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Article

BSA- and Elastin-Coated GO, but Not Collagen-Coated GO, Enhance the Biological Performance of Alginate Hydrogels

1
NanoBioCel Group, Laboratory of Pharmacy and Pharmaceutical Technology, Faculty of Pharmacy, University of the Basque Country UPV/EHU, 01006 Vitoria-Gasteiz, Spain
2
Biomedical Research Networking Center in Bioengineering, Biomaterials, and Nanomedicine, CIBER-BBN, 28029 Madrid, Spain
3
Department of Organic Chemistry I, Faculty of Pharmacy and Lascaray Research Center, University of the Basque Country (UPV/EHU), Paseo de la Universidad 7, 01006 Vitoria, Spain
4
Instituto de Ciencia de Materiales de Aragón (Universidad de Zaragoza-CSIC), Facultad de Ciencias, 50009 Zaragoza, Spain
5
R&D Human Health, Bioibérica S.A.U., 08029 Barcelona E-, Spain
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Pharmaceutics 2020, 12(6), 543; https://doi.org/10.3390/pharmaceutics12060543
Received: 20 May 2020 / Revised: 5 June 2020 / Accepted: 8 June 2020 / Published: 11 June 2020
(This article belongs to the Section Drug Delivery and Controlled Release)
The use of embedded cells within alginate matrices is a developing technique with great clinical applications in cell-based therapies. However, one feature that needs additional investigation is the improvement of alginate-cells viability, which could be achieved by integrating other materials with alginate to improve its surface properties. In recent years, the field of nanotechnology has shown the many properties of a huge number of materials. Graphene oxide (GO), for instance, seems to be a good choice for improving alginate cell viability and functionality. We previously observed that GO, coated with fetal bovine serum (FBS) within alginate hydrogels, improves the viability of embedded myoblasts. In the current research, we aim to study several proteins, specifically bovine serum albumin (BSA), type I collagen and elastin, to discern their impact on the previously observed improvement on embedded myoblasts within alginate hydrogels containing GO coated with FBS. Thus, we describe the mechanisms of the formation of BSA, collagen and elastin protein layers on the GO surface, showing a high adsorption by BSA and elastin, and a decreasing GO impedance and capacitance. Moreover, we described a better cell viability and protein release from embedded cells within hydrogels containing protein-coated GO. We conclude that these hybrid hydrogels could provide a step forward in regenerative medicine. View Full-Text
Keywords: graphene oxide; bovine serum albumin; type I collagen; elastin; alginate hydrogels; cell viability graphene oxide; bovine serum albumin; type I collagen; elastin; alginate hydrogels; cell viability
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MDPI and ACS Style

Raslan, A.; Saenz del Burgo, L.; Espona-Noguera, A.; Ochoa de Retana, A.M.; Sanjuán, M.L.; Cañibano-Hernández, A.; Gálvez-Martín, P.; Ciriza, J.; Pedraz, J.L. BSA- and Elastin-Coated GO, but Not Collagen-Coated GO, Enhance the Biological Performance of Alginate Hydrogels. Pharmaceutics 2020, 12, 543. https://doi.org/10.3390/pharmaceutics12060543

AMA Style

Raslan A, Saenz del Burgo L, Espona-Noguera A, Ochoa de Retana AM, Sanjuán ML, Cañibano-Hernández A, Gálvez-Martín P, Ciriza J, Pedraz JL. BSA- and Elastin-Coated GO, but Not Collagen-Coated GO, Enhance the Biological Performance of Alginate Hydrogels. Pharmaceutics. 2020; 12(6):543. https://doi.org/10.3390/pharmaceutics12060543

Chicago/Turabian Style

Raslan, Ahmed, Laura Saenz del Burgo, Albert Espona-Noguera, Ana María Ochoa de Retana, María Luisa Sanjuán, Alberto Cañibano-Hernández, Patricia Gálvez-Martín, Jesús Ciriza, and Jose Luis Pedraz. 2020. "BSA- and Elastin-Coated GO, but Not Collagen-Coated GO, Enhance the Biological Performance of Alginate Hydrogels" Pharmaceutics 12, no. 6: 543. https://doi.org/10.3390/pharmaceutics12060543

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