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Open AccessArticle

Psoralen Inactivation of Viruses: A Process for the Safe Manipulation of Viral Antigen and Nucleic Acid

1
National Biodefense Analysis and Countermeasures Center (NBACC), Fort Detrick, MD 21702, USA
2
Naval Medical Research Center, Silver Spring, MD 20910, USA
3
University of Maryland, College Park, MD 20742, USA
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editor: Curt Hagedorn
Viruses 2015, 7(11), 5875-5888; https://doi.org/10.3390/v7112912
Received: 22 September 2015 / Revised: 3 November 2015 / Accepted: 4 November 2015 / Published: 12 November 2015
(This article belongs to the Section Antivirals & Vaccines)
High consequence human pathogenic viruses must be handled at biosafety level 2, 3 or 4 and must be rendered non-infectious before they can be utilized for molecular or immunological applications at lower biosafety levels. Here we evaluate psoralen-inactivated Arena-, Bunya-, Corona-, Filo-, Flavi- and Orthomyxoviruses for their suitability as antigen in immunological processes and as template for reverse transcription PCR and sequencing. The method of virus inactivation using a psoralen molecule appears to have broad applicability to RNA viruses and to leave both the particle and RNA of the treated virus intact, while rendering the virus non-infectious. View Full-Text
Keywords: virus; inactivation; biocontainment; BSL-4; BSL-3; psoralen; Trizol LS; IFA; safety; biosafety; PCR; reverse transcription; sequencing; antibody; sterility virus; inactivation; biocontainment; BSL-4; BSL-3; psoralen; Trizol LS; IFA; safety; biosafety; PCR; reverse transcription; sequencing; antibody; sterility
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Schneider, K.; Wronka-Edwards, L.; Leggett-Embrey, M.; Walker, E.; Sun, P.; Ondov, B.; Wyman, T.H.; Rosovitz, M.; Bohn, S.S.; Burans, J.; Kochel, T. Psoralen Inactivation of Viruses: A Process for the Safe Manipulation of Viral Antigen and Nucleic Acid. Viruses 2015, 7, 5875-5888.

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