mRNA Splicing of UL44 and Secretion of Alphaherpesvirinae Glycoprotein C (gC) Is Conserved among the Mardiviruses
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cell Cultures
2.2. Viruses
2.3. Western Blot Analysis of Secreted gC Proteins
2.4. RNA Extraction and RT-PCR Analysis
2.5. DNA Sequencing
2.6. Generation of Recombinant (r)HVT
2.7. Generation of Recombinant 301B/1 with Mutations in the Donor, BP, and Acceptor Sites
3. Results
3.1. Generation of rHVT
3.1.1. Generation of rHVT47G
3.1.2. Generation of rHVT Lacking gC or Expressing HA-Tagged HVT gC or MDV gC
3.2. Secretion of HVT gC Protein
3.3. Identification of HVT and 301B/1 UL44 (gC) mRNA Splice Variants
3.4. Conserved mRNA Splicing Donor and Acceptor Sequences
3.5. Modification of 301B/1 Donor, BP, and Acceptor Sequences Does Not Rescue gC145 Splicing
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Virus 1 | Gene 2 | Direction | Sequence (5′→3′) 3 | Purpose 4 |
---|---|---|---|---|
HVT | UL47 | Forward | CGTGGCAAGTCTGCGGTTGG | Sequencing |
Reverse | CAACCACATTGCTTCCGC | |||
HVT | UL44 | Forward | CGTAAGCTTTGTGTTTTATTGAGCGGTCG | Cloning |
Reverse | CGTTCTAGATTTGGCCGCTGCGTGATACC | |||
HVT | UL44 | Forward | CGACGGGATCCCCAGGGTTCTTTCTGGACTAGTCCTACACCCCGTGGAAATAGGGATAACAGGGTAATCGATTT | Shuttle Vector |
Reverse | TTAACGGATCCGCCAGTGTTACAACCAATTAACC | |||
MDV | UL44 | Forward | TATCTTCCCTCATGCTCACG | RT-PCR (full) |
Forward | CTCGGAAAGATCGCGATGGA | RT-PCR (diff) | ||
Reverse | CACAACTACATAACAATGAGATTATAATCG | RT-PCR (full/diff) | ||
301B | UL44 | Forward | CCCATGCACGCGTCACG | RT-PCR (full) |
Forward | GGGAGCCACCCTGGTATCTA | RT-PCR (diff) | ||
Reverse | GACGGACGAAAGTAATATGTATTTTTTCCCG | RT-PCR (full/diff) | ||
HVT | UL44 | Forward | CCGCAAGTATATGGTTTCCAACATGC | RT-PCR (full) |
Forward | GTGTGCCAACGACCCGCATC | RT-PCR (diff) | ||
Reverse | CTTAATTCCGCCCCGGTAGG | RT-PCR (full/diff) |
Modification 1 | Direction | Sequence (5′→3′) 2 |
---|---|---|
eGFP at C-term of HVT UL47 | Forward | gatgccaaagatgtaatatttgaaccgcgcccttttaaaATGgtgagcaagggcgaggag |
Reverse | aacacccctccgcgtcgggcaagagtttcggaagcacgCTActtgtacagctcgtccatgccg | |
Removal of HVT gC (ΔgC) | Forward | tttataccatattacgcatctatcgaaacttgttcgagaaccgcaagtatTAAgattaaccatcgtattagggataacagggtaatcgattt |
Reverse | ggttataacacttaataatttttatatcacatacgatggttaatcTTAatacttgcggttctcgaacagccagtgttacaaccaattaacc | |
HVT gC into rHΔgC | Forward | tttataccatattacgcatctatcgaaacttgtgttttattgagcggtcg |
Reverse | ggttataacacttaataatttttatatcacctgatcagcgggtttaaacg | |
HA at N-term of HVT gC | Forward | agttctgtctttacagcaaacctcttgtgccggattgccctatccgtatgatgtgccggattatgcgtagggataacagggtaatcgattt |
Reverse | ttgaaagttaggatatgatgggtatcgacgttatgcgcataatccggcacatcatacggatagggcaatccggcagccagtgttacaaccaattaacc | |
MDV gC into rHΔgC | Forward | tttataccatattacgcatctatcgaaacttgttcgagaatatcttccctcatgctcacg |
