Tripartite-Motif 21 (TRIM21) Deficiency Results in a Modest Loss of Herpes Simplex Virus (HSV)-1 Surveillance in the Trigeminal Ganglia Following Cornea Infection
Abstract
:1. Introduction
2. Materials and Methods
2.1. Mice and Infection
2.2. Virus and Plaque Assay
2.3. Real Time Reverse Transcriptase (RT)-Polymerase Chain Reaction (PCR)
2.4. Spectral Domain-Optical Coherence Tomography (SD-OCT)
2.5. Corneal Sensitivity
2.6. Cornea Pathology
2.7. Flow Cytometry
2.8. Statistical Analysis
3. Results
3.1. HSV-1 Infection Induces TRIM21 Expression in the Cornea
3.2. The Absence of TRIM21 Is Reflected in a Loss of Virus Surveillance in the TG, but Not in Cornea of HSV-1-Infected Mice
3.3. The Loss of TRIM21 Does Not Modify Corneal Pathology Compared to WT Mice
3.4. Visual Axis Function Is Reduced in WT and TRIM21 KO Mice Following HSV-1 Infectionn
3.5. T Cell Infiltration and Function Are Similar between WT, TRIM21+/−, and TRIM21 KO Mice
3.6. TG of TRIM21 KO Mice Display an Elevation in HSV-1 Lytic Gene Expression but Not Type I IFN or IFN-Inducible Genes Following Infection
4. Discussion
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Forward | Reverse | |
---|---|---|
IFNa4 | 5′-TTC TGC AAT GAC CTC CAT CA-3′ | 5′-GGC ACA GAG GCT GTG TTT CT-3′ |
mSTING | 5′-CCT AGC CTC GCA CGA ACT TG-3′ | 5′-CGC ACA GCC TTC CAG TAG C-3′ |
DAI | 5′-GGA AGA TCT ACC ACT CAC GTC-3′ | 5′-CCT TGT TGG CAG ATG ATG TTG-3′ |
Oas1a | 5′-CTT TGA TGT CCT GGG TCA TGT-3′ | 5′-GCT CCG TGA AGC AGG TAG AG-3′ |
ICP27 | 5’- GCA TCC TTC GTG TTT GTC AT-3’ | 5’- ACC AAG GGT CGC GTA GTC-3’ |
TK | 5′-ATA CCG ACG ATC TGC GAC CT-3′ | 5′-TTA TTG CCG TCA TAG CGC GG-3′ |
gB | 5′-TCT GCA CCA TGA CCA AGT G-3′ | 5′-TGG TGA AGG TGG TGG ATA TG-3′ |
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Berube, A.; Gmyrek, G.B.; Royer, D.J.; Carr, D.J.J. Tripartite-Motif 21 (TRIM21) Deficiency Results in a Modest Loss of Herpes Simplex Virus (HSV)-1 Surveillance in the Trigeminal Ganglia Following Cornea Infection. Viruses 2022, 14, 589. https://doi.org/10.3390/v14030589
Berube A, Gmyrek GB, Royer DJ, Carr DJJ. Tripartite-Motif 21 (TRIM21) Deficiency Results in a Modest Loss of Herpes Simplex Virus (HSV)-1 Surveillance in the Trigeminal Ganglia Following Cornea Infection. Viruses. 2022; 14(3):589. https://doi.org/10.3390/v14030589
Chicago/Turabian StyleBerube, Amanda, Grzegorz B. Gmyrek, Derek J. Royer, and Daniel J. J. Carr. 2022. "Tripartite-Motif 21 (TRIM21) Deficiency Results in a Modest Loss of Herpes Simplex Virus (HSV)-1 Surveillance in the Trigeminal Ganglia Following Cornea Infection" Viruses 14, no. 3: 589. https://doi.org/10.3390/v14030589