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Correction to Viruses 2024, 16(3), 470.
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Correction

Correction: Villalba et al. Development and Validation of Three Triplex Real-Time RT-PCR Assays for Typing African Horse Sickness Virus: Utility for Disease Control and Other Laboratory Applications. Viruses 2024, 16, 470

by
Rubén Villalba
1,
Cristina Tena-Tomás
2,
María José Ruano
1,
Marta Valero-Lorenzo
1,
Ana López-Herranz
1,
Cristina Cano-Gómez
1 and
Montserrat Agüero
1,*
1
Laboratorio Central de Veterinaria, Ministry of Agriculture, Fisheries and Food, 28110 Algete, Spain
2
Tecnologías y Servicios Agrarios, S.A. (TRAGSATEC), 28037 Madrid, Spain
*
Author to whom correspondence should be addressed.
Viruses 2026, 18(9), 963; https://doi.org/10.3390/v18090963
Submission received: 29 July 2026 / Accepted: 13 August 2026 / Published: 2 September 2026
(This article belongs to the Section Animal Viruses)
Versions Notes

Error in Table 1

In the original publication [1], there was a mistake in Table 1. Serotype-specific rRT-PCR primers and probes targeting VP2 AHSV genes for serotypes 1–9. F: Forward, R: Reverse, P: Probe. as published. The mistake lies in the nucleotide sequences of some primers and/or probes, specifically Serotype 2 (AHS-2F), Serotype 3 (AHS-3F and AHS-3R), Serotype 7 (AHS-7F, AHS-7R, and AHS-7P), and Serotype 9 (AHS-9R and AHS-9P).
Table 1. Serotype-specific rRT-PCR primers and probes targeting VP2 AHSV genes for serotypes 1–9. F: Forward, R: Reverse, P: Probe.
Table 1. Serotype-specific rRT-PCR primers and probes targeting VP2 AHSV genes for serotypes 1–9. F: Forward, R: Reverse, P: Probe.
AHSVPrimers (F/R) and ProbeSequence 5′-3′
Serotype 1AHS-1FGCAAGCGCTGGCACTTG
AHS-1RTTCGAACTCATTCCTTACATCAACA
AHS1PFAM-AATGTCTTAGATCGTCAACT-MGB
Serotype 2AHS-2FCGGAAACTYTGTATTGCCAAA
AHS-2RTTGTCRTCCTGATCAACCCTAA
AHS-2PCy5-TGAAGGTGCTTACCCGATCTTTCCACA-BBQ
Serotype 3AHS-3FAATTATTACAGCGGAGAATGCAGTT
AHS-3RGGTTATGAGTGGGGTGCGA
AHS-3PFAM-AGAGTTGAGGTTGCGGGA-MGB
Serotype 4AHS-4FTGAGGTGGAACACGAYATGTC
AHS-4RGATATGCCCCCTCACAYCTGA
AHS-4PVIC-TATCGGRATTTATGTACAATGAG-MGB
Serotype 5AHS-5FGAAGAGACAGGCGATTCAAATGA
AHS-5RAAAGCCACCCTTTTTGGTACAAA
AHS-5PNED-TGTTGARATGCTGAGGC-MGB
Serotype 6AHS-6FAGCCAGGGCTTCTTTGCA
AHS-6RCTCATGTTCAACCCACTGTACATTAA
AHS-6PVIC-GTCATCACCGTAAGCG-MGB
Serotype 7AHS-7FAGCCAGGGCTTCTTTGCA
AHS-7RCTCATGTTCAACCCACTGTACATTAA
AHS-7PVIC-GTCATCACCGTAAGCG-MGB
Serotype 8AHS-8FGAAATTATCAGCGGACTGACTAAGAA
AHS-8RAAACATCTACCTTTTGCGAATCTTG
AHS-8PNED-ACGTGATTCTTTTCCC-MGB
Serotype 9AHS-9FTACTGTGTCGGTGAGGGATTTT
AHS-9RGCCACGACCGGATATGA
AHS-9PFAM-AAACAAACGAAATGTGAA-MGB
The corrected Table 1. Serotype-specific rRT-PCR primers and probes targeting VP2 AHSV genes for serotypes 1–9. F: Forward, R: Reverse, P: Probe. appears below. The authors state that the scientific conclusions are unaffected. This correction was approved by the Academic Editor. The original publication has also been updated.
Table 1. Serotype-specific rRT-PCR primers and probes targeting VP2 AHSV genes for serotypes 1–9. F: Forward, R: Reverse, P: Probe.
Table 1. Serotype-specific rRT-PCR primers and probes targeting VP2 AHSV genes for serotypes 1–9. F: Forward, R: Reverse, P: Probe.
AHSV Primers (F/R) and ProbeSequence 5′-3′
Serotype 1AHS-1FGCAAGCGCTGGCACTTG
AHS-1RTTCGAACTCATTCCTTACATCAACA
AHS1PFAM-AATGTCTTAGATCGTCAACT-MGB
Serotype 2AHS-2FCGGAAACYATGTATTGCCAA
AHS-2RTTGTCRTCCTGATCAACCCTAA
AHS-2PCy5-TGAAGGTGCTTACCCGATCTTTCCACA-BBQ
Serotype 3AHS-3FAATTATTACAGCGGARAATGCAGTT
AHS-3RTCGCACCCCACTCATAACC
AHS-3PFAM-AGAGTTGAGGTTGCGGGA-MGB
Serotype 4AHS-4FTGAGGTGGAACACGAYATGTC
AHS-4RGATATGCCCCCTCACAYCTGA
AHS-4PVIC-TATCGGRATTTATGTACAATGAG-MGB
Serotype 5AHS-5FGAAGAGACAGGCGATTCAAATGA
AHS-5RAAAGCCACCCTTTTTGGTACAAA
AHS-5PNED-TGTTGARATGCTGAGGC-MGB
Serotype 6AHS-6FAGCCAGGGCTTCTTTGCA
AHS-6RCTCATGTTCAACCCACTGTACATTAA
AHS-6PVIC-GTCATCACCGTAAGCG-MGB
Serotype 7AHS-7FGAGTGGGGCGCAACAAATC
AHS-7RCCGGATATGCACCCTCACAT
AHS-7PVIC-CGATATCTGAGTTCATGTATGAA-MGB
Serotype 8AHS-8FGAAATTATCAGCGGACTGACTAAGAA
AHS-8RAAACATCTACCTTTTGCGAATCTTG
AHS-8PNED-ACGTGATTCTTTTCCC-MGB
Serotype 9AHS-9FTACTGTGTCGGTGAGGGATTTT
AHS-9RGGCACGACCGGATATGA
AHS-9PFAM-TTCACATTTCGTTTGTTT-MGB