Reverse | ggttataacacttaataatttttatatcacatacgatggtcataacaatgagattataat |
Modification 1 | Direction | Sequence (5′→3′) 2 |
---|---|---|
UL44 C1002G (gC145 donor) | Forward | agcagggaaatatgatgagtactacgaacgccactgcaatcccgacGgtatatcatcatccccggtagggataacagggtaatcgattt |
Reverse | ttgcatacccatctttgaaagccagggatatccggggatgatgatataCcgtcgggattgcagtgggccagtgttacaaccaattaacc | |
UL44 T1122C (BP) | Forward | ggaattaccatacgatggttagtacacgatgaacccaaacctaaCacaacttatgatacttagggataacagggtaatcgattt |
Reverse | cttgagggtcctgcaaagacctgtaaccacagtatcataagttgtGttaggtttgggttcgccagtgttacaaccaattaacc | |
UL44 MDV acceptor (Macc) | Forward | tagtacacgatgaacccaaacctaatacaacttatAatactgtggttacaggtctCtgcCggaccAtcGaTtagggataacagggtaatcgattt |
Forward * | tagtacacgatgaacccaaacctaaCacaacttatAatactgtggttacaggtctCtgcCggaccAtcGaTtagggataacagggtaatcgattt | |
Reverse | aatattcggctgatgatatttctatgccgAtCgaTggtccGgcaGagacctgtaaccacagtatTgccagtgttacaaccaattaacc |
Virus | Name 1 | Start 2 | End 3 | Score 4 | Exon/Intron (5′-3′) |
---|---|---|---|---|---|
MDV | MD1 | 162 | 176 | 0.90 | aactgag/gtacctca |
MD2 | 187 | 201 | 0.29 | acagaaa/gtgtgtca | |
MD3 | 611 | 625 | 0.25 | gaaacaa/gtacttca | |
MD4 | 751 | 765 | 0.18 | tacatac/gtgtgtgt | |
MD5 (gC145) | 1074 | 1088 | 0.24 | cccgacg/gtatatca | |
MD6 (gC104) | 1115 | 1129 | 0.69 | aagatgg/gtatgcaa | |
MD7 | 1166 | 1180 | 0.95 | tacggtg/gttagtac | |
MD8 | 1392 | 1406 | 0.16 | acccatg/gttattac | |
HVT | HD1 (gC145) | 1050 | 1064 | 0.15 | accgacg/gtatatta |
HD2 (gC104) | 1091 | 1105 | 0.69 | aagatgg/gtatgcaa | |
HD3 | 1142 | 1156 | 0.54 | tgaggtg/gttagttc | |
HD4 | 1411 | 1425 | 0.12 | tgaggtg/gttagttc | |
301B/1 | 3D1 | 533 | 547 | 0.18 | gagacaa/gtacttca |
3D2 | 823 | 837 | 0.99 | acaccac/gtaaggac | |
3D3 | 879 | 893 | 0.14 | caccctg/gtatctac | |
3D4 (gC104) | 1037 | 1051 | 0.69 | aagatgg/gtatgcaa | |
3D5 | 1088 | 1102 | 0.98 | tacgatg/gttagtac |
Virus | Name 1 | Start 2 | End 3 | Score 4 | Intron/Exon (5′-3′) |
---|---|---|---|---|---|
MDV | MA1 | 170 | 210 | 0.70 | tacctcatgcaccttccaca/gaaagtgtgtcaacaaattcg |
MA2 | 413 | 453 | 0.89 | aatggtccaactttgttcta/gatctgatctttaacccaatt | |
MA3 | 719 | 759 | 0.19 | attttaatcggcctttaata/gataaacatatttacatacgt | |
MA4 | 782 | 822 | 0.20 | tggatgtactggcccctcca/gtcctcagcggagaaaattac | |
MA5 | 822 | 862 | 0.11 | caaggcatcttgtatcgtta/gacacttttatccccctggat | |
MA6 | 1097 | 1137 | 0.23 | gtttatccctggcttttaaa/gatgggtatgcaatatgtact | |
MA7 (gC104/145) | 1205 | 1245 | 0.73 | cttataatactgtggttaca/ggtctctgccggaccatcgat | |
MA8 | 1233 | 1273 | 0.42 | ccggaccatcgatcgccata/gaaatctcctcagccgcattc | |
MA9 | 1301 | 1341 | 0.31 | aatatacgtgcagactcata/ggctaccccttcgatgaagat | |
MA10 | 1421 | 1461 | 0.48 | tgggattggctgtaatttta/gggatggggataatcatgact | |
HVT | HA1 | 58 | 98 | 0.96 | tttctagttctgtctttaca/gcaaacctcttgtgccggatt |
