Phenotypic Divergence of P Proteins of Australian Bat Lyssavirus Lineages Circulating in Microbats and Flying Foxes
Abstract
1. Introduction
2. Materials and Methods
2.1. DNA Constructs, Cell Culture, and Transfections
2.2. Reporter Gene Assays
2.3. Co-Immunoprecipitation (IP)
2.4. Confocal Laser Scanning Microscopy (CLSM)
2.5. Statistical Analysis
3. Results and Discussion
3.1. Antagonism of IFN Signaling Differs between ABLVi and ABLVf P Proteins
3.2. Nuclear Trafficking Differs between ABLVf and ABLVi P Protein
3.3. Differing Nuclear Trafficking and IFN Antagonism Depend on Distinct Regions of ABLV P Protein
3.4. Differing Antagonistic Functions of ABLV P Protein Are Due to Altered Interaction with STAT1
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Function | P Protein | |||||
---|---|---|---|---|---|---|
ABLVf | ABLVi | i/i/f | f/i/i | i/f/f | i/f/i | |
Nuclear Import | +++ | + | + | + | +++ | ++ |
IFN antagonism | +++ | + | +++ | ++ | +++ | ++ |
STAT1-binding | +++ | + | +++ | + | +++ | + |
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Deffrasnes, C.; Luo, M.-X.; Wiltzer-Bach, L.; David, C.T.; Lieu, K.G.; Wang, L.-F.; Jans, D.A.; