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Viruses with U-DNA: New Avenues for Biotechnology

Department of Applied Biotechnology and Food Sciences, Budapest University of Biotechnology and Economics, 1111 Budapest, Hungary
Research Centre for Natural Sciences, Institute of Enzymology, 1117 Budapest, Hungary
Department of Bacteriology and Immunology, Medicum, Human Microbiome Research Program, Faculty of Medicine, University of Helsinki, 00014 Helsinki, Finland
Division of Clinical Microbiology, Helsinki University Hospital, HUSLAB, 00290 Helsinki, Finland
Authors to whom correspondence should be addressed.
Academic Editor: Joana Azeredo
Viruses 2021, 13(5), 875;
Received: 22 February 2021 / Revised: 5 May 2021 / Accepted: 6 May 2021 / Published: 10 May 2021
Deoxyuridine in DNA has recently been in the focus of research due to its intriguing roles in several physiological and pathophysiological situations. Although not an orthodox DNA base, uracil may appear in DNA via either cytosine deamination or thymine-replacing incorporations. Since these alterations may induce mutation or may perturb DNA–protein interactions, free living organisms from bacteria to human contain several pathways to counteract uracilation. These efficient and highly specific repair routes uracil-directed excision repair initiated by representative of uracil-DNA glycosylase families. Interestingly, some bacteriophages exist with thymine-lacking uracil-DNA genome. A detailed understanding of the strategy by which such phages can replicate in bacteria where an efficient repair pathway functions for uracil-excision from DNA is expected to reveal novel inhibitors that can also be used for biotechnological applications. Here, we also review the several potential biotechnological applications already implemented based on inhibitors of uracil-excision repair, such as Crispr-base-editing and detection of nascent uracil distribution pattern in complex genomes. View Full-Text
Keywords: phages; uracil-DNA; biotechnology phages; uracil-DNA; biotechnology
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MDPI and ACS Style

Nagy, K.K.; Skurnik, M.; Vértessy, B.G. Viruses with U-DNA: New Avenues for Biotechnology. Viruses 2021, 13, 875.

AMA Style

Nagy KK, Skurnik M, Vértessy BG. Viruses with U-DNA: New Avenues for Biotechnology. Viruses. 2021; 13(5):875.

Chicago/Turabian Style

Nagy, Kinga K., Mikael Skurnik, and Beáta G. Vértessy 2021. "Viruses with U-DNA: New Avenues for Biotechnology" Viruses 13, no. 5: 875.

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