Targeting Beclin1 as an Adjunctive Therapy against HIV Using Mannosylated Polyethylenimine Nanoparticles
Abstract
1. Introduction
2. Materials and Methods
2.1. Cell Culture and HIV Infection
2.2. Transfection of siRNA into Microglia, Astrocytes, and Neurons
2.3. Immunochemistry
2.4. Time-Lapse Assessment of Neuronal Viability
2.5. Viability Assay
2.6. Animals
2.7. Intranasal Administration of siRNA-PEI Nanocomplex into C57BL/6 Mice
2.8. Reverse Transcription Polymerase Chain Reaction (RT-PCR)
2.9. Nissl Staining
2.10. Enzyme-Linked Immunosorbent Assay (ELISA)
2.11. Liposome Composition and In Vivo Imaging System (IVIS)
2.12. Statistical Analysis
3. Results
3.1. siBeclin1 Reduces Viral Production in HIV-Infected Human Microglia Co-Administered with Antiretroviral Drugs and Attenuates Secreted Viral-Induced Inflammatory Molecules in Human Microglia and Human Astrocytes
3.2. PEI-Man Targets Human Microglia and Human Astrocytes-Expressing Mannose Membrane Receptors and Does Not Exert Toxicity to Human Neuronal Cells
3.3. Biodistribution of the PEI-Man-siBeclin1 Nanoparticle in C57BL/6 Mice Brains after Intranasal Delivery
3.4. PEI-Man-siBeclin1 Nanoparticle Delivered to the Brain Causes Minimal Toxicity and Reduces the Secretion of Inflammatory Molecules
3.5. Biodistribution of DIR-Liposome-Nanoparticles in C57BL/6 Mice after Intranasal Delivery
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Rodriguez, M.; Soler, Y.; Muthu Karuppan, M.K.; Zhao, Y.; Batrakova, E.V.; El-Hage, N. Targeting Beclin1 as an Adjunctive Therapy against HIV Using Mannosylated Polyethylenimine Nanoparticles. Pharmaceutics 2021, 13, 223. https://doi.org/10.3390/pharmaceutics13020223
Rodriguez M, Soler Y, Muthu Karuppan MK, Zhao Y, Batrakova EV, El-Hage N. Targeting Beclin1 as an Adjunctive Therapy against HIV Using Mannosylated Polyethylenimine Nanoparticles. Pharmaceutics. 2021; 13(2):223. https://doi.org/10.3390/pharmaceutics13020223
Chicago/Turabian StyleRodriguez, Myosotys, Yemmy Soler, Mohan Kumar Muthu Karuppan, Yuling Zhao, Elena V. Batrakova, and Nazira El-Hage. 2021. "Targeting Beclin1 as an Adjunctive Therapy against HIV Using Mannosylated Polyethylenimine Nanoparticles" Pharmaceutics 13, no. 2: 223. https://doi.org/10.3390/pharmaceutics13020223
APA StyleRodriguez, M., Soler, Y., Muthu Karuppan, M. K., Zhao, Y., Batrakova, E. V., & El-Hage, N. (2021). Targeting Beclin1 as an Adjunctive Therapy against HIV Using Mannosylated Polyethylenimine Nanoparticles. Pharmaceutics, 13(2), 223. https://doi.org/10.3390/pharmaceutics13020223