CD8+ T Cells Mediate Lethal Lung Pathology in the Absence of PD-L1 and Type I Interferon Signalling following LCMV Infection
Abstract
:1. Introduction
2. Materials and Methods
2.1. Mice
2.2. LCMV Infection
2.3. Monoclonal Antibody Neutralisation of CD8, Ly6G and PD-L1
2.4. RNA Extraction and Real-Time Quantitative Polymerase Chain Reaction
2.5. RNase Protection Assay
2.6. Histology of Liver and Lung Tissue
2.7. Quantification of Plasma Cytokines
2.8. Flow Cytometry
2.9. tSNE Analysis
2.10. Statistical Analysis
3. Results
3.1. Absence of IFNAR1 and PD-L1 Signalling Results in Lethal Disease following LCMV Infection
3.2. IFNAR1-Deficient Mice Fail to Accumulate LCMV-Specific CD8+ T Cells Independently of PD-L1
3.3. Ablation of IFNAR1 and PD-L1 Results in Enhanced Lung Pathology Following LCMV Infection
3.4. CD8+ T Cell Depletion but Not Neutrophil Depletion Rescues IFNAR1 x PD-L1 DKO Mice from Lethal LCMV Infection
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
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Spiteri, A.G.; Suprunenko, T.; Cutts, E.; Suen, A.; Ashhurst, T.M.; Viengkhou, B.; King, N.J.C.; Hofer, M.J. CD8+ T Cells Mediate Lethal Lung Pathology in the Absence of PD-L1 and Type I Interferon Signalling following LCMV Infection. Viruses 2024, 16, 390. https://doi.org/10.3390/v16030390
Spiteri AG, Suprunenko T, Cutts E, Suen A, Ashhurst TM, Viengkhou B, King NJC, Hofer MJ. CD8+ T Cells Mediate Lethal Lung Pathology in the Absence of PD-L1 and Type I Interferon Signalling following LCMV Infection. Viruses. 2024; 16(3):390. https://doi.org/10.3390/v16030390
Chicago/Turabian StyleSpiteri, Alanna G., Tamara Suprunenko, Erin Cutts, Andrew Suen, Thomas M. Ashhurst, Barney Viengkhou, Nicholas J. C. King, and Markus J. Hofer. 2024. "CD8+ T Cells Mediate Lethal Lung Pathology in the Absence of PD-L1 and Type I Interferon Signalling following LCMV Infection" Viruses 16, no. 3: 390. https://doi.org/10.3390/v16030390
APA StyleSpiteri, A. G., Suprunenko, T., Cutts, E., Suen, A., Ashhurst, T. M., Viengkhou, B., King, N. J. C., & Hofer, M. J. (2024). CD8+ T Cells Mediate Lethal Lung Pathology in the Absence of PD-L1 and Type I Interferon Signalling following LCMV Infection. Viruses, 16(3), 390. https://doi.org/10.3390/v16030390