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Correction

Correction: Stante et al. Four Novel Caudoviricetes Bacteriophages Isolated from Baltic Sea Water Infect Colonizers of Aurelia aurita. Viruses 2023, 15, 1525

1
Institute for General Microbiology, Christian Albrechts University, Am Botanischen Garten 1-9, D-24118 Kiel, Germany
2
Central Microscopy Facility, Christian Albrechts University, Am Botanischen Garten 1-9, D-24118 Kiel, Germany
*
Author to whom correspondence should be addressed.
Viruses 2024, 16(12), 1880; https://doi.org/10.3390/v16121880
Submission received: 17 October 2024 / Accepted: 22 October 2024 / Published: 4 December 2024
(This article belongs to the Section Bacterial Viruses)

Figure/Table Legend

In the original publication [1], there was a mistake in the legend for Figures 1 and 2 and Table 4. The host of the phage KMM1 is a Staphylococcus and not a Pseudomonas, as stated initially. Staphylococcus sp. has a new NCBI accession number (PQ151711). The correct legend appears below.
  • Figure 1. Plaque and virion morphology of isolated bacteriophages KMM1-KMM4. (A) Plaque morphologies were detected on MB double-agar layer plates after 16 h of incubation at 30 °C. Plaques formed on a lawn of Staphylococcus sp., PQ151711 (KMM1), Citrobacter freundii, OQ398153 (KMM2), and Citrobacter sp., OQ398154 (KMM3, KMM4). Scale bars represent 1 mm. (B) Transmission electron micrographs of phage lysates KMM1–KMM4, scale bars represent 50 nm.
  • Figure 2. Infection cycles of isolated phages. One-step growth curves over 120 min were performed to calculate the latent period (green arrow) and burst size (orange arrow). (A) KMM1-infected Staphylococcus sp. (PQ151711) after 27 min with the release of 55 pfu/cell, (B) KMM2-infected Citrobacter freundii (OQ398153) after 20 min with the release of 280 pfu/cell, (C) KMM3- and (D) KMM4-infected Citrobacter sp. (OQ398154) after 45 and 30 min, respectively, with the release of 60 and 120 pfu/cell, respectively. Values represent the mean of three biological replicates.
  • Table 4. Adsorption dynamics of bacteriophages KMM1–KMM4. Adsorption rates were determined 5 min after phage addition to the primary hosts (Staphylococcus sp., Citrobacter freundii, and Citrobacter sp.). The number of phages adsorbed to the cells generated a decrease in phage titer. The percentage of adsorbed phages and the adsorption constant (k) were calculated. Values are the mean of three biological replicates with corresponding standard deviations.

Error in Figure/Table

In the original publication, there was a mistake in the graphical abstract as published. Initially, the host of the KMM1 phage was reported as a Pseudomonas strain, based on a 97% sequence identity from a preliminary genetic analysis. However, after conducting more extensive tests at the DSMZ using MALDI-TOF, 16S rRNA sequencing, and microscopy, we confirmed that the correct host is a Staphylococcus strain, with a 99% sequence identity. The corrected graphical abstract appears below.
Viruses 16 01880 i001
  • Graphical abstract
In the original publication, there was a mistake in Table 1 as published. Strains No. 8, 9, and 170, after being re-sequenced, were found to be Staphylococcus spp. In addition, isolate No. 8 has a new NCBI accession number (PQ151711). The corrected Table 1 appears below.
In the original publication, there was a mistake in Table 2 as published. Initially, the host of the KMM1 phage was reported as a Pseudomonas strain, based on a 97% sequence identity from preliminary genetic analysis. However, after conducting more extensive tests at the DSMZ using MALDI-TOF, 16S rRNA sequencing, and microscopy, we confirmed that the correct host is a Staphylococcus strain, with a 99% sequence identity. We modified the name of the phage in Staphylococcus phage BSwM KMM1. The corrected Table 2 appears below.
In the original publication, there was a mistake in Figure 3 as published. After re-sequence, strains 8 (NCBI Accession No. PQ151711), 9 (NCBI Accession No. OQ398155), and 170 (NCBI Accession No. OQ398167) were moved to the other Staphylococcus strains. The corrected Figure 3 appears below.
In the original publication, there was a mistake in Figure 4 as published. The wrong KMM1 phage name was used. Phage KMM1’s name has been replaced with Staphylococcus phage BSwM KMM1. The corrected Figure 4 appears below.
Additionally, we would like to indicate that we made the following changes in the text of the manuscript—We replaced the name of “Pseudomonas phage BSwM KMM1” with “Staphylococcus phage BSwM KMM1” in all sections of the manuscript.
In the original publication, there was an error in identifying the primary host of phage KMM1. Initially, the host of phage KMM1 was reported to be a Pseudomonas strain based on 97% sequence identity obtained from preliminary genetic analysis. However, after conducting more extensive testing at DSMZ using MALDI-TOF, 16S rRNA sequencing and microscopy, we confirmed that the correct host was a Staphylococcus strain, with a sequence identity of 99%. The primary host of the Staphylococcus BSwM phage KMM1 is Staphylococcus aureus (strain No. 8 listed in Table 1) and has the following NCBI accession number (PQ151711), which was included in all sections of the manuscript. The corrected Section 3.4 was as follows:

3.4. All Isolated Phages Are Highly Specific and Effective

The KMM1 phage was initially found to infect Staphylococcus sp., while KMM2, KMM3, and KMM4 were shown to infect Citrobacter spp., which are phylogenetically classified in the Staphylococcaceae and Enterobacteriaceae, respectively. The host range of the phages was determined by spot assays on 43 strains (Table 1, column “Use in the study”, category “Host range”) belonging to the same genera, Staphylococcus and Citrobacter.
Furthermore, phages were tested against representatives of Pseudomonadaceae, Enterobacteraceae and Rhodobacteraceae of phylum Proteobacteria, Streptococcaceae of Bacilli, and Chryseobacterium, Olleya, and Maribacter of the abundant class of Flavobacteriia present in the A. aurita-associated microbiota. Bacterial sensitivity to a given bacteriophage was evaluated based on the occurrence of a lysis halo. Additionally, the respective phage efficiency of plating (EOP) was determined with those bacteria showing lysis in the spot tests. EOP for each host bacterium was calculated by comparing it with a score of 109 pfu/mL obtained for the original host infection. As shown by the heatmap in Figure 3, KMM1 infects, in addition to the primary host, 15 additional strains of the Gram-positive family Staphylococcaceae, two of them even with a slightly higher EOP. The phages KMM2, KMM3, and KMM4 showed comparable and narrow host ranges within the genus Citrobacter. However, the observed phage titers and EOP were different as indicated by the color-coding dependent on the value (Figure 3). Phages KMM2 and KMM4 were further able to infect the Enterobacteriaceae bacterium Shigella flexneri. In contrast, phage KMM3 infected two Escherichia coli strains of Enterobacteriaceae. The phages infected none of the Flavobacteriia representatives.
  • The corrected paragraph 2–5 of Section 4 was as follows:
In this study, four phages (Staphylococcus phage KMM1, Citrobacter phages KMM2, KMM3, and KMM4) were isolated from the Baltic Sea water column (Kiel fjord) surrounding A. aurita individuals by a cultivation-based approach, infecting previously isolated bacteria, Staphylococcus and Citrobacter, both present in the associated microbiota of A. aurita [21,22]. Phages KMM1, KMM2, and KMM4 showed a clear, roundish plaque morphology, as previously described for most Caudoviricetes with long contractile tails (formerly known as Myovirus-like phages) [103]. Phage KMM3, on the other hand, showed larger plaques with a clear center surrounded by a turbid halo, commonly referred to as a “bull’s eye” plaque [104,105]. The clear halo in the plaque’s center represents the phage’s lytic activity. The turbid ring surrounding the clear halo is formed by accumulating uninfected or partially infected host bacterial cells. These cells can resist phage infection (acquired resistance, defense systems) or have only been partially infected, potentially based on the aging of the bacterial lawn (non-infective after log phase), associated increases in the size of microcolonies making up the bacterial lawn, or because of less general phenomena such as the lysis inhibition phenotype [51,106]. However, it is important to note that phage plaque morphology can vary depending on the specific phage–host system and experimental conditions [107,108].
Citrobacter spp., classified as Gram-negative bacteria, are widely distributed in marine environments, including seawater, sediments, and marine eukaryotes [109–112]. Although little is known about the specific ecological roles of Citrobacter in marine environments, they are known to contribute to the degradation of organic matter [113]. Staphylococcus species in marine habitats play roles in various biological processes, such as biofilm formation and interactions with other marine microbes, and can indirectly contribute to nutrient cycling [114–116]. These bacteria often serve as indicators of pollution and pose public health risks, particularly due to their potential to harbor antibiotic resistance [117–119]. Frequently introduced into marine environments by human activities, both Staphylococcus and Citrobacter can persist and spread, impacting both ecosystem and human health [120,121]. As opportunistic pathogens, they can also cause infections in marine multicellular organisms [110,111,114,122,123]. However, Staphylococcus and Citrobacter species are generally not considered critical for the health of marine ecosystems [124,125]. Their presence in marine habitats is often linked to runoff or sewage discharge from human activities, rather than any significant ecological functions within the marine environment [126,127]. Consequently, virulent phages that infect these bacteria might help balance ecosystem and metaorganism homeostasis. Bacteriophages that target Staphylococcus and Citrobacter have been identified in marine environments and may play important roles in regulating bacterial populations [2,128]. These phages can control the abundance of their host bacteria, potentially limiting the spread of pathogenic or contaminant strains like Staphylococcus aureus and Citrobacter species [2,129]. By modulating the population sizes of these bacteria, phages contribute to microbial community dynamics, influence nutrient cycling, and help maintain ecological balance in marine ecosystems [129].
Moreover, the four isolated phages reflect narrow host range phages, infecting only a limited number of bacterial strains or species [80,130]. This specificity allows them to modulate bacterial populations by selectively limiting certain strains, such as S. aureus, which can otherwise spread uncontrollably and pose risks to marine ecosystems [131]. This targeted regulation is crucial for maintaining ecological balance and preventing the proliferation of potential pathogens [132]. Additionally, these phages can influence horizontal gene transfer by facilitating or inhibiting the movement of genetic material between bacteria, thereby affecting the spread of antibiotic resistance genes [133]. Due to these capabilities, narrow host range phages are valuable not only in natural ecosystems but also as potential tools in phage therapy to combat resistant infections [134,135]. Furthermore, their specificity makes them ideal candidates for bioengineering applications, such as designing targeted antimicrobials or developing biosensors [132,135,136].
All phages identified in this study showed effective and efficient lysis of Staphylococcus and Citrobacter by fast and effective binding of the phage to the host cells, short latency periods, and high burst sizes (Tables 3 and 4). These characteristics are further important features for affecting natural microbiomes and are particularly relevant for potential therapeutic applications. Phage therapy uses intact natural phages or phage compounds to treat bacterial infections [137]. Due to the growing number of antibiotic-resistant bacterial species and the ban on the use of antibiotics in the aquatic environment [78,138–140], the interest in phage therapy particularly for aquaculture increased during the last few decades [141–143]. Phage therapy relies on extraordinary qualities of phages, including host specificity, self-replication, wide distribution, and safety [43,137,142,144]. Since phages are a natural way of managing bacterial infections, their usage does not contribute to the development of antibiotic resistance or the deposition of harmful residues in the environment. Finally, phages are versatile since they may be used alone or in cooperation with antibiotics or other therapies to improve their potency against bacterial infections. These features are entirely applicable in aquaculture, where traditional approaches to deal with pathogenic bacteria, such as antibiotics, are impossible [145,146]. Building on these advantages, the narrow host range of the isolated KMM1-KMM4 phages offers targeted therapeutic options in aquaculture, where specific bacterial infections require precise management [147,148]. These phages are effective against particular strains of Staphylococcus or Citrobacter, allowing for focused intervention without affecting non-target bacteria [129]. Their ability to selectively infect and reduce populations of specific pathogens minimizes the risk of disrupting beneficial microbial communities [136,149]. However, for these phages to be practical in aquaculture, their effectiveness must be verified against further various bacterial strains, and their stability, safety, and cost-effective production under various environmental conditions, such as pH and temperature, need to be thoroughly evaluated.

References

Some parts of the text have been added, moved, and/or revised, and we included appropriate citations, with new numbered references [114–121,123–125,128,129,131–136,147–150]. With this correction, the order of some references has been adjusted accordingly.
114.
Shineh, G.; Mobaraki, M.; Perves Bappy, M.J.; Mills, D.K. Biofilm formation, and related impacts on healthcare, food processing and packaging, industrial manufacturing, marine industries, and sanitation—A review. Appl. Microbiol. 2023, 3, 629–665.
115.
Zammuto, V.; Rizzo, M.G.; Spano, A.; Spagnuolo, D.; Di Martino, A.; Morabito, M.; Manghisi, A.; Genovese, G.; Guglielmino, S.; Calabrese, G. Effects of crude polysaccharides from marine macroalgae on the adhesion and biofilm formation of Pseudomonas aeruginosa and Staphylococcus aureus. Algal Res. 2022, 63, 102646.
116.
Sentenac, H.; Loyau, A.; Leflaive, J.; Schmeller, D.S. The significance of biofilms to human, animal, plant and ecosystem health. Funct. Ecol. 2022, 36, 294–313.
117.
Singh, A.K.; Kaur, R.; Verma, S.; Singh, S. Antimicrobials and antibiotic resistance genes in water bodies: Pollution, risk, and control. Front. Environ. Sci. 2022, 10, 830861.
118.
Jampani, M.; Mateo-Sagasta, J.; Chandrasekar, A.; Fatta-Kassinos, D.; Graham, D.W.; Gothwal, R.; Moodley, A.; Chadag, V.M.; Wiberg, D.; Langan, S. Fate and transport modelling for evaluating antibiotic resistance in aquatic environments: Current knowledge and research priorities. J. Hazard. Mater. 2023, 461, 132527.
119.
Lajqi Berisha, N.; Poceva Panovska, A.; Hajrulai-Musliu, Z. Antibiotic Resistance and Aquatic Systems: Importance in Public Health. Water 2024, 16, 2362.
120.
Sarkar, S.; Kamle, M.; Bharti, A.; Kumar, P. Antibiotic-resistant bacteria risks and challenges for human health and environment: An overview. World J. Environ. Biosci. 2023, 12, 26–34.
121.
Ferheen, I.; Spurio, R.; Marcheggiani, S. Vehicle transmission of antibiotic-resistant pathogens mediated by plastic debris in aquatic ecosystems. iScience 2024, 27, 110026.
123.
Schilcher, K.; Horswill, A.R. Staphylococcal biofilm development: Structure, regulation, and treatment strategies. Microbiol. Mol. Biol. Rev. 2020, 84, 10–1128.
124.
Nogales, B.; Lanfranconi, M.P.; Piña-Villalonga, J.M.; Bosch, R. Anthropogenic perturbations in marine microbial communities. FEMS Microbiol. Rev. 2011, 35, 275–298.
125.
Gambino, D.; Vicari, D.; Vitale, M.; Schirò, G.; Mira, F.; Giglia, M.L.; Riccardi, A.; Gentile, A.; Giardina, S.; Carrozzo, A. Study on bacteria isolates and antimicrobial resistance in wildlife in Sicily, southern Italy. Microorganisms 2021, 9, 203.
128.
Royam, M.M.; Nachimuthu, R. Isolation, characterization, and efficacy of bacteriophages isolated against Citrobacter spp. an in vivo approach in a zebrafish model (Danio rerio). Res. Microbiol. 2020, 171, 341–350.
129.
Castledine, M.; Buckling, A. Critically evaluating the relative importance of phage in shaping microbial community composition. Trends Microbiol. 2024, 32, 957–969.
131.
Santos, J.D.; Vitorino, I.; Reyes, F.; Vicente, F.; Lage, O.M. From ocean to medicine: Pharmaceutical applications of metabolites from marine bacteria. Antibiotics 2020, 9, 455.
132.
García, P.; Madera, C.; Martinez, B.; Rodríguez, A.; Suárez, J.E. Prevalence of bacteriophages infecting Staphylococcus aureus in dairy samples and their potential as biocontrol agents. J. Dairy Sci. 2009, 92, 3019–3026.
133.
Sala-Comorera, L.; Nolan, T.M.; Reynolds, L.J.; Venkatesh, A.; Cheung, L.; Martin, N.A.; Stephens, J.H.; Gitto, A.; O’Hare, G.M.; O’Sullivan, J.J. Bacterial and bacteriophage antibiotic resistance in marine bathing waters in relation to rivers and urban streams. Front. Microbiol. 2021, 12, 718234.
134.
Blanco-Picazo, P.; Roscales, G.; Toribio-Avedillo, D.; Gómez-Gómez, C.; Avila, C.; Ballesté, E.; Muniesa, M.; Rodríguez-Rubio, L. Antibiotic resistance genes in phage particles from Antarctic and Mediterranean seawater ecosystems. Microorganisms 2020, 8, 1293.
135.
Liu, K.; Wang, C.; Zhou, X.; Guo, X.; Yang, Y.; Liu, W.; Zhao, R.; Song, H. Bacteriophage therapy for drug-resistant Staphylococcus aureus infections. Front. Cell. Infect. Microbiol. 2024, 14, 1336821.
136.
Petrovic Fabijan, A.; Lin, R.C.; Ho, J.; Maddocks, S.; Ben Zakour, N.L.; Iredell, J.R. Safety of bacteriophage therapy in severe Staphylococcus aureus infection. Nat. Microbiol. 2020, 5, 465–472.
147.
Rai, S.; Kaur, B.; Singh, P.; Singh, A.; Benjakul, S.; Vijay Kumar Reddy, S.; Nagar, V.; Tyagi, A. Perspectives on phage therapy for health management in aquaculture. Aquac. Int. 2024, 32, 1349–1393.
148.
Lomelí-Ortega, C.O.; Balcázar, J.L.; Quiroz-Guzmán, E. Phage therapy and aquaculture: Progress and challenges. Int. Microbiol. 2023, 26, 439–441.
149.
Strathdee, S.A.; Hatfull, G.F.; Mutalik, V.K.; Schooley, R.T. Phage therapy: From biological mechanisms to future directions. Cell 2023, 186, 17–31.
150.
Schackart, K.E., III; Graham, J.B.; Ponsero, A.J.; Hurwitz, B.L. Evaluation of computational phage detection tools for metagenomic datasets. Front. Microbiol. 2023, 14, 1078760.
In addition, there are some words and content modifications throughout the text. The content of the Supplementary Materials has also been changed accordingly. The authors state that the scientific conclusions are unaffected. This correction was approved by the Academic Editor. The original publication has also been updated.

Reference

  1. Stante, M.; Weiland-Bräuer, N.; Repnik, U.; Werner, A.; Bramkamp, M.; Chibani, C.M.; Schmitz, R.A. Four Novel Caudoviricetes Bacteriophages Isolated from Baltic Sea Water Infect Colonizers of Aurelia aurita. Viruses 2023, 15, 1525. [Google Scholar] [CrossRef] [PubMed]
Figure 3. Host range of isolated phages. Phages KMM1–KMM4 were used for infection assays with selected taxons (color code on the right categorizes taxons into classes). The efficiency of plating (EOP) for each host bacterium was calculated by comparing it with a score of 109 pfu/mL for the original host infection (value = 1). Missing coloring indicates no infection.
Figure 3. Host range of isolated phages. Phages KMM1–KMM4 were used for infection assays with selected taxons (color code on the right categorizes taxons into classes). The efficiency of plating (EOP) for each host bacterium was calculated by comparing it with a score of 109 pfu/mL for the original host infection (value = 1). Missing coloring indicates no infection.
Viruses 16 01880 g003
Figure 4. Taxonomic classification of phages KMM1–KMM4. Phylogenetic tree of isolated phages KMM1–KMM4 (red rectangles) was generated with the whole genome-based VICTOR analysis. Phages belonging to different families, subfamilies, genera, and species were color coded. The scale represents homology in %.
Figure 4. Taxonomic classification of phages KMM1–KMM4. Phylogenetic tree of isolated phages KMM1–KMM4 (red rectangles) was generated with the whole genome-based VICTOR analysis. Phages belonging to different families, subfamilies, genera, and species were color coded. The scale represents homology in %.
Viruses 16 01880 g004
Table 1. Bacterial strains used in this study. Bacterial strains were isolated in the study [22]. Isolates are sorted by the last column and phylum level. The column “Use in This study” refers to the use of the strains in the study. If not stated differently, the listed numbers in column “Reference” reflect NCBI Accession Numbers.
Table 1. Bacterial strains used in this study. Bacterial strains were isolated in the study [22]. Isolates are sorted by the last column and phylum level. The column “Use in This study” refers to the use of the strains in the study. If not stated differently, the listed numbers in column “Reference” reflect NCBI Accession Numbers.
Strain
No.
StrainReferencePhylumClassOrderFamilySourceGrowth MediumGrowth Temp.Use in This Study
74Micrococcus luteusMK967048.1ActinomycetotaActinomycetiaMicrococcalesMicrococcaceaeA. aurita polyp Baltic Sea husbandryMarine Bouillon30 °Cenrichment/
first screening
75Arthrobacter sp. MK967049.1ActinomycetotaActinomycetiaMicrococcalesMicrococcaceaeA. aurita polyp Baltic Sea husbandryMarine Bouillon30 °Cenrichment/
first screening
83Gordonia terraeMK967057.1ActinomycetotaActinomycetiaMycobacterialesGordoniaceaeA. aurita polyp Baltic Sea husbandryMarine Bouillon30 °Cenrichment/
first screening
15Sulfitobacter sp. MK967015.1PseudomonadotaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeA. aurita medusa Baltic SeaMarine Bouillon30 °Cenrichment/
first screening
20Sulfitobacter pontiacusMK967020.1PseudomonadotaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeA. aurita medusa Baltic Sea husbandryMarine Bouillon30 °Cenrichment/
first screening
23Sulfitobacter sp. MK967023.1PseudomonadotaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeA. aurita medusa Baltic Sea husbandryMarine Bouillon30 °Cenrichment/
first screening
69Rhodobacter sp.MK967043.1PseudomonadotaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeM. leidyi Baltic Sea husbandryMarine Bouillon30 °Cenrichment/
first screening
78Sulfitobacter sp.MK967052.1PseudomonadotaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeA. aurita polyp Baltic Sea husbandryMarine Bouillon30 °Cenrichment/
first screening
86Ruegeria sp.MK967060.1PseudomonadotaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeA. aurita polyp Baltic Sea husbandryMarine Bouillon30 °Cenrichment/
first screening
89Ruegeria sp. MK967063.1PseudomonadotaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeA. aurita polyp Baltic Sea husbandryMarine Bouillon30 °Cenrichment/
first screening
100Sulfitobacter sp.MK967074.1PseudomonadotaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeA. aurita polyp North Sea husbandryMarine Bouillon30 °Cenrichment/
first screening
117Ruegeria mobilisMK967091.1PseudomonadotaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeA. aurita polyp North Atlantic husbandryMarine Bouillon30 °Cenrichment/
