Next Article in Journal
Roles of HTLV-1 basic Zip Factor (HBZ) in Viral Chronicity and Leukemic Transformation. Potential New Therapeutic Approaches to Prevent and Treat HTLV-1-Related Diseases
Next Article in Special Issue
What Can We Learn from a Metagenomic Analysis of a Georgian Bacteriophage Cocktail?
Previous Article in Journal
Two Cytoplasmic Acylation Sites and an Adjacent Hydrophobic Residue, but No Other Conserved Amino Acids in the Cytoplasmic Tail of HA from Influenza A Virus Are Crucial for Virus Replication
Previous Article in Special Issue
Structure of the Receptor-Binding Carboxy-Terminal Domain of the Bacteriophage T5 L-Shaped Tail Fibre with and without Its Intra-Molecular Chaperone
Open AccessArticle

Bacteriophage Mediates Efficient Gene Transfer in Combination with Conventional Transfection Reagents

Phage Therapy Group, Division of Brain Sciences, Department of Medicine, Imperial College London, Hammersmith Hospital Campus, London W12 0NN, UK
National Nanotechnology Center, National Science and Technology Development Agency, 111 Thailand Science Park, Pathum Thani 12120, Thailand
Laboratory of Genetics, University of Balearic islands, Valldemossa Road Km. 7,5, 07122 Palma, Spain
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editor: Abram Aertsen
Viruses 2015, 7(12), 6476-6489;
Received: 19 August 2015 / Revised: 26 November 2015 / Accepted: 1 December 2015 / Published: 8 December 2015
The development of commercially available transfection reagents for gene transfer applications has revolutionized the field of molecular biology and scientific research. However, the challenge remains in ensuring that they are efficient, safe, reproducible and cost effective. Bacteriophage (phage)-based viral vectors have the potential to be utilized for general gene transfer applications within research and industry. Yet, they require adaptations in order to enable them to efficiently enter cells and overcome mammalian cellular barriers, as they infect bacteria only; furthermore, limited progress has been made at increasing their efficiency. The production of a novel hybrid nanocomplex system consisting of two different nanomaterial systems, phage vectors and conventional transfection reagents, could overcome these limitations. Here we demonstrate that the combination of cationic lipids, cationic polymers or calcium phosphate with M13 bacteriophage-derived vectors, engineered to carry a mammalian transgene cassette, resulted in increased cellular attachment, entry and improved transgene expression in human cells. Moreover, addition of a targeting ligand into the nanocomplex system, through genetic engineering of the phage capsid further increased gene expression and was effective in a stable cell line generation application. Overall, this new hybrid nanocomplex system (i) provides enhanced phage-mediated gene transfer; (ii) is applicable for laboratory transfection processes and (iii) shows promise within industry for large-scale gene transfer applications. View Full-Text
Keywords: Bacteriophage; phage-based gene delivery; phage biotechnology; transfection; calcium phosphate Bacteriophage; phage-based gene delivery; phage biotechnology; transfection; calcium phosphate
Show Figures

Figure 1

MDPI and ACS Style

Donnelly, A.; Yata, T.; Bentayebi, K.; Suwan, K.; Hajitou, A. Bacteriophage Mediates Efficient Gene Transfer in Combination with Conventional Transfection Reagents. Viruses 2015, 7, 6476-6489.

Show more citation formats Show less citations formats

Article Access Map by Country/Region

Search more from Scilit
Back to TopTop