050752
Submission received: 22 April 2021 / Revised: 12 May 2021 / Accepted: 18 May 2021 / Published: 19 May 2021
(This article belongs to the Special Issue Nanobiotechnology Systems-Based Veterinary Formulations)
Browse Figures
Review Reports Versions Notes

Abstract

As an important zoonotic protozoan, Toxoplasma gondii (T. gondii) has spread around the world, leading to infections in one-third of the population. There is still no effective vaccine or medicine against T. gondii, and recombinant antigens entrapped within nanospheres have benefits over traditional vaccines. In the present study, we first expressed and purified T. gondii proteasome subunit alpha type 1 (TgPSA1), then encapsulated the recombinant TgPSA1 (rTgPSA1) in chitosan nanospheres (CS nanospheres, rTgPSA1/CS nanospheres) and incomplete Freund’s adjuvant (IFA, rTgPSA1/IFA emulsion). Antigens entrapped in CS nanospheres reached an encapsulation efficiency of 67.39%, and rTgPSA1/CS nanospheres showed a more stable release profile compared to rTgPSA1/IFA emulsion in vitro. In vivo, Th1-biased cellular and humoral immune responses were induced in mice and chickens immunized with rTgPSA1/CS nanospheres and rTgPSA1/IFA emulsion, accompanied by promoted production of antibodies, IFN-γ, IL-4, and IL-17, and modulated production of IL-10. Immunization with rTgPSA1/CS nanospheres and rTgPSA1/IFA emulsion conferred significant protection, with prolonged survival time in mice and significantly decreased parasite burden in chickens. Furthermore, our results also indicate that rTgPSA1/CS nanospheres could be used as a substitute for rTgPSA1/IFA emulsion, with the optimal administration route being intramuscular in mass vaccination. Collectively, the results of this study indicate that rTgPSA1/CS nanospheres represent a promising vaccine to protect animals against acute toxoplasmosis.
Keywords: Toxoplasma gondii; proteasome subunit alpha type 1; incomplete Freund’s adjuvant; chitosan; immune protection; mouse; chicken Toxoplasma gondii; proteasome subunit alpha type 1; incomplete Freund’s adjuvant; chitosan; immune protection; mouse; chicken
Graphical Abstract

Share and Cite

MDPI and ACS Style

Yu, Z.; Ding, W.; Aleem, M.T.; Su, J.; Liu, J.; Luo, J.; Yan, R.; Xu, L.; Song, X.; Li, X. Toxoplasma gondii Proteasome Subunit Alpha Type 1 with Chitosan: A Promising Alternative to Traditional Adjuvant. Pharmaceutics 2021, 13, 752. https://doi.org/10.3390/pharmaceutics13050752

AMA Style

Yu Z, Ding W, Aleem MT, Su J, Liu J, Luo J, Yan R, Xu L, Song X, Li X. Toxoplasma gondii Proteasome Subunit Alpha Type 1 with Chitosan: A Promising Alternative to Traditional Adjuvant. Pharmaceutics. 2021; 13(5):752. https://doi.org/10.3390/pharmaceutics13050752

Chicago/Turabian Style

Yu, Zhengqing, Wenxi Ding, Muhammad Tahir Aleem, Junzhi Su, Junlong Liu, Jianxun Luo, Ruofeng Yan, Lixin Xu, Xiaokai Song, and Xiangrui Li. 2021. "Toxoplasma gondii Proteasome Subunit Alpha Type 1 with Chitosan: A Promising Alternative to Traditional Adjuvant" Pharmaceutics 13, no. 5: 752. https://doi.org/10.3390/pharmaceutics13050752

APA Style

Yu, Z., Ding, W., Aleem, M. T., Su, J., Liu, J., Luo, J., Yan, R., Xu, L., Song, X., & Li, X. (2021). Toxoplasma gondii Proteasome Subunit Alpha Type 1 with Chitosan: A Promising Alternative to Traditional Adjuvant. Pharmaceutics, 13(5), 752. https://doi.org/10.3390/pharmaceutics13050752

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop