Next Article in Journal
A Comprehensive Review about the Molecular Structure of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2): Insights into Natural Products against COVID-19
Next Article in Special Issue
3D-Printed Products for Topical Skin Applications: From Personalized Dressings to Drug Delivery
Previous Article in Journal
Advances of Nanomedicine in Radiotherapy
Previous Article in Special Issue
Human Skin Permeation Enhancement Using PLGA Nanoparticles Is Mediated by Local pH Changes
Article

Use of an In Vitro Skin Parallel Artificial Membrane Assay (Skin-PAMPA) as a Screening Tool to Compare Transdermal Permeability of Model Compound 4-Phenylethyl-Resorcinol Dissolved in Different Solvents

1
Department of Pharmaceutical Chemistry, Semmelweis University, H-1092 Budapest, Hungary
2
Pion Inc., Billerica, MA 01821, USA
3
Department of Organic Chemistry and Technology, Budapest University of Technology and Economics, H-1111 Budapest, Hungary
4
L’Oréal Research & Innovation, 93601 Aulnay-sous Bois, France
*
Author to whom correspondence should be addressed.
Academic Editors: Ana Melero, Ruy Carlos Ruver Beck and Heather Benson
Pharmaceutics 2021, 13(11), 1758; https://doi.org/10.3390/pharmaceutics13111758
Received: 7 September 2021 / Revised: 6 October 2021 / Accepted: 18 October 2021 / Published: 21 October 2021
(This article belongs to the Special Issue Skin Drug Delivery: Local and Systemic Applications)
Absorption through the skin of topically applied chemicals is relevant for both formulation development and safety assessment, especially in the early stages of development. However, the supply of human skin is limited, and the traditional in vitro methods are of low throughput. As an alternative, an artificial membrane-based Skin Parallel Artificial Membrane Permeability Assay (Skin-PAMPA) has been developed to mimic the permeability through the stratum corneum. In this study, this assay was used to measure the permeability of a model compound, 4-phenylethyl-resorcinol (PER), dissolved in 13 different solvents that are commonly used in cosmetic formulation development. The study was performed at concentrations close to the saturated solution of PER in each solvent to investigate the maximum thermodynamic potential of the solvents. The permeability of PER in selected solvents was also measured on ex vivo pig skin for comparison. Pig ear skin is an accepted alternative model of human skin. The permeability coefficient, which is independent of the concentration of the applied solution, showed a good correlation (R2 = 0.844) between the Skin-PAMPA and the pig skin permeation data. Our results support the use of the Skin-PAMPA to screen the suitability of different solvents for non-polar compounds at an early stage of formulation development. View Full-Text
Keywords: Skin-PAMPA; formulation; skin barrier; permeability; pig skin; safety testing Skin-PAMPA; formulation; skin barrier; permeability; pig skin; safety testing
Show Figures

Figure 1

MDPI and ACS Style

Sinkó, B.; Bárdos, V.; Vesztergombi, D.; Kádár, S.; Malcsiner, P.; Moustie, A.; Jouy, C.; Takács-Novák, K.; Grégoire, S. Use of an In Vitro Skin Parallel Artificial Membrane Assay (Skin-PAMPA) as a Screening Tool to Compare Transdermal Permeability of Model Compound 4-Phenylethyl-Resorcinol Dissolved in Different Solvents. Pharmaceutics 2021, 13, 1758. https://doi.org/10.3390/pharmaceutics13111758

AMA Style

Sinkó B, Bárdos V, Vesztergombi D, Kádár S, Malcsiner P, Moustie A, Jouy C, Takács-Novák K, Grégoire S. Use of an In Vitro Skin Parallel Artificial Membrane Assay (Skin-PAMPA) as a Screening Tool to Compare Transdermal Permeability of Model Compound 4-Phenylethyl-Resorcinol Dissolved in Different Solvents. Pharmaceutics. 2021; 13(11):1758. https://doi.org/10.3390/pharmaceutics13111758

Chicago/Turabian Style

Sinkó, Bálint, Vivien Bárdos, Dániel Vesztergombi, Szabina Kádár, Petra Malcsiner, Anne Moustie, Chantal Jouy, Krisztina Takács-Novák, and Sebastien Grégoire. 2021. "Use of an In Vitro Skin Parallel Artificial Membrane Assay (Skin-PAMPA) as a Screening Tool to Compare Transdermal Permeability of Model Compound 4-Phenylethyl-Resorcinol Dissolved in Different Solvents" Pharmaceutics 13, no. 11: 1758. https://doi.org/10.3390/pharmaceutics13111758

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop