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Open AccessArticle

Characterization of a Novel Bat Adenovirus Isolated from Straw-Colored Fruit Bat (Eidolon helvum)

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Department of Virology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, 2-5-1 Shikata-cho, Kita-ku, Okayama 700-8558, Japan
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Department of Disease Control, School of Veterinary Medicine, University of Zambia, P.O. Box 32379, Lusaka 10101, Zambia
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Division of Global Epidemiology, Research Center for Zoonosis Control, Hokkaido University, N20, W10, Kita-ku, Sapporo 001-0020, Japan
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Division of Infection and Immunity, Research Center for Zoonosis Control, Hokkaido University, N20, W10, Kita-ku, Sapporo 001-0020, Japan
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Department of Paraclinical Studies, School of Veterinary Medicine, University of Zambia, P.O. Box 32379, Lusaka 10101, Zambia
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Provincial Veterinary Office, Department of Veterinary Services, Ministry of Fisheries and Livestock, P.O. Box 70416, Ndola 50100, Zambia
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Department of National Parks and Wildlife, Ministry of Tourism and Arts, Private Bag 1, Chilanga 10101, Zambia
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Hokudai Center for Zoonosis Control in Zambia, School of Veterinary Medicine, University of Zambia, P.O. Box 32379, Lusaka 10101, Zambia
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Global Institution for Collaborative Research and Education (GI-CoRE), Hokkaido University, N20, W10, Kita-ku, Sapporo 001-0020, Japan
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Division of Molecular Pathobiology, Research Center for Zoonosis Control, Hokkaido University, N20, W10, Kita-ku, Sapporo 001-0020, Japan
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Global Virus Network, 801 W Baltimore St, Baltimore, MD 21201, USA
*
Authors to whom correspondence should be addressed.
Present Address: Department of Virology 3, National Institute of Infectious Diseases, 4-7-1 Gakuen, Musashimurayama 208-0011, Japan.
Present Address: Center for Advanced Research and Education (CARE), Asahikawa Medical University, 2-1-1-1 Midorigaoka-Higashi, Asahikawa 078-8510, Japan.
Viruses 2017, 9(12), 371; https://doi.org/10.3390/v9120371
Received: 10 November 2017 / Revised: 27 November 2017 / Accepted: 30 November 2017 / Published: 4 December 2017
(This article belongs to the Section Animal Viruses)
Bats are important reservoirs for emerging zoonotic viruses. For extensive surveys of potential pathogens in straw-colored fruit bats (Eidolon helvum) in Zambia, a total of 107 spleen samples of E. helvum in 2006 were inoculated onto Vero E6 cells. The cell culture inoculated with one of the samples (ZFB06-106) exhibited remarkable cytopathic changes. Based on the ultrastructural property in negative staining and cross-reactivity in immunofluorescence assays, the virus was suspected to be an adenovirus, and tentatively named E. helvum adenovirus 06-106 (EhAdV 06-106). Analysis of the full-length genome of 30,134 bp, determined by next-generation sequencing, showed the presence of 28 open reading frames. Phylogenetic analyses confirmed that EhAdV 06-106 represented a novel bat adenovirus species in the genus Mastadenovirus. The virus shared similar characteristics of low G + C contents with recently isolated members of species Bat mastadenoviruses E, F and G, from which EhAdV 06-106 diverged by more than 15% based on the distance matrix analysis of DNA polymerase amino acid sequences. According to the taxonomic criteria, we propose the tentative new species name “Bat mastadenovirus H”. Because EhAdV 06-106 exhibited a wide in vitro cell tropism, the virus might have a potential risk as an emerging virus through cross-species transmission. View Full-Text
Keywords: adenovirus; bat; Eidolon helvum; Zambia adenovirus; bat; Eidolon helvum; Zambia
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Ogawa, H.; Kajihara, M.; Nao, N.; Shigeno, A.; Fujikura, D.; Hang’ombe, B.M.; Mweene, A.S.; Mutemwa, A.; Squarre, D.; Yamada, M.; Higashi, H.; Sawa, H.; Takada, A. Characterization of a Novel Bat Adenovirus Isolated from Straw-Colored Fruit Bat (Eidolon helvum). Viruses 2017, 9, 371.

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