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Article

Identification and Full Characterisation of Two Novel Crustacean Infecting Members of the Family Nudiviridae Provides Support for Two Subfamilies

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International Centre of Excellence for Aquatic Animal Health (ICOE AAH), Centre for Environment, Fisheries and Aquaculture Science (Cefas), Barrack Road, The Nothe, Weymouth, Dorset DT4 8UB, UK
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The Roslin Institute and Royal (Dick), School of Veterinary Studies, Easter Bush Campus, The University of Edinburgh, Midlothian EH25 9RG, UK
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Department of Plants and Crops, Faculty of Bioscience Engineering, Ghent University, 9000 Ghent, Belgium
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Animal Sciences Unit, Fisheries, Flanders Research Institute for Agriculture, Fisheries and Food (ILVO), 8400 Ostend, Belgium
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School of Health and Life Science, Teesside University, Middlesbrough TS1 3BA, UK
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Laboratory of Virology, Plant Sciences Group, Wageningen University and Research, P.O. Box 16, 6700 AA Wageningen, The Netherlands
*
Author to whom correspondence should be addressed.
Academic Editor: Tae-Jin Choi
Viruses 2021, 13(9), 1694; https://doi.org/10.3390/v13091694
Received: 13 July 2021 / Revised: 20 August 2021 / Accepted: 23 August 2021 / Published: 26 August 2021
(This article belongs to the Section Animal Viruses)
Multiple enveloped viruses with rod-shaped nucleocapsids have been described, infecting the epithelial cell nuclei within the hepatopancreas tubules of crustaceans. These bacilliform viruses share the ultrastructural characteristics of nudiviruses, a specific clade of viruses infecting arthropods. Using histology, electron microscopy and high throughput sequencing, we characterise two further bacilliform viruses from aquatic hosts, the brown shrimp (Crangon crangon) and the European shore crab (Carcinus maenas). We assembled the full double stranded, circular DNA genome sequences of these viruses (~113 and 132 kbp, respectively). Comparative genomics and phylogenetic analyses confirm that both belong within the family Nudiviridae but in separate clades representing nudiviruses found in freshwater and marine environments. We show that the three thymidine kinase (tk) genes present in all sequenced nudivirus genomes, thus far, were absent in the Crangon crangon nudivirus, suggesting there are twenty-eight core genes shared by all nudiviruses. Furthermore, the phylogenetic data no longer support the subdivision of the family Nudiviridae into four genera (Alphanudivirus to Deltanudivirus), as recently adopted by the International Committee on Taxonomy of Viruses (ICTV), but rather shows two main branches of the family that are further subdivided. Our data support a recent proposal to create two subfamilies within the family Nudiviridae, each subdivided into several genera. View Full-Text
Keywords: nudivirus; Crangon crangon; Carcinus maenas; brown shrimp; shore crab; virus classification; core genes; Crangon crangon nudivirus (CcNV); Carcinus maenas nudivirus (CmNV); bacilliform virus; taxonomy nudivirus; Crangon crangon; Carcinus maenas; brown shrimp; shore crab; virus classification; core genes; Crangon crangon nudivirus (CcNV); Carcinus maenas nudivirus (CmNV); bacilliform virus; taxonomy
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MDPI and ACS Style

Bateman, K.S.; Kerr, R.; Stentiford, G.D.; Bean, T.P.; Hooper, C.; Van Eynde, B.; Delbare, D.; Bojko, J.; Christiaens, O.; Taning, C.N.T.; Smagghe, G.; van Oers, M.M.; van Aerle, R. Identification and Full Characterisation of Two Novel Crustacean Infecting Members of the Family Nudiviridae Provides Support for Two Subfamilies. Viruses 2021, 13, 1694. https://doi.org/10.3390/v13091694

AMA Style

Bateman KS, Kerr R, Stentiford GD, Bean TP, Hooper C, Van Eynde B, Delbare D, Bojko J, Christiaens O, Taning CNT, Smagghe G, van Oers MM, van Aerle R. Identification and Full Characterisation of Two Novel Crustacean Infecting Members of the Family Nudiviridae Provides Support for Two Subfamilies. Viruses. 2021; 13(9):1694. https://doi.org/10.3390/v13091694

Chicago/Turabian Style

Bateman, Kelly S., Rose Kerr, Grant D. Stentiford, Tim P. Bean, Chantelle Hooper, Benigna Van Eynde, Daan Delbare, Jamie Bojko, Olivier Christiaens, Clauvis N.T. Taning, Guy Smagghe, Monique M. van Oers, and Ronny van Aerle. 2021. "Identification and Full Characterisation of Two Novel Crustacean Infecting Members of the Family Nudiviridae Provides Support for Two Subfamilies" Viruses 13, no. 9: 1694. https://doi.org/10.3390/v13091694

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