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Open AccessArticle

Preparation of Squalene Oil-Based Emulsion Adjuvants Employing a Self-Emulsifying Drug Delivery System and Assessment of Mycoplasma hyopneumoniae-Specific Antibody Titers in BALB/c Mice

1
College of Pharmacy, Keimyung University, 1095 Dalgubeol-daero, Dalseo-gu, Daegu 42601, Korea
2
Komipharm International Co., Ltd., 17 Gyeongje-ro, Siheung-si, Gyonggi-do 15094, Korea
*
Author to whom correspondence should be addressed.
The authors equally contributed to this work.
Pharmaceutics 2019, 11(12), 667; https://doi.org/10.3390/pharmaceutics11120667
Received: 31 October 2019 / Revised: 3 December 2019 / Accepted: 6 December 2019 / Published: 10 December 2019
(This article belongs to the Special Issue Discovery and Evaluation of Novel Adjuvants for Vaccine Formulations)
In this study, a self-emulsifying drug delivery system (SEDDS) was employed to prepare novel squalene oil-based emulsion adjuvants. Deionized water, 0.01% and 0.02% (w/v) carbomer solutions of C-971P NF and C-940 grades were used to prepare emulsions containing 3%, 5% and 10% of squalene oil. Altogether 15 candidate emulsions were prepared and used as adjuvants for the delivery of a combination vaccine containing a porcine circovirus type 2 (PCV2) antigen and inactivated Mycoplasma hyopneumoniae (J101 strain) antigen. Most of the emulsions showed droplet sizes in the submicron range and maintained zeta potential values between −40 mV to 0 mV for six months, indicating good physical stability as a vaccine adjuvant. Emulsion-based candidate adjuvants prepared with SEDDS technology stimulated IgG, IgG1 and IgG2a like a currently commercially available adjuvant, Montanide ISATM 201, and they were safe and their Mycoplasma hyopneumoniae-specific antibody titers were considered as comparable with that of Montanide ISATM 201. View Full-Text
Keywords: self-emulsifying drug delivery system; squalene oil; emulsion adjuvants; Mycoplasma hyopneumoniae-specific antibody titers self-emulsifying drug delivery system; squalene oil; emulsion adjuvants; Mycoplasma hyopneumoniae-specific antibody titers
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MDPI and ACS Style

Bastola, R.; Seo, J.-E.; Keum, T.; Noh, G.; Choi, J.W.; Shin, J.I.; Kim, J.H.; Lee, S. Preparation of Squalene Oil-Based Emulsion Adjuvants Employing a Self-Emulsifying Drug Delivery System and Assessment of Mycoplasma hyopneumoniae-Specific Antibody Titers in BALB/c Mice. Pharmaceutics 2019, 11, 667.

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