Maporal Hantavirus Causes Mild Pathology in Deer Mice (Peromyscus maniculatus)
Abstract
:1. Introduction
2. Materials and Methods
2.1. Ethics Statement
2.2. Experimental Infections
2.3. Tissue Processing
2.4. ELISA
2.5. Serum Neutralization Assay
2.6. Detection of Viral RNA in Tissues
2.7. Cytokine PCR
2.8. Generation of Deer Mouse Pulmonary Microvascular Endothelial Cells
2.9. PMVEC Infections
3. Results
3.1. Maporal Virus Persistently Infects Deer Mice
3.2. Histopathology and Immunohistochemistry
3.3. Gene Expression Profiling
3.4. Maporal Virus Infects Deer Mouse Pulmonary Cells
3.5. Maporal Virus RNA Accumulates in the Cellular Fraction of Deer Mouse PMVEC
4. Discussion
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Mouse # | DPI | Lungs | Heart | ||||
---|---|---|---|---|---|---|---|
Cong./Ede | MIAH | IP | PBLH | CD/apop | LIM | ||
DM 1 | 2 | + | + | - | - | - | + |
DM 2 | 2 | ++ | + | +/− | - | +/− | +/− |
DM 3 | 4 | ++ | + | +/− | + | +/− | +/− |
DM 4 | 4 | + | - | - | - | +/− | +/− |
DM 5 | 7 | ++ | - | + | + | +/− | + |
DM 6 | 7 | ++ | +/− | + | ++ | + | ++ |
DM 7 | 14 | +/− | +/− | - | +/− | - | +/− |
DM 8 | 14 | +/− | ++ | +/− | - | - | +/− |
DM 9 | 56 | + | +/− | +/− | +/− | - | +/− |
DM 10 | 56 | + | + | - | - | - | - |
Feature | MAPV | SNV | ANDV |
---|---|---|---|
Clinical Disease | None | None | None |
Outcome | Persistence | Persistence | Clearance |
Nucleocapsid Ab day 14 | Some | Some | All |
Neutralizing Ab titer | High | High | Low |
Cytokine gene expression | Low | Low | High |
Histopathology | Minimal-Mild | Minimal | None |
Nucleocapsid IHC | None | Low | None |
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McGuire, A.; Miedema, K.; Fauver, J.R.; Rico, A.; Aboellail, T.; Quackenbush, S.L.; Hawkinson, A.; Schountz, T. Maporal Hantavirus Causes Mild Pathology in Deer Mice (Peromyscus maniculatus). Viruses 2016, 8, 286. https://doi.org/10.3390/v8100286
McGuire A, Miedema K, Fauver JR, Rico A, Aboellail T, Quackenbush SL, Hawkinson A, Schountz T. Maporal Hantavirus Causes Mild Pathology in Deer Mice (Peromyscus maniculatus). Viruses. 2016; 8(10):286. https://doi.org/10.3390/v8100286
Chicago/Turabian StyleMcGuire, Amanda, Kaitlyn Miedema, Joseph R. Fauver, Amber Rico, Tawfik Aboellail, Sandra L. Quackenbush, Ann Hawkinson, and Tony Schountz. 2016. "Maporal Hantavirus Causes Mild Pathology in Deer Mice (Peromyscus maniculatus)" Viruses 8, no. 10: 286. https://doi.org/10.3390/v8100286