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Article

A Novel Composite Hydrogel Composed of Formic Acid-Decellularized Pepsin-Soluble Extracellular Matrix Hydrogel and Sacchachitin Hydrogel as Wound Dressing to Synergistically Accelerate Diabetic Wound Healing

1
School of Pharmacy, College of Pharmacy, Taipei Medical University, 250 Wu-Hsing Street, Taipei 11031, Taiwan
2
Department of Surgery, School of Medicine, College of Medicine, Taipei Medical University, 250 Wu-Hsing Street, Taipei 11031, Taiwan
3
Division of General Surgery, Department of Surgery, Taipei Medical University Hospital, 252 Wu-Hsing Street, Taipei 11031, Taiwan
*
Authors to whom correspondence should be addressed.
Pharmaceutics 2020, 12(6), 538; https://doi.org/10.3390/pharmaceutics12060538
Received: 29 April 2020 / Revised: 6 June 2020 / Accepted: 9 June 2020 / Published: 11 June 2020
(This article belongs to the Special Issue Advances in Wound Dressings and Materials)
Extracellular matrix (ECM) hydrogel can create a favorable regenerative microenvironment and act as a promising dressing for accelerating the healing of diabetic wound. In this study, a simple and effective decellularization technique was developed and optimized to obtain acellular extracellular matrix (aECM) from porcine skin. It was found that decellularization at 30% formic acid for 72 h effectively decellularized porcine skin while retaining >75% collagen and ~37% GAG in the aECM with no presence of nuclei of cellular remnants. aECM hydrogel was fabricated by digesting aECM with pepsin in various acidic solutions (0.1 N HCl, glycolic acid (GA) and 2-pyrrolidone-5-carboxylic acid (PCA)) and then treated with a pH-controlled neutralization and temperature-controlled gelation procedure. Based on physical characterizations, including SDS-PAGE, rheological analysis and SEM analysis, aECMHCl hydrogels fabricated at 25 mg/mL in 0.1 N HCl were selected. Four polymeric ECM-mimic hydrogels, including sacchachitin (SC), hyaluronic acid (HA) and chitosan (CS) and three composite hydrogels of combining SC either with aECMHCl,25 (aECMHCl/SC), HA (HA/SC) or CS (SC/CS) were prepared and evaluated for WS-1 cell viability and wound-healing effectiveness. Cell viability study confirmed that no hydrogel dressings possessed any toxicity at all concentrations examined and ECMHCl, HA and ECMHCl/SC at higher concentrations (>0.05%) induced statistically significant proliferation. Diabetic wound healing study and histological examinations revealed that ECMHCl/SC hydrogel was observed to synergistically accelerate wound healing and ultimately stimulated the growth of hair follicles and sweat glands in the healing wound indicating the wound had healed as functional tissues. The results support the great potential of this newly produced ECMHCl/SC composite hydrogel for healing and regeneration of diabetic wounds. View Full-Text
Keywords: acellular extracellular matrix; porcine skin; sacchachitin; wound dressing; hydrogel acellular extracellular matrix; porcine skin; sacchachitin; wound dressing; hydrogel
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MDPI and ACS Style

Hsieh, C.-M.; Wang, W.; Chen, Y.-H.; Wei, P.-S.; Liu, Y.-H.; Sheu, M.-T.; Ho, H.-O. A Novel Composite Hydrogel Composed of Formic Acid-Decellularized Pepsin-Soluble Extracellular Matrix Hydrogel and Sacchachitin Hydrogel as Wound Dressing to Synergistically Accelerate Diabetic Wound Healing. Pharmaceutics 2020, 12, 538. https://doi.org/10.3390/pharmaceutics12060538

AMA Style

Hsieh C-M, Wang W, Chen Y-H, Wei P-S, Liu Y-H, Sheu M-T, Ho H-O. A Novel Composite Hydrogel Composed of Formic Acid-Decellularized Pepsin-Soluble Extracellular Matrix Hydrogel and Sacchachitin Hydrogel as Wound Dressing to Synergistically Accelerate Diabetic Wound Healing. Pharmaceutics. 2020; 12(6):538. https://doi.org/10.3390/pharmaceutics12060538

Chicago/Turabian Style

Hsieh, Chien-Ming, Weu Wang, Ying-Hsuan Chen, Pu-Sheng Wei, Yu-Hsuan Liu, Ming-Thau Sheu, and Hsiu-O Ho. 2020. "A Novel Composite Hydrogel Composed of Formic Acid-Decellularized Pepsin-Soluble Extracellular Matrix Hydrogel and Sacchachitin Hydrogel as Wound Dressing to Synergistically Accelerate Diabetic Wound Healing" Pharmaceutics 12, no. 6: 538. https://doi.org/10.3390/pharmaceutics12060538

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