A New Human SCARB2 Knock-In Mouse Model for Studying Coxsackievirus A16 and Its Neurotoxicity
Abstract
:1. Introduction
2. Materials and Methods
2.1. Ethics Statement
2.2. Cell and Virus
2.3. Virus Enrichment and Titration
2.4. Generation of ROSA26-Targeted hSCARB2 KI F0 Mice via Cytoplasmic Microinjection
2.5. Genotyping
2.6. Western Blotting
2.7. CVA16 Infection
2.8. Real-Time PCR
2.9. Histopathological and Immunohistochemical Staining
2.10. Immunofluorescence Staining
2.11. Statistical Analysis
3. Results
3.1. Generation of ROSA26-hSCARB2 KI Mice
3.2. The Expression of hSCARB2 in KI Mice
3.3. Susceptibility of hSCARB2 Mice to CVA16 Infection
3.4. Dynamic Changes in the CVA16 Viral Load and Detection of Viral Expression in Infected KI Mice
3.5. Histopathological Examination of CVA16-Infected Mice
3.6. Activation of Glial Cells in CVA16-Infected Brains
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Wu, H.; Wang, Z.; Zhang, Y.; Hu, L.; Yang, J.; Zhang, C.; Lou, M.; Pi, N.; Wang, Q.; Fan, S.; et al. A New Human SCARB2 Knock-In Mouse Model for Studying Coxsackievirus A16 and Its Neurotoxicity. Viruses 2025, 17, 423. https://doi.org/10.3390/v17030423
Wu H, Wang Z, Zhang Y, Hu L, Yang J, Zhang C, Lou M, Pi N, Wang Q, Fan S, et al. A New Human SCARB2 Knock-In Mouse Model for Studying Coxsackievirus A16 and Its Neurotoxicity. Viruses. 2025; 17(3):423. https://doi.org/10.3390/v17030423
Chicago/Turabian StyleWu, Haiting, Ziou Wang, Yiwei Zhang, Lingfeng Hu, Jinling Yang, Caixing Zhang, Mumeng Lou, Na Pi, Qiyan Wang, Shengtao Fan, and et al. 2025. "A New Human SCARB2 Knock-In Mouse Model for Studying Coxsackievirus A16 and Its Neurotoxicity" Viruses 17, no. 3: 423. https://doi.org/10.3390/v17030423
APA StyleWu, H., Wang, Z., Zhang, Y., Hu, L., Yang, J., Zhang, C., Lou, M., Pi, N., Wang, Q., Fan, S., & Huang, Z. (2025). A New Human SCARB2 Knock-In Mouse Model for Studying Coxsackievirus A16 and Its Neurotoxicity. Viruses, 17(3), 423. https://doi.org/10.3390/v17030423