Next Article in Journal
From Crescent to Mature Virion: Vaccinia Virus Assembly and Maturation
Previous Article in Journal
Molecular Modeling of Prion Transmission to Humans
Open AccessArticle

Effect of Bacteriophage Infection in Combination with Tobramycin on the Emergence of Resistance in Escherichia coli and Pseudomonas aeruginosa Biofilms

1
Clinical Laboratory Science Program, Texas State University, 601 University Drive, San Marcos, TX 78666, USA
2
Department of Biology, Texas State University, San Marcos, 601 University Drive, TX 78666, USA
*
Author to whom correspondence should be addressed.
Viruses 2014, 6(10), 3778-3786; https://doi.org/10.3390/v6103778
Received: 20 August 2014 / Revised: 26 September 2014 / Accepted: 26 September 2014 / Published: 3 October 2014
(This article belongs to the Section Bacterial Viruses)
Bacteriophage infection and antibiotics used individually to reduce biofilm mass often result in the emergence of significant levels of phage and antibiotic resistant cells. In contrast, combination therapy in Escherichia coli biofilms employing T4 phage and tobramycin resulted in greater than 99% and 39% reduction in antibiotic and phage resistant cells, respectively. In P. aeruginosa biofilms, combination therapy resulted in a 60% and 99% reduction in antibiotic and PB-1 phage resistant cells, respectively. Although the combined treatment resulted in greater reduction of E. coli CFUs compared to the use of antibiotic alone, infection of P. aeruginosa biofilms with PB-1 in the presence of tobramycin was only as effective in the reduction of CFUs as the use of antibiotic alone. The study demonstrated phage infection in combination with tobramycin can significantly reduce the emergence of antibiotic and phage resistant cells in both E. coli and P. aeruginosa biofilms, however, a reduction in biomass was dependent on the phage-host system. View Full-Text
Keywords: biofilms; bacteriophage; antibiotic; resistance; mixed therapy biofilms; bacteriophage; antibiotic; resistance; mixed therapy
Show Figures

Figure 1

MDPI and ACS Style

Coulter, L.B.; McLean, R.J.C.; Rohde, R.E.; Aron, G.M. Effect of Bacteriophage Infection in Combination with Tobramycin on the Emergence of Resistance in Escherichia coli and Pseudomonas aeruginosa Biofilms. Viruses 2014, 6, 3778-3786.

Show more citation formats Show less citations formats

Article Access Map by Country/Region

1
Only visits after 24 November 2015 are recorded.
Search more from Scilit
 
Search
Back to TopTop