B-Cell-Epitope-Based Fluorescent Quantum Dot Biosensors for SARS-CoV-2 Enable Highly Sensitive COVID-19 Antibody Detection
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials and Chemicals
2.2. Serum Samples
2.3. Epitope Prediction and Polypeptide Synthesis
2.4. Homologous Analysis and Proteins Model
2.5. ELISA
2.6. Preparation of QDs Fluorescence Probes
2.7. TEM
2.8. Absorption Spectrum Measurement
2.9. FTIR
2.10. Detection of the Serum or Antibodies by Specific QDs
2.11. Statistical Analysis
3. Results
3.1. Characterization of the Fluorescent QD-Peptides Biosensor
3.2. QD-SARS-CoV-2 Peptides React with the Corresponding Antibody
3.3. Identification of Four Highly Sensitive B Cell Epitopes of SARS-CoV-2
3.4. QDs Coupled with Four Highly Sensitive SARS-CoV-2 Peptides Efficiently Respond to the Corresponding Antibody
3.5. Fluorescent QD-Peptides Biosensors Exhibit Excellent Clinical Sensitivity
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Severity of Illness | ELISA-SRBD | QD-Sepi1 | QD-Sepi2 | ELISA-N | QD-Nepi1 | QD-Nepi2 |
---|---|---|---|---|---|---|
Asymptomatic (6) | 45.08% | 49.47% | 51.36% | 60.35% | 55.19% | 62.25% |
Mild (87) | 42.68% | 37.97% | 43.46% | 69.12% | 47.76% | 50.63% |
Normal (67) | 42.34% | 28.86% | 24.92% | 66.34% | 42.00% | 45.49% |
Severe or critical (47) | 50.35% | 39.51% | 35.95% | 63.42% | 55.21% | 50.30% |
Overall (207) | 45.24% | 36.32% | 36.42% | 66.44% | 48.28% | 49.49% |
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Zheng, Y.; Song, K.; Cai, K.; Liu, L.; Tang, D.; Long, W.; Zhai, B.; Chen, J.; Tao, Y.; Zhao, Y.; et al. B-Cell-Epitope-Based Fluorescent Quantum Dot Biosensors for SARS-CoV-2 Enable Highly Sensitive COVID-19 Antibody Detection. Viruses 2022, 14, 1031. https://doi.org/10.3390/v14051031
Zheng Y, Song K, Cai K, Liu L, Tang D, Long W, Zhai B, Chen J, Tao Y, Zhao Y, et al. B-Cell-Epitope-Based Fluorescent Quantum Dot Biosensors for SARS-CoV-2 Enable Highly Sensitive COVID-19 Antibody Detection. Viruses. 2022; 14(5):1031. https://doi.org/10.3390/v14051031
Chicago/Turabian StyleZheng, Yucheng, Kun Song, Kun Cai, Linlin Liu, Dixiao Tang, Wenbo Long, Bohui Zhai, Jianjun Chen, Yanbing Tao, Yunong Zhao, and et al. 2022. "B-Cell-Epitope-Based Fluorescent Quantum Dot Biosensors for SARS-CoV-2 Enable Highly Sensitive COVID-19 Antibody Detection" Viruses 14, no. 5: 1031. https://doi.org/10.3390/v14051031
APA StyleZheng, Y., Song, K., Cai, K., Liu, L., Tang, D., Long, W., Zhai, B., Chen, J., Tao, Y., Zhao, Y., Liang, S., Huang, Q., Liu, Q., Zhang, Q., Chen, Y., Liu, Y., Li, H., Wang, P., Lan, K., ... Xu, K. (2022). B-Cell-Epitope-Based Fluorescent Quantum Dot Biosensors for SARS-CoV-2 Enable Highly Sensitive COVID-19 Antibody Detection. Viruses, 14(5), 1031. https://doi.org/10.3390/v14051031