Co-Encapsulation of Chlorin e6 and Chemotherapeutic Drugs in a PEGylated Liposome Enhance the Efficacy of Tumor Treatment: Pharmacokinetics and Therapeutic Efficacy
Abstract
:1. Introduction
2. Materials and Methods
2.1. Chemicals
2.2. Cell Cultures
2.3. Preparation and Characterization of Dual-Effect Liposome
2.4. Stability Analysis and In Vitro Drug Release Profile of Dual-Effect Liposome
2.5. Animal Experiments in Mice
2.5.1. Biodistribution and Pharmacokinetic Studies of Pl-Dox-Ce6 & Pl-Cddp-Ce6 in C26 Tumor-Bearing Balb/c Mice
2.5.2. Tumor Drug Deposition Determination
2.5.3. In Vivo Therapeutic Experiments
2.6. Statistics
3. Results
3.1. Biodistribution and Therapeutic Efficacy of PL-Dox-Ce6
3.2. The Impact of the Irradiation Scheme on Drug Accumulation in Tumor
3.3. Irradiation Scheme on the Therapeutic Efficiency
3.4. Treatment for a Larger Tumor Model
3.5. The Release Profile of Bioavailable Drug after Light Irradiation
3.6. Characteristics and Stability of PL-cDDP-Ce6 Liposomes
3.7. The Cytotoxicity of PL-cDDP-Ce6 In Vitro and In Vivo
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Peng, P.-C.; Hong, R.-L.; Tsai, T.; Chen, C.-T. Co-Encapsulation of Chlorin e6 and Chemotherapeutic Drugs in a PEGylated Liposome Enhance the Efficacy of Tumor Treatment: Pharmacokinetics and Therapeutic Efficacy. Pharmaceutics 2019, 11, 617. https://doi.org/10.3390/pharmaceutics11110617
Peng P-C, Hong R-L, Tsai T, Chen C-T. Co-Encapsulation of Chlorin e6 and Chemotherapeutic Drugs in a PEGylated Liposome Enhance the Efficacy of Tumor Treatment: Pharmacokinetics and Therapeutic Efficacy. Pharmaceutics. 2019; 11(11):617. https://doi.org/10.3390/pharmaceutics11110617
Chicago/Turabian StylePeng, Po-Chun, Ruey-Long Hong, Tsuimin Tsai, and Chin-Tin Chen. 2019. "Co-Encapsulation of Chlorin e6 and Chemotherapeutic Drugs in a PEGylated Liposome Enhance the Efficacy of Tumor Treatment: Pharmacokinetics and Therapeutic Efficacy" Pharmaceutics 11, no. 11: 617. https://doi.org/10.3390/pharmaceutics11110617