Microscopic Characterization of the Brazilian Giant Samba Virus
Abstract
:1. Introduction
2. Materials and Methods
2.1. Virus Preparation
2.2. Preparation of Cryo Specimens
2.3. Low-Dose Imaging Conditions
2.4. Cryo-Electron Tomography
2.5. Fluorescence Microscopy
2.6. Scanning Electron Microscopy
2.7. Capsid and Nucleocapsid Measurements
3. Results
3.1. Cryo-Electron Microscopy (Cryo-EM) Revealed the Size and Morphologies of SMBV Particles
3.2. A Comparison of APMV and SMBV Particles through the Use of Cryo-Electron Microscopy
3.3. Three-Dimensional Structural Information of the Entire SMBV Virion Was Obtained through the Use of Cryo-Electron Tomography (Cryo-ET)
3.4. A Comparison of SMBV and APMV Particles via Scanning Electron Microscopy (SEM) Revealed Differences in Capsid Regularity and Potential Viral Ultrastructure
3.5. Fluorescence Light Microscopy Revealed Biomolecular Composition and Ultrastructural Lattice Formation of SMBV and APMV Particles
4. Discussion
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
Abbreviations
SMBV | Samba Virus |
APMV | Acanthamoeba polyphaga mimivirus |
Cryo-EM | Cryo-electron Microscopy |
Cryo-ET | Cryo-electron Tomography |
SEM | Scanning Electron Microscopy |
TEM | Transmission Electron Microscopy |
RMC | Random Model Computation |
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Schrad, J.R.; Young, E.J.; Abrahão, J.S.; Cortines, J.R.; Parent, K.N. Microscopic Characterization of the Brazilian Giant Samba Virus. Viruses 2017, 9, 30. https://doi.org/10.3390/v9020030
Schrad JR, Young EJ, Abrahão JS, Cortines JR, Parent KN. Microscopic Characterization of the Brazilian Giant Samba Virus. Viruses. 2017; 9(2):30. https://doi.org/10.3390/v9020030
Chicago/Turabian StyleSchrad, Jason R., Eric J. Young, Jônatas S. Abrahão, Juliana R. Cortines, and Kristin N. Parent. 2017. "Microscopic Characterization of the Brazilian Giant Samba Virus" Viruses 9, no. 2: 30. https://doi.org/10.3390/v9020030