first screening
147Phaeobacter gallaeciensisMK967120.1PseudomonadotaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeArtificial Seawater 18 PSUMarine Bouillon30 °Cenrichment/
first screening
188Sulfitobacter pseudonitzschiaeMK967160.1PseudomonadotaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeArtificial Seawater 30 PSUMarine Bouillon30 °Cenrichment/
first screening
13Bacillus cereusMK967013.1BacillotaBacilliBacillalesBacillaceaeA. aurita medusa Baltic SeaMarine Bouillon30 °Cenrichment/
first screening
16Bacillus sp.MK967016.1BacillotaBacilliBacillalesBacillaceaeA. aurita medusa Baltic SeaMarine Bouillon30 °Cenrichment/
first screening
17Bacillus cereusMK967017.1BacillotaBacilliBacillalesBacillaceaeA. aurita medusa Baltic SeaMarine Bouillon30 °Cenrichment/
first screening
19Bacillus sp.MK967019.1BacillotaBacilliBacillalesBacillaceaeA. aurita medusa Baltic Sea husbandryMarine Bouillon30 °Cenrichment/
first screening
76Bacillus weihenstephanensisMK967050.1BacillotaBacilliBacillalesBacillaceaeA. aurita polyp Baltic Sea husbandryMarine Bouillon30 °Cenrichment/
first screening
85Staphylococcus warneriMK967059.1BacillotaBacilliBacillalesStaphylococcaceaeA. aurita polyp Baltic Sea husbandryMarine Bouillon30 °Cenrichment/
first screening
88Staphylococcus sp.MK967062.1BacillotaBacilliBacillalesStaphylococcaceaeA. aurita polyp Baltic Sea husbandryMarine Bouillon30 °Cenrichment/
first screening
73Enterococcus casseliflavusMK967047.1BacillotaBacilliLactobacillalesEnterococcaceaeA. aurita polyp Baltic Sea husbandryMarine Bouillon30 °Cenrichment/
first screening
24Maribacter sp.MK967024.1BacteroidotaFlavobacteriiaFlavobacterialesFlavobacteriaceaeA. aurita medusa Baltic Sea husbandryMarine Bouillon30 °Cenrichment/
first screening
57Olleya marilimosaMK967032.1BacteroidotaFlavobacteriiaFlavobacterialesFlavobacteriaceaeM. leidyi Baltic SeaMarine Bouillon30 °Cenrichment/
first screening
79Olleya sp. MK967053.1BacteroidotaFlavobacteriiaFlavobacterialesFlavobacteriaceaeA. aurita polyp Baltic Sea husbandryMarine Bouillon30 °Cenrichment/
first screening
181Chryseobacterium sp.MK967154.1BacteroidotaFlavobacteriiaFlavobacterialesWeeksellaceaeArtificial Seawater 30 PSUMarine Bouillon30 °Cenrichment/
first screening
257Chryseobacterium sp.MK967218.1BacteroidotaFlavobacteriiaFlavobacterialesWeeksellaceaeM. leidyi Baltic Sea husbandryMarine Bouillon30 °Cenrichment/
first screening
22Pseudolateromonas sp.MK967022.1PseudomonadotaGammaproteobacteriaAlteromonadalesPseudoalteromonadaceaeA. aurita medusa Baltic Sea husbandryMarine Bouillon30 °Cenrichment/
first screening
91Pseudoalteromonas prydzensisMK967065.1PseudomonadotaGammaproteobacteriaAlteromonadalesPseudoalteromonadaceaeA. aurita polyp Baltic Sea husbandryMarine Bouillon30 °Cenrichment/
first screening
101Pseudoalteromonas issachenkoniiMK967075.1PseudomonadotaGammaproteobacteriaAlteromonadalesPseudoalteromonadaceaeA. aurita polyp North Sea husbandryMarine Bouillon30 °Cenrichment/
first screening
167Pseudoalteromonas sp.MK967140.1PseudomonadotaGammaproteobacteriaAlteromonadalesPseudoalteromonadaceaeArtificial Seawater 18 PSUMarine Bouillon30 °Cenrichment/
first screening
203Pseudoalteromonas espejianaMK967174.1PseudomonadotaGammaproteobacteriaAlteromonadalesPseudoalteromonadaceaeArtificial Seawater 30 PSUMarine Bouillon30 °Cenrichment/
first screening
219Pseudoalteromonas tunicataMK967188.1PseudomonadotaGammaproteobacteriaAlteromonadalesPseudoalteromonadaceaeM. leidyi Baltic SeaMarine Bouillon30 °Cenrichment/
first screening
224Pseudoalteromonas lipolyticaMK967191.1PseudomonadotaGammaproteobacteriaAlteromonadalesPseudoalteromonadaceaeM. leidyi Baltic SeaMarine Bouillon30 °Cenrichment/
first screening
105Shewanella basaltisMK967079.1PseudomonadotaGammaproteobacteriaAlteromonadalesShewanellaceaeA. aurita polyp North Sea husbandryMarine Bouillon30 °Cenrichment/
first screening
21Cobetia amphilectiMK967021.1PseudomonadotaGammaproteobacteriaOceanospirillalesHalomonadaceaeA. aurita medusa Baltic Sea husbandryMarine Bouillon30 °Cenrichment/
first screening
55Marinomonas hwangdonensisMK967030.1PseudomonadotaGammaproteobacteriaOceanospirillalesOceanospirillaceaeM. leidyi Baltic SeaMarine Bouillon30 °Cenrichment/
first screening
222Marinomonas ponticaMK967189.1PseudomonadotaGammaproteobacteriaOceanospirillalesOceanospirillaceaeM. leidyi Baltic SeaMarine Bouillon30 °Cenrichment/
first screening
