Pseudo-Mannosylated DC-SIGN Ligands as Potential Adjuvants for HIV Vaccines
Abstract
:1. Introduction
2. Results and Discussion
2.1.Inhibition of HIV-1 Infection of Human Cervical Tissue by Polyman 19
2.2. Polyman 19 Toxicity towards Cervical Explants
2.3. Modulation of Innate Immune Responses by Polyman 19
3. Experimental
3.1. Human Cervical Explants Infection
3.2. Tetrazolium Salt 1-(4,5-Dimethylthiazol-2-yl)-3,5-diphenyl Formazan (MTT) Assay
3.3. Human Monocyte Isolation
3.4. Differentiation of DCs from Monocytes
3.5. Flow Cytometry
3.6. RNA Extraction and Retrotranscription
3.7. Real Time PCR
4. Conclusions
Acknowledgments
Authors’ Contribution
Conflicts of Interest
References and Notes
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Berzi, A.; Varga, N.; Sattin, S.; Antonazzo, P.; Biasin, M.; Cetin, I.; Trabattoni, D.; Bernardi, A.; Clerici, M. Pseudo-Mannosylated DC-SIGN Ligands as Potential Adjuvants for HIV Vaccines. Viruses 2014, 6, 391-403. https://doi.org/10.3390/v6020391
Berzi A, Varga N, Sattin S, Antonazzo P, Biasin M, Cetin I, Trabattoni D, Bernardi A, Clerici M. Pseudo-Mannosylated DC-SIGN Ligands as Potential Adjuvants for HIV Vaccines. Viruses. 2014; 6(2):391-403. https://doi.org/10.3390/v6020391
Chicago/Turabian StyleBerzi, Angela, Norbert Varga, Sara Sattin, Patrizio Antonazzo, Mara Biasin, Irene Cetin, Daria Trabattoni, Anna Bernardi, and Mario Clerici. 2014. "Pseudo-Mannosylated DC-SIGN Ligands as Potential Adjuvants for HIV Vaccines" Viruses 6, no. 2: 391-403. https://doi.org/10.3390/v6020391