Elimination of Solanum nigrum ilarvirus 1 and Apple Hammerhead Viroid from Apple Cultivars Using Antivirals Ribavirin, Rimantadine, and Zidovudine
Abstract
1. Introduction
2. Materials and Methods
2.1. In Vitro Cultures
2.2. Antivirals
2.3. Application of Antivirals
2.4. Plant Material & RNA Extraction
2.5. Diagnostics of SnIV-1 and AHVd by RT-PCR
2.6. Virus and Viroid Diagnostics by RT-qPCR
3. Results
3.1. Elimination of SnIV-1
3.2. Elimination of AHVd
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Target | Primer Name | Primer Sequence (5’-3’) | Usage; Region | Reference |
---|---|---|---|---|
Malus domestica, NADH a | 2277 | TGCTCCATGGATCTCATCGG | RT-qPCR, RT-PCR, RNA extraction and PCR amplification control; protein-coding region | [10] |
2278 | AATCGAGGGCTATGCGGATC | |||
AHVd b | 2115 | CTGCCGAAACAGAGGTTGGA | RT-qPCR, RT-PCR; genome | |
2116 | GAGAAGTCGCTCTCTCTCGC | |||
SnIV-1 c | 2696 | TTTGGGTTTGTAGCCGAATC | RT-qPCR; gene for coat protein | this work |
2697 | TTACCTCCGAGATCAACGTC | |||
2365 | GCCCTATCTCTACCCGAGGT | RT-PCR; gene for polymerase | ||
2363 | TGTCGAAGGACAGCCGAAAT |
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Sedlák, J.; Přibylová, J.; Koloňuk, I.; Špak, J.; Lenz, O.; Semerák, M. Elimination of Solanum nigrum ilarvirus 1 and Apple Hammerhead Viroid from Apple Cultivars Using Antivirals Ribavirin, Rimantadine, and Zidovudine. Viruses 2023, 15, 1684. https://doi.org/10.3390/v15081684
Sedlák J, Přibylová J, Koloňuk I, Špak J, Lenz O, Semerák M. Elimination of Solanum nigrum ilarvirus 1 and Apple Hammerhead Viroid from Apple Cultivars Using Antivirals Ribavirin, Rimantadine, and Zidovudine. Viruses. 2023; 15(8):1684. https://doi.org/10.3390/v15081684
Chicago/Turabian StyleSedlák, Jiří, Jaroslava Přibylová, Igor Koloňuk, Josef Špak, Ondřej Lenz, and Matěj Semerák. 2023. "Elimination of Solanum nigrum ilarvirus 1 and Apple Hammerhead Viroid from Apple Cultivars Using Antivirals Ribavirin, Rimantadine, and Zidovudine" Viruses 15, no. 8: 1684. https://doi.org/10.3390/v15081684
APA StyleSedlák, J., Přibylová, J., Koloňuk, I., Špak, J., Lenz, O., & Semerák, M. (2023). Elimination of Solanum nigrum ilarvirus 1 and Apple Hammerhead Viroid from Apple Cultivars Using Antivirals Ribavirin, Rimantadine, and Zidovudine. Viruses, 15(8), 1684. https://doi.org/10.3390/v15081684