262Oceanospirillaceae bacteriumMK967222.1PseudomonadotaGammaproteobacteriaOceanospirillalesOceanospirillaceaeM. leidyi Baltic SeaMarine Bouillon30 °Cenrichment/
first screening
11Pseudomonas sp.MK967012.1PseudomonadotaGammaproteobacteriaPseudomonadalesPseudomonadaceaeA. aurita medusa Baltic SeaMarine Bouillon30 °Cenrichment/
first screening
90Pseudomonas putidaMK967064.1PseudomonadotaGammaproteobacteriaPseudomonadalesPseudomonadaceaeA. aurita polyp Baltic Sea husbandryMarine Bouillon30 °Cenrichment/
first screening
92Pseudomonas putidaMK967066.1PseudomonadotaGammaproteobacteriaPseudomonadalesPseudomonadaceaeA. aurita polyp Baltic Sea husbandryMarine Bouillon30 °Cenrichment/
first screening
93Pseudomonas sp.MK967067.1PseudomonadotaGammaproteobacteriaPseudomonadalesPseudomonadaceaeA. aurita polyp Baltic Sea husbandryMarine Bouillon30 °Cenrichment/
first screening
94Pseudomonas sp.MK967068.1PseudomonadotaGammaproteobacteriaPseudomonadalesPseudomonadaceaeA. aurita polyp Baltic Sea husbandryMarine Bouillon30 °Cenrichment/
first screening
132Pseudomonas fluorescensMK967106.1PseudomonadotaGammaproteobacteriaPseudomonadalesPseudomonadaceaeArtificial Seawater 18 PSUMarine Bouillon30 °Cenrichment/
first screening
196Pseudomonas syringaeMK967168.1PseudomonadotaGammaproteobacteriaPseudomonadalesPseudomonadaceaeArtificial Seawater 30 PSUMarine Bouillon30 °Cenrichment/
first screening
77Vibrio anguillarumMK967051.1PseudomonadotaGammaproteobacteriaVibrionalesVibrionaceaeA. aurita polyp Baltic Sea husbandryMarine Bouillon30 °Cenrichment/
first screening
80Vibrio anguillarumMK967054.1PseudomonadotaGammaproteobacteriaVibrionalesVibrionaceaeA. aurita polyp Baltic Sea husbandryMarine Bouillon30 °Cenrichment/
first screening
18Staphylococcus aureusOQ398157BacillotaBacilliBacillalesStaphylococcaceaeA. aurita medusa Baltic Sea husbandryMarine Bouillon30 °Cenrichment/
first screening
134Staphylococcus aureusOQ398164BacillotaBacilliBacillalesStaphylococcaceaeArtificial Seawater 18 PSUMarine Bouillon30 °Cenrichment/
first screening
87Staphylococcus aureusOQ398160BacillotaBacilliBacillalesStaphylococcaceaeA. aurita polyp Baltic Sea husbandryMarine Bouillon30 °Cenrichment/
first screening
14Staphylococcus warneriOQ398156BacillotaBacilliBacillalesStaphylococcaceaeA. aurita medusa Baltic SeaMarine Bouillon30 °Cenrichment/
first screening
6Citrobacter freundiiOQ398153PseudomonadotaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeA. aurita medusa Baltic SeaMarine Bouillon30 °Cenrichment/
first screening/
host range
7Citrobacter sp.OQ398154PseudomonadotaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeA. aurita medusa Baltic SeaMarine Bouillon30 °Cenrichment/
first screening/
host range
8Staphylococcus aureusPQ151711BacillotaBacilliBacillalesStaphylococcaceaeA. aurita medusa Baltic SeaMarine Bouillon30 °Cenrichment/
first screening/
host range
62Sulfitobacter pontiacusOQ398158PseudomonadotaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeM. leidyi Baltic SeaMarine Bouillon30 °Chost range
97Shewanella sp. OQ398161PseudomonadotaGammaproteobacteriaAlteromonadalesShewanellaceaeA. aurita polyp North Sea husbandryMarine Bouillon30 °Chost range
199Staphylococcus aureusOQ398168BacillotaBacilliBacillalesStaphylococcaceaeArtificial Seawater 30 PSUMarine Bouillon30 °Chost range
DSMZ 11823Staphylococcus aureusDSMZ 11823BacillotaBacilliBacillalesStaphylococcaceaeclinical materialTrypticase Soy Yeast Broth37 °Chost range
67Staphylococcus aureusOQ398159BacillotaBacilliBacillalesStaphylococcaceaeM. leidyi Baltic Sea husbandryMarine Bouillon30 °Chost range
102Staphylococcus aureusOQ398162BacillotaBacilliBacillalesStaphylococcaceaeA. aurita polyp North Sea husbandryMarine Bouillon30 °Chost range
158Staphylococcus aureusOQ398165BacillotaBacilliBacillalesStaphylococcaceaeArtificial Seawater 18 PSUMarine Bouillon30 °Chost range
161Staphylococcus aureusOQ398166BacillotaBacilliBacillalesStaphylococcaceaeArtificial Seawater 18 PSUMarine Bouillon30 °Chost range
127Staphylococcus aureusOQ398163BacillotaBacilliBacillalesStaphylococcaceaeA. aurita polyp North Atlantic husbandryMarine Bouillon30 °Chost range
DSMZ 28319Staphylococcus epidermidisDSMZ 28319BacillotaBacilliBacillalesStaphylococcaceaecatheter sepsisTrypticase Soy Yeast Broth37 °Chost range
DSMZ 20328Staphylococcus hominisDSMZ 20328BacillotaBacilliBacillalesStaphylococcaceaehuman skinTrypticase Soy Yeast Broth37 °Chost range
DSMZ 100616Staphylococcus warneriDSMZ 100616BacillotaBacilliBacillalesStaphylococcaceaecleanroom facility, TASTrypticase Soy Yeast Broth30 °Chost range
