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Article

In Vitro Evaluation of Antimicrobial Activity of Minocycline Formulations for Topical Application in Periodontal Therapy

1
Laboratory of Oral Microbiology, Department of Periodontology, School of Dental Medicine, University of Bern, CH-3010 Bern, Switzerland
2
Institute of Pharmacy, Martin-Luther University Halle, D-06120 Halle (Saale), Germany
3
Characterization of Medical and Cosmetic Care Products, Fraunhofer Institute for Microstructures and Materials IMWS, D-06120 Halle/Saale, Germany
4
Department of Periodontology, School of Dental Medicine, University of Bern, CH-3010 Bern, Switzerland
5
Drug Design and Target Validation, Fraunhofer Institute for Cell Therapy and Immunology IZI-MWT and PerioTrap Pharmaceuticals GmbH, D-06120 Halle/Saale, Germany
*
Author to whom correspondence should be addressed.
Pharmaceutics 2020, 12(4), 352; https://doi.org/10.3390/pharmaceutics12040352
Received: 20 March 2020 / Revised: 9 April 2020 / Accepted: 11 April 2020 / Published: 13 April 2020
(This article belongs to the Special Issue Controlled Delivery Formulations)
Periodontal therapy using antimicrobials that are topically applied requires slow or controlled release devices. The in vitro antimicrobial activity of biodegradable polymer formulations that contain a new minocycline lipid complex (P-MLC) was evaluated. The new P-MLC formulations that contained 11.5% minocycline were compared with pure minocycline or an existing commercial formulation, which included determination of minimal inhibitory concentration (MIC) values against two oral bacteria and activity on six-species periodontal biofilm. Moreover, the flow of gingival crevicular fluid (GCF) was modeled up to 42 days and the obtained eluates were tested both for MIC values and inhibiting biofilm formation. In general, MICs of the P-MLC formulations were slightly increased as compared with pure minocycline. Biofilm formation was clearly inhibited by all tested formulations containing minocycline with no clear difference between them. In 3.5 day old biofilms, all formulations with 250 µg/mL minocycline decreased bacterial counts by 3 log10 and metabolic activity with no difference to pure antimicrobials. Eluates of experimental formulations showed superiority in antimicrobial activity. Eluates of one experimental formulation (P503-MLC) still inhibited biofilm formation at 28 days, with a reduction by 1.87 log10 colony forming units (CFU) vs. the untreated control. The new experimental formulations can easily be instilled in periodontal pockets and represent alternatives in local antimicrobials, and thus warrant further testing. View Full-Text
Keywords: controlled release; periodontitis; local antibiotics; in vitro model; gingival flow controlled release; periodontitis; local antibiotics; in vitro model; gingival flow
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MDPI and ACS Style

Schmid, J.-L.; Kirchberg, M.; Sarembe, S.; Kiesow, A.; Sculean, A.; Mäder, K.; Buchholz, M.; Eick, S. In Vitro Evaluation of Antimicrobial Activity of Minocycline Formulations for Topical Application in Periodontal Therapy. Pharmaceutics 2020, 12, 352. https://doi.org/10.3390/pharmaceutics12040352

AMA Style

Schmid J-L, Kirchberg M, Sarembe S, Kiesow A, Sculean A, Mäder K, Buchholz M, Eick S. In Vitro Evaluation of Antimicrobial Activity of Minocycline Formulations for Topical Application in Periodontal Therapy. Pharmaceutics. 2020; 12(4):352. https://doi.org/10.3390/pharmaceutics12040352

Chicago/Turabian Style

Schmid, Jan-Luca, Martin Kirchberg, Sandra Sarembe, Andreas Kiesow, Anton Sculean, Karsten Mäder, Mirko Buchholz, and Sigrun Eick. 2020. "In Vitro Evaluation of Antimicrobial Activity of Minocycline Formulations for Topical Application in Periodontal Therapy" Pharmaceutics 12, no. 4: 352. https://doi.org/10.3390/pharmaceutics12040352

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