Light-Activatable Transfection System Using Hybrid Vectors Composed of Thermosensitive Dendron Lipids and Gold Nanorods
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Preparation of MUA-AuNR
2.3. Preparation of Hybrid Vector and Complex
2.4. General Characterization
2.5. Agarose Gel Electrophoresis
2.6. Transfection
2.7. Cellular Association of Complexes
2.8. Intracellular Distribution Analysis
2.9. Statistical Analysis
3. Results
3.1. Characterization of Hybrid Vectors and Complexes
3.2. Transfection
3.3. Intracellular Behavior
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Sample | Temperature (°C) | Particle Size (nm) | Zeta Potential (mV) |
---|---|---|---|
MUA-AuNR | 25 | 36 ± 1 | −23.5 ± 1.7 |
DL suspension | 25 | 724 ± 73 | 9.19 ± 0.2 |
50 | 2565 ± 248 | – | |
Hybrid vector | 25 | 135 ± 12 | 4.19 ± 0.4 |
50 | 297 ± 19 | – | |
Complex | 25 | 185 ± 50 | 3.66 ± 0.8 |
50 | 300 ± 21 | – |
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Hashimoto, T.; Hirata, T.; Yuba, E.; Harada, A.; Kono, K. Light-Activatable Transfection System Using Hybrid Vectors Composed of Thermosensitive Dendron Lipids and Gold Nanorods. Pharmaceutics 2020, 12, 239. https://doi.org/10.3390/pharmaceutics12030239
Hashimoto T, Hirata T, Yuba E, Harada A, Kono K. Light-Activatable Transfection System Using Hybrid Vectors Composed of Thermosensitive Dendron Lipids and Gold Nanorods. Pharmaceutics. 2020; 12(3):239. https://doi.org/10.3390/pharmaceutics12030239
Chicago/Turabian StyleHashimoto, Takuya, Tomoya Hirata, Eiji Yuba, Atsushi Harada, and Kenji Kono. 2020. "Light-Activatable Transfection System Using Hybrid Vectors Composed of Thermosensitive Dendron Lipids and Gold Nanorods" Pharmaceutics 12, no. 3: 239. https://doi.org/10.3390/pharmaceutics12030239