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The Standard Scrapie Cell Assay: Development, Utility and Prospects

Centre for Prions and Protein Folding Diseases, University of Alberta, Alberta, T6G2M8, Canada
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Academic Editor: Byron Caughey
Viruses 2015, 7(1), 180-198; https://doi.org/10.3390/v7010180
Received: 5 November 2014 / Accepted: 6 January 2015 / Published: 16 January 2015
(This article belongs to the Special Issue Recent Developments in the Prion Field)
Prion diseases are a family of fatal neurodegenerative diseases that involve the misfolding of a host protein, PrPC. Measuring prion infectivity is necessary for determining efficacy of a treatment or infectivity of a prion purification procedure; animal bioassays are, however, very expensive and time consuming. The Standard Scrapie Cell Assay (SSCA) provides an alternative approach. The SSCA facilitates quantitative in vitro analysis of prion strains, titres and biological properties. Given its robust nature and potential for high throughput, the SSCA has substantial utility for in vitro characterization of prions and can be deployed in a number of settings. Here we provide an overview on establishing the SSCA, its use in studies of disease dissemination and pathogenesis, potential pitfalls and a number of remaining challenges. View Full-Text
Keywords: standard scrapie cell assay; prion standard scrapie cell assay; prion
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Van der Merwe, J.; Aiken, J.; Westaway, D.; McKenzie, D. The Standard Scrapie Cell Assay: Development, Utility and Prospects. Viruses 2015, 7, 180-198.

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