Exploring Viral Genome Profile in Mpox Patients during the 2022 Outbreak, in a North-Eastern Centre of Italy
Abstract
:1. Introduction
2. Materials and Methods
2.1. Clinical Samples
2.2. DNA Extraction, Library Preparation and NGS
2.3. Bioinformatics Analysis
- -
- Quality control (QC), performed using FastQC v.0.11.9;
- -
- Adapter removal, performed using Fastp v.0.23.2;
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- Contamination control, performed using Kraken2 v. 2.1.2;
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- Alignment of the reads to the MPXV reference genome (NC_063383.1), performed using BWA-mem2 v. 2.2.1;
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- Genome assembly, performed using iVar consensus v.1.3.1 tool (options “-aa -A -d o -Q 0”).
2.4. GISAID Data Download
2.5. Sanger Sequencing
2.6. Ethics
3. Results
3.1. QC Analyses, Read Alignment and Consensus Generation
3.2. Variant Calling and Annotation
3.3. APOBEC3-Driven Mutations
3.4. Phylogenetic Analysis
3.5. APOBEC3-Driven Mutations
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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(A) | |||||||||||
ID | EPID | DoC(X) | SNV | GENE/AA SUBs | GENE FUNCTION | READ FREQ | |||||
P4 | EPI_ISL_16467111 | 91 | C1092T | OPG001:D162N | Chemokine binding protein | 78% mutated 22% wild-type | |||||
P2 | EPI_ISL_14786346 | 102 | G2333A | OPG002:S140F | Crm-B-secreted TNF-alpha-receptor-like protein | 72% mutated 28% wild-type | |||||
P4 | EPI_ISL_16467111 | 107 | G37152A | OPG056:S628F | EEV maturation protein | 100% mutated | |||||
P3 | - | 23 | 100% mutated | ||||||||
P1 | EPI_ISL_14786290 | 45 | C83293T | OPG105:T723I | DNA-dependent RNA polymerase subunit rpo147 | 100% mutated | |||||
P2 | EPI_ISL_14786346 | 70 | G159023A | OPG185:R36Q | Hemagglutinin | 77% mutated 23% wild-type | |||||
P4 | EPI_ISL_16467111 | 110 | G171341T | OPG199:L118F | Serpin | 80% mutated 20% wild-type | |||||
P3 | - | 11 | 27% mutated 73% wild-type | ||||||||
P4 | EPI_ISL_16467111 | 92 | C186990T | OPG210:R1879W | B22R family protein | 80% mutated 20% wild-type | |||||
P3 | - | 13 | 54% mutated 46% wild-type | ||||||||
(B) | |||||||||||
NGS | SANGER | ||||||||||
P2 | P3 | P4 | P2 | P3 | P4 | ||||||
OPG185:G159023A | 77% mutated 23% wild-type | wild-type and mutated | - | - | |||||||
OPG199:C171341T | 27% mutated 73% wild-type | 80% mutated 20% wild-type | - | wild-type | mutated | ||||||
OPG210:C186990T | 54% mutated 46% wild-type | 80% mutated 20% wild-type | - | wild-type | mutated |
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Share and Cite
Deiana, M.; Lavezzari, D.; Mori, A.; Accordini, S.; Pomari, E.; Piubelli, C.; Malagò, S.; Cordioli, M.; Ronzoni, N.; Angheben, A.; et al. Exploring Viral Genome Profile in Mpox Patients during the 2022 Outbreak, in a North-Eastern Centre of Italy. Viruses 2024, 16, 726. https://doi.org/10.3390/v16050726
Deiana M, Lavezzari D, Mori A, Accordini S, Pomari E, Piubelli C, Malagò S, Cordioli M, Ronzoni N, Angheben A, et al. Exploring Viral Genome Profile in Mpox Patients during the 2022 Outbreak, in a North-Eastern Centre of Italy. Viruses. 2024; 16(5):726. https://doi.org/10.3390/v16050726
Chicago/Turabian StyleDeiana, Michela, Denise Lavezzari, Antonio Mori, Silvia Accordini, Elena Pomari, Chiara Piubelli, Simone Malagò, Maddalena Cordioli, Niccolò Ronzoni, Andrea Angheben, and et al. 2024. "Exploring Viral Genome Profile in Mpox Patients during the 2022 Outbreak, in a North-Eastern Centre of Italy" Viruses 16, no. 5: 726. https://doi.org/10.3390/v16050726
APA StyleDeiana, M., Lavezzari, D., Mori, A., Accordini, S., Pomari, E., Piubelli, C., Malagò, S., Cordioli, M., Ronzoni, N., Angheben, A., Tacconelli, E., Capobianchi, M. R., Gobbi, F. G., & Castilletti, C. (2024). Exploring Viral Genome Profile in Mpox Patients during the 2022 Outbreak, in a North-Eastern Centre of Italy. Viruses, 16(5), 726. https://doi.org/10.3390/v16050726