Reference

  1. Villalba, R.; Tena-Tomás, C.; Ruano, M.J.; Valero-Lorenzo, M.; López-Herranz, A.; Cano-Gómez, C.; Agüero, M. Development and Validation of Three Triplex Real-Time RT-PCR Assays for Typing African Horse Sickness Virus: Utility for Disease Control and Other Laboratory Applications. Viruses 2024, 16, 470. [Google Scholar] [CrossRef] [Scilit] [PubMed]
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MDPI and ACS Style

Villalba, R.; Tena-Tomás, C.; Ruano, M.J.; Valero-Lorenzo, M.; López-Herranz, A.; Cano-Gómez, C.; Agüero, M. Correction: Villalba et al. Development and Validation of Three Triplex Real-Time RT-PCR Assays for Typing African Horse Sickness Virus: Utility for Disease Control and Other Laboratory Applications. Viruses 2024, 16, 470. Viruses 2026, 18, 963. https://doi.org/10.3390/v18090963

AMA Style

Villalba R, Tena-Tomás C, Ruano MJ, Valero-Lorenzo M, López-Herranz A, Cano-Gómez C, Agüero M. Correction: Villalba et al. Development and Validation of Three Triplex Real-Time RT-PCR Assays for Typing African Horse Sickness Virus: Utility for Disease Control and Other Laboratory Applications. Viruses 2024, 16, 470. Viruses. 2026; 18(9):963. https://doi.org/10.3390/v18090963

Chicago/Turabian Style

Villalba, Rubén, Cristina Tena-Tomás, María José Ruano, Marta Valero-Lorenzo, Ana López-Herranz, Cristina Cano-Gómez, and Montserrat Agüero. 2026. "Correction: Villalba et al. Development and Validation of Three Triplex Real-Time RT-PCR Assays for Typing African Horse Sickness Virus: Utility for Disease Control and Other Laboratory Applications. Viruses 2024, 16, 470" Viruses 18, no. 9: 963. https://doi.org/10.3390/v18090963

APA Style

Villalba, R., Tena-Tomás, C., Ruano, M. J., Valero-Lorenzo, M., López-Herranz, A., Cano-Gómez, C., & Agüero, M. (2026). Correction: Villalba et al. Development and Validation of Three Triplex Real-Time RT-PCR Assays for Typing African Horse Sickness Virus: Utility for Disease Control and Other Laboratory Applications. Viruses 2024, 16, 470. Viruses, 18(9), 963. https://doi.org/10.3390/v18090963

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