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Article

Hyperimmunized Chickens Produce Neutralizing Antibodies against SARS-CoV-2

1
Department of Animal Science, College of Agricultural and Environmental Sciences, University of California-Davis, Davis, CA 95616, USA
2
School of Life Sciences, Faculty of Science, University of Technology Sydney, Sydney, NSW 2007, Australia
3
National Center for Biodefense and Infectious Diseases, George Mason University, Fairfax, VA 22030, USA
4
Department of Population Health and Reproduction, School of Veterinary Medicine, University of California-Davis, Davis, CA 95616, USA
*
Author to whom correspondence should be addressed.
Academic Editors: Lianpan Dai and Ahmed Elshamy
Viruses 2022, 14(7), 1510; https://doi.org/10.3390/v14071510
Received: 14 March 2022 / Revised: 24 June 2022 / Accepted: 7 July 2022 / Published: 9 July 2022
The novel severe acute respiratory syndrome (SARS) coronavirus, SARS-CoV-2, is responsible for the global COVID-19 pandemic. Effective interventions are urgently needed to mitigate the effects of COVID-19 and likely require multiple strategies. Egg-extracted antibody therapies are a low-cost and scalable strategy to protect at-risk individuals from SARS-CoV-2 infection. Commercial laying hens were hyperimmunized against the SARS-CoV-2 S1 protein using three different S1 recombinant proteins and three different doses. Sera and egg yolk were collected at three and six weeks after the second immunization for enzyme-linked immunosorbent assay and plaque-reduction neutralization assay to determine antigen-specific antibody titers and neutralizing antibody titers, respectively. In this study we demonstrate that hens hyperimmunized against the SARS-CoV-2 recombinant S1 and receptor binding domain (RBD) proteins produced neutralizing antibodies against SARS-CoV-2. We further demonstrate that antibody production was dependent on the dose and type of antigen administered. Our data suggests that antibodies purified from the egg yolk of hyperimmunized hens can be used as immunoprophylaxis in humans at risk of exposure to SARS-CoV-2. View Full-Text
Keywords: COVID-19; SARS-CoV-2; chicken; passive immunization; antibodies; neutralizing antibodies; egg COVID-19; SARS-CoV-2; chicken; passive immunization; antibodies; neutralizing antibodies; egg
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MDPI and ACS Style

Aston, E.J.; Wallach, M.G.; Narayanan, A.; Egaña-Labrin, S.; Gallardo, R.A. Hyperimmunized Chickens Produce Neutralizing Antibodies against SARS-CoV-2. Viruses 2022, 14, 1510. https://doi.org/10.3390/v14071510

AMA Style

Aston EJ, Wallach MG, Narayanan A, Egaña-Labrin S, Gallardo RA. Hyperimmunized Chickens Produce Neutralizing Antibodies against SARS-CoV-2. Viruses. 2022; 14(7):1510. https://doi.org/10.3390/v14071510

Chicago/Turabian Style

Aston, Emily J., Michael G. Wallach, Aarthi Narayanan, Sofia Egaña-Labrin, and Rodrigo A. Gallardo. 2022. "Hyperimmunized Chickens Produce Neutralizing Antibodies against SARS-CoV-2" Viruses 14, no. 7: 1510. https://doi.org/10.3390/v14071510

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