Sustained Calcium(II)-Release to Impart Bioactivity in Hybrid Glass Scaffolds for Bone Tissue Engineering
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Methods
2.2.1. Template Fabrication
2.2.2. Silica Sol Synthesis
2.2.3. Cross-Linker Synthesis
2.2.4. Hybrid Scaffold Production
2.2.5. Hybrid Scaffold Production with Incorporation of Bioactive Glass (BG) Microparticles
2.2.6. Scaffold Characterization
Scanning Electron Microscopy (SEM) and Energy Dispersive X-ray Spectroscopy (EDX)
Mechanical Testing
Characterization of Scaffold Degradation and Calcium(II) Release
Quantification of Released Calcium(II)
Bioactivity Testing in Simulated Body Fluid (SBF)
Cell Culture
2.2.7. Statistical Analysis
3. Results
3.1. TEP as Solvent for Hybrid Sols
3.2. Incorporation of Calcium(II) from CaCl2 and Bioactive Glass Microparticles (Vitryxx®)
3.3. Scaffold Strength
3.4. Scaffold Degradation upon Incubation in Aqueous Medium
3.5. Bioactivity Testing
3.6. Calcium Ion Release and Media pH
3.7. Cell Culture Experiments
4. Discussion
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Appendix A
Formulation | Weight Loss, wt% |
10C10P | 26.9 ± 0.3% |
5C10P | 18.4 ± 1.3% |
2.5C10P | 14.4 ± 0.5% |
0C10P | 12.1 ± 0.3% |
0C10P + BG-MP | 11.3 ± 0.5% |
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Sol Formulation | TEOS | Calcium(II) | TEP |
---|---|---|---|
0C10P | 90 | 0 | 10 |
2.5C10P | 87.5 | 2.5 | 10 |
5C10P | 85 | 5 | 10 |
10C10P | 80 | 10 | 10 |
Step | |
---|---|
1 | heating from room temperature to 60 °C, dwell at 60 °C for 96 h |
2 | heating from 60 °C to 90 °C, dwell at 90 °C for 24 h |
3 | heating from 90 °C to 130 °C, dwell at 130 °C for 48 h |
Ca(II) Release, [%/d] | ||||
---|---|---|---|---|
Incubation Time, [d] | 10C10P | 5C10P | 2.5C10P | 1C10P |
0–1 | 98.37 ± 3.88 | 85.87 ± 3.89 | 72.36 ± 1.61 | 74.02 ± 3.47 |
1–3 | 0.09 ± 0.62 | 1.58 ± 1.59 | 4.65 ± 1.93 | 5.73 ± 0.45 |
3–7 | 0.30 ± 0.04 | 1.43 ± 0.18 | 2.09 ± 0.29 | 2.32 ± 0.05 |
7–14 | 0.04 ± 0.02 | 0.45 ± 0.09 | 0.73 ± 0.08 | 0.37 ± 0.05 |
14–21 | 0.00 | 0.13 ± 0.05 | 0.24 ± 0.09 | 0.36 ± 0.14 |
21–28 | 0.00 | 0.09 ± 0.04 | 0.15 ± 0.04 | 0.02 ± 0.04 |
A | Calcium(II) Release [µg/5mL/d] | |||||
---|---|---|---|---|---|---|
time, [d] | 7 mg BG-MP | 0C10P + 7 mg BG-MP | 2.5C10P + 14 mg BG‑MP | 2.5C10P + 17 mg BG-MP | (2.5C10P + 14 mg BG-MP)-2.5C10P | (2.5C10P + 17 mg BG-MP)-2.5C10P |
0–1 | 459.4 ± 110.1 | 11.9 ± 6.2 | 1003.1 ± 35.6 | 1023.1 ± 37.3 | 81.1 ± 11.6 | 77.5 ± 8.5 |
1–3 | 74.3 ± 36.5 | 14.3 ± 1.8 | 220.7 ± 14.6 | 186.0 ± 19.6 | 78.5 ± 43.2 | 55.5 ± 24.0 |
3–7 | 2.8 ± 1.0 | 14.6 ± 2.50 | 84.8 ± 21.7 | 103.7 ± 7.4 | 53.9 ± 24.3 | 72.8 ± 6.7 |
7–14 | 2.0 ± 1.3 | 10.7 ± 1.2 | 56.8 ± 2.4 | 71.9 ± 5.1 | 46.1 ± 2.1 | 61.1 ± 4.6 |
14–21 | 0.9 ± 0.9 | 9.3 ± 1.1 | 48.1 ± 1.0 | 54.7 ± 3.6 | 44.5 ± 1.6 | 51.1 ± 3.8 |
21–28 | 0.3 ± 0.5 | 10.4 ± 1.5 | 38.0 ± 2.7 | 48.3 ± 6.7 | 35.9 ± 3.1 | 46.2 ± 6.6 |
B | Formulation | Average Calcium(II) Release Rate [µg/5mL/d] | ||||
0C10P + 7 mg BG-MP | 11.9 | |||||
(2.5C10P + 14 mg BG-MP)–2.5C10P | 42.1 * | |||||
(2.5C10P + 17 mg BG-MP)–2.5C10P | 52.8 * |
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Kuzmenka, D.; Sewohl, C.; König, A.; Flath, T.; Hahnel, S.; Schulze, F.P.; Hacker, M.C.; Schulz-Siegmund, M. Sustained Calcium(II)-Release to Impart Bioactivity in Hybrid Glass Scaffolds for Bone Tissue Engineering. Pharmaceutics 2020, 12, 1192. https://doi.org/10.3390/pharmaceutics12121192
Kuzmenka D, Sewohl C, König A, Flath T, Hahnel S, Schulze FP, Hacker MC, Schulz-Siegmund M. Sustained Calcium(II)-Release to Impart Bioactivity in Hybrid Glass Scaffolds for Bone Tissue Engineering. Pharmaceutics. 2020; 12(12):1192. https://doi.org/10.3390/pharmaceutics12121192
Chicago/Turabian StyleKuzmenka, Dzmitry, Claudia Sewohl, Andreas König, Tobias Flath, Sebastian Hahnel, Fritz Peter Schulze, Michael C. Hacker, and Michaela Schulz-Siegmund. 2020. "Sustained Calcium(II)-Release to Impart Bioactivity in Hybrid Glass Scaffolds for Bone Tissue Engineering" Pharmaceutics 12, no. 12: 1192. https://doi.org/10.3390/pharmaceutics12121192