Next Article in Journal
Rodent Papillomaviruses
Next Article in Special Issue
Recombinant Goose Circoviruses Circulating in Domesticated and Wild Geese in Poland
Previous Article in Journal
cfa-miR-143 Promotes Apoptosis via the p53 Pathway in Canine Influenza Virus H3N2-Infected Cells
Previous Article in Special Issue
Pressure for Pattern-Specific Intertypic Recombination between Sabin Polioviruses: Evolutionary Implications
Article Menu
Issue 12 (December) cover image

Export Article

Open AccessArticle
Viruses 2017, 9(12), 361;

Distribution and Inferred Evolutionary Characteristics of a Chimeric ssDNA Virus Associated with Intertidal Marine Isopods

Department of Microbiology, Cornell University, Ithaca, NY 14850, USA
New Visions Life Sciences, Boards of Cooperative Educational Services of New York State, Ithaca, NY 14850, USA
University of North Carolina at Wilmington, Wilmington, NC 28403, USA
Department of Natural Resources and the Cornell Biological Field Station, Cornell University, Bridgeport, NY 14850, USA
Author to whom correspondence should be addressed.
Received: 26 October 2017 / Revised: 22 November 2017 / Accepted: 23 November 2017 / Published: 26 November 2017
(This article belongs to the Special Issue Viral Recombination: Ecology, Evolution and Pathogenesis)
Full-Text   |   PDF [1665 KB, uploaded 27 November 2017]   |  


Aquatic invertebrates are common reservoirs of a rapidly expanding group of circular Rep-encoding ssDNA (CRESS-DNA) viruses. This study identified and explored the phylogenetic relationship between novel CRESS-DNA viral genotypes associated with Pacific intertidal isopods Idotea wosnesenskii, Idotea resecata, and Gnorimosphaeroma oregonensis. One genotype associated with I. wosnesenskii, IWaV278, shared sequence similarity and genomic features with Tombusviridae (ssRNA) and Circoviridae (ssDNA) genomes and was putatively assigned to the Cruciviridae clade comprising chimeric viruses. The complete genome of IWaV278 (3478 nt) was computationally completed, validated via Sanger sequencing, and exhibited sequence conservation and codon usage patterns analogous to other members of the Cruciviridae. Viral surveillance (qPCR) indicated that this virus was temporally transient (present in 2015, but not 2017), specific to I. wosnesenskii at a single collection site (Washington, DC, USA), more prevalent among male specimens, and frequently detected within exoskeletal structures. 18S rRNA sequences identified two alveolate protists associated with IWaV278-positive tissues and mechanical epibiont removal of ciliated exoskeletal structures eliminated viral detection, suggesting that the putative host of IWaV278 may be an epibiont of I. wosnesenskii. This investigation provides additional phylogenetic evidence to resolve Cruciviridae evolution and offers insight into the biogeography, specificity, and potential host of a crucivirus genotype. View Full-Text
Keywords: ssDNA virus; viromics; isopod; chimeric; intertidal; Cruciviridae; CRESS-DNA ssDNA virus; viromics; isopod; chimeric; intertidal; Cruciviridae; CRESS-DNA

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Supplementary material


Share & Cite This Article

MDPI and ACS Style

Bistolas, K.S.I.; Besemer, R.M.; Rudstam, L.G.; Hewson, I. Distribution and Inferred Evolutionary Characteristics of a Chimeric ssDNA Virus Associated with Intertidal Marine Isopods. Viruses 2017, 9, 361.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Viruses EISSN 1999-4915 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top