HA2 | 155 | 195 | 0.47 | ccgatggcgttcctttgtca/gaggtgcccaattcgcctacg | |
HA3 | 323 | 363 | 0.13 | tcgtattccttaacaataca/ggaagaattttgtgtgacctt | |
HA4 | 347 | 387 | 0.33 | gaattttgtgtgaccttata/gtcgaccccccttcagacgat | |
HA5 | 362 | 402 | 0.38 | ttatagtcgaccccccttca/gacgatgaatggtccaacttc | |
HA6 | 758 | 798 | 0.69 | tggatgtattggcccctcca/gttctcagcggagaaaactac | |
HA7 | 967 | 1007 | 0.84 | ggactcgaatctcctccaaa/ggtttcctgcttggtagcgtg | |
HA8 | 983 | 1023 | 0.28 | caaaggtttcctgcttggta/gcgtggaggcaaggcgatatg | |
HA9 (gC104/145) | 1181 | 1221 | 0.51 | cttatgatactgtggttaca/ggtctctgcaggaccatcgat | |
HA10 | 1191 | 1231 | 0.81 | tgtggttacaggtctctgca/ggaccatcgatcgttatagaa | |
HA11 | 1209 | 1249 | 0.58 | caggaccatcgatcgttata/gaaatctcgccagtcggattc | |
HA12 | 1237 | 1277 | 0.37 | gccagtcggattccagtcca/ggacaactgggcgaaaacgaa | |
HA13 | 1379 | 1419 | 0.29 | taacaattacggccgttcta/ggactggccttgtttttaggt | |
HA14 | 1397 | 1437 | 0.95 | taggactggccttgttttta/ggtattggtatcattatcaca | |
301B/1 | 3A1 | 170 | 210 | 0.17 | ttcctgcatccccgccggca/ggggagaaagaggagagccac |
3A2 | 227 | 267 | 0.13 | cgcgtaggatgcctagtata/gtttgcgataaagaagaagtt | |
3A3 | 292 | 332 | 0.56 | cgtttcgtgtgcactcttaa/gatcgcccctccctccgacaa | |
3A4 | 641 | 681 | 0.45 | ctttcaatcggcctttacta/gataagcacgtgtacatccgc | |
3A5 | 711 | 751 | 0.43 | tctagccccccccgtcctca/gtggcgataagtacaaggctt | |
3A6 | 744 | 784 | 0.26 | caaggcttcatgcatcgtta/ggcatttttatccaccgggct | |
3A7 | 913 | 953 | 0.36 | gccatcgaccctcctcccaa/gatttcatgtctggtagcctg | |
3A8 | 929 | 969 | 0.17 | ccaagatttcatgtctggta/gcctggaagcagggaaatatg | |
3A9 (gC104/145) | 1127 | 1167 | 0.56 | cttatgatactgtggttaca/ggtctttgcaggaccctcaag | |
3A10 | 1137 | 1177 | 0.85 | tgtggttacaggtctttgca/ggaccctcaagcggcatagaa | |
3A11 | 1313 | 1353 | 0.10 | cccccatggttctcgcgata/gcggctgttgtgggactagct |
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Xu, H.; Vega-Rodriguez, W.; Campos, V.; Jarosinski, K.W. mRNA Splicing of UL44 and Secretion of Alphaherpesvirinae Glycoprotein C (gC) Is Conserved among the Mardiviruses. Viruses 2024, 16, 782. https://doi.org/10.3390/v16050782
Xu H, Vega-Rodriguez W, Campos V, Jarosinski KW. mRNA Splicing of UL44 and Secretion of Alphaherpesvirinae Glycoprotein C (gC) Is Conserved among the Mardiviruses. Viruses. 2024; 16(5):782. https://doi.org/10.3390/v16050782
Chicago/Turabian StyleXu, Huai, Widaliz Vega-Rodriguez, Valeria Campos, and Keith W. Jarosinski. 2024. "mRNA Splicing of UL44 and Secretion of Alphaherpesvirinae Glycoprotein C (gC) Is Conserved among the Mardiviruses" Viruses 16, no. 5: 782. https://doi.org/10.3390/v16050782
APA StyleXu, H., Vega-Rodriguez, W., Campos, V., & Jarosinski, K. W. (2024). mRNA Splicing of UL44 and Secretion of Alphaherpesvirinae Glycoprotein C (gC) Is Conserved among the Mardiviruses. Viruses, 16(5), 782. https://doi.org/10.3390/v16050782