DSMZ 12643Streptococcus mitisDSMZ 12643BacillotaBacilliLactobacillalesStreptococcaceaeoral cavity, humanTrypticase Soy Yeast Broth37 °Chost range
DSMZ 20523Streptococcus mutansDSMZ 20523BacillotaBacilliLactobacillalesStreptococcaceaecarious dentineTrypticase Soy Yeast Broth37 °Chost range
296Citrobacter braakiiOQ398170PseudomonadotaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeA. aurita polyp North Atlantic husbandryMarine Bouillon30 °Chost range
283Citrobacter freundiiOQ398169PseudomonadotaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeA. aurita polyp Baltic Sea husbandryMarine Bouillon30 °Chost range
321Citrobacter freundiiOQ398172PseudomonadotaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeArtifical Seawater 30 PSUMarine Bouillon30 °Chost range
313Citrobacter sp.OQ398171PseudomonadotaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeArtifical Seawater 18 PSUMarine Bouillon30 °Chost range
DSMZ 18039Escherichia coliDSMZ 18039PseudomonadotaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeunknown sourceLuria-Bertani Bouillon37 °Chost range
strain 8Escherichia coli[47]PseudomonadotaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeunknown sourceLuria-Bertani Bouillon37 °Chost range
DSMZ 30083Escherichia coliDSMZ 30083PseudomonadotaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeurineLuria-Bertani Bouillon37 °Chost range
DSMZ 13698Escherichia fergusoniiDSMZ 13698PseudomonadotaGammaproteobacteriaEnterobacteralesEnterobacteriaceaefaeces of 1-year-old boyLuria-Bertani Bouillon37 °Chost range
strain 27Klebsiella oxytocaProf. Dr. Podschun, (National Reference Laboratory for Klebsiella species, Kiel University)PseudomonadotaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeunknown sourceNutrient Broth30 °Chost range
DSMZ
30104
Klebsiella pneumoniaeDSMZ 30104PseudomonadotaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeunknown sourceNutrient Broth30 °Chost range
DSMZ 4782Shigella flexeneriDSMZ 4782PseudomonadotaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeunknown sourceCaso Bouillon37 °Chost range
DSMZ 1707Pseudomonas aeruginosaDSMZ 1707PseudomonadotaGammaproteobacteriaPseudomonadalesPseudomonadaceaeunknown sourceCaso Bouillon30 °Chost range
9Staphylococcus aureusOQ398155BacillotaBacilliBacillalesStaphylococcaceaeA. aurita medusa Baltic SeaMarine Bouillon30 °Chost range
170Staphylococcus aureusOQ398167BacillotaBacilliBacillalesStaphylococcaceaeArtificial Seawater 18 PSUMarine Bouillon30 °Chost range
DSMZ 50256Pseudomonas syringaeDSMZ 50256PseudomonadotaGammaproteobacteriaPseudomonadalesPseudomonadaceaeTriticum aestivum, glume rot of wheatCaso Bouillon30 °Chost range
24Maribacter sp.MK967024.1BacteroidotaFlavobacteriiaFlavobacterialesFlavobacteriaceaeA. aurita medusa Baltic Sea husbandryMarine Bouillon30 °Chost range
79Olleya sp.MK967053.1BacteroidotaFlavobacteriiaFlavobacterialesFlavobacteriaceaeA. aurita polyp Baltic Sea husbandryMarine Bouillon30 °Chost range
98Olleya marilimosaMK967072.1BacteroidotaFlavobacteriiaFlavobacterialesFlavobacteriaceaeA. aurita polyp North Sea husbandryMarine Bouillon30 °Chost range
108Chryseobacterium hominisMK967082.1BacteroidotaFlavobacteriiaFlavobacterialesFlavobacteriaceaeA. aurita polyp North Sea husbandryMarine Bouillon30 °Chost range
57Olleya marilimosaMK967032.1BacteroidotaFlavobacteriiaFlavobacterialesFlavobacteriaceaeM. leidyi Baltic SeaMarine Bouillon30 °Chost range
199Maribacter sp. MK967170.1BacteroidotaFlavobacteriiaFlavobacterialesFlavobacteriaceaeArtificial Seawater 30 PSUMarine Bouillon30 °Chost range
Table 2. Viral genome characteristics and overview of assembly-related metrics.
Table 2. Viral genome characteristics and overview of assembly-related metrics.
PhageNCBI Accession No.No. of ReadsNo. of Filtered ReadsSequence CoverageN50Genome Length (bps)GC Content (%)Predicted ORFsUnknown Proteins
Staphylococcus phage BSwM KMM1OP9022944.0853.214247.48817.553137.38631.77259200
Citrobacter phage BSwM KMM2OP90229581059574.16322.11888.53739.5513794
Citrobacter phage BSwS KMM3OP902292837598130.67620.51749.16443.179258
Citrobacter phage BSwM KMM4OP9022936.4335.371544.32723.89486.91139.02138100
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Stante, M.; Weiland-Bräuer, N.; Repnik, U.; Werner, A.; Bramkamp, M.; Chibani, C.M.; Schmitz, R.A. Correction: Stante et al. Four Novel Caudoviricetes Bacteriophages Isolated from Baltic Sea Water Infect Colonizers of Aurelia aurita. Viruses 2023, 15, 1525. Viruses 2024, 16, 1880. https://doi.org/10.3390/v16121880

