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Viruses 2017, 9(7), 166;

Systemic Propagation of a Fluorescent Infectious Clone of a Polerovirus Following Inoculation by Agrobacteria and Aphids

Université de Strasbourg, Institut National de la Recherche Agronomique, SVQV UMR-A 1131, 68000 Colmar, France
UMR 385 BGPI, Institut National de la Recherche Agronomique—Centre de Coopération Internationale en Recherche Agronomique pour le Développement, SupAgro, CIRAD TA-A54/K, Campus International de Baillarguet, 34398 Montpellier, France
Institut de Biologie Moléculaire des Plantes, Centre National de la Recherche Scientifique, UPR 2357, Université de Strasbourg, 12 rue du Général Zimmer, 67084 Strasbourg, France
Institute of Plant Science Paris Saclay (IPS2), CNRS, INRA, University Paris Diderot, University of Paris-Saclay, 91405 Orsay, France
Author to whom correspondence should be addressed.
Academic Editor: K. Andrew White
Received: 3 May 2017 / Revised: 16 June 2017 / Accepted: 22 June 2017 / Published: 29 June 2017
(This article belongs to the Section Viruses of Plants, Fungi and Protozoa)
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A fluorescent viral clone of the polerovirus Turnip yellows virus (TuYV) was engineered by introducing the Enhanced Green Fluorescent Protein (EGFP) sequence into the non-structural domain sequence of the readthrough protein, a minor capsid protein. The resulting recombinant virus, referred to as TuYV-RTGFP, was infectious in several plant species when delivered by agroinoculation and invaded efficiently non-inoculated leaves. As expected for poleroviruses, which infect only phloem cells, the fluorescence emitted by TuYV-RTGFP was restricted to the vasculature of infected plants. In addition, TuYV-RTGFP was aphid transmissible and enabled the observation of the initial sites of infection in the phloem after aphid probing in epidermal cells. The aphid-transmitted virus moved efficiently to leaves distant from the inoculation sites and importantly retained the EGFP sequence in the viral genome. This work reports on the first engineered member in the Luteoviridae family that can be visualized by fluorescence emission in systemic leaves of different plant species after agroinoculation or aphid transmission. View Full-Text
Keywords: polerovirus; fluorescent clone; systemic infection; aphid transmission polerovirus; fluorescent clone; systemic infection; aphid transmission

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Boissinot, S.; Pichon, E.; Sorin, C.; Piccini, C.; Scheidecker, D.; Ziegler-Graff, V.; Brault, V. Systemic Propagation of a Fluorescent Infectious Clone of a Polerovirus Following Inoculation by Agrobacteria and Aphids. Viruses 2017, 9, 166.

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