AMA Style

Stante M, Weiland-Bräuer N, Repnik U, Werner A, Bramkamp M, Chibani CM, Schmitz RA. Correction: Stante et al. Four Novel Caudoviricetes Bacteriophages Isolated from Baltic Sea Water Infect Colonizers of Aurelia aurita. Viruses 2023, 15, 1525. Viruses. 2024; 16(12):1880. https://doi.org/10.3390/v16121880

Chicago/Turabian Style

Stante, Melissa, Nancy Weiland-Bräuer, Urska Repnik, Almut Werner, Marc Bramkamp, Cynthia M. Chibani, and Ruth A. Schmitz. 2024. "Correction: Stante et al. Four Novel Caudoviricetes Bacteriophages Isolated from Baltic Sea Water Infect Colonizers of Aurelia aurita. Viruses 2023, 15, 1525" Viruses 16, no. 12: 1880. https://doi.org/10.3390/v16121880

APA Style

Stante, M., Weiland-Bräuer, N., Repnik, U., Werner, A., Bramkamp, M., Chibani, C. M., & Schmitz, R. A. (2024). Correction: Stante et al. Four Novel Caudoviricetes Bacteriophages Isolated from Baltic Sea Water Infect Colonizers of Aurelia aurita. Viruses 2023, 15, 1525. Viruses, 16(12), 1880. https://doi.org/10.3390/v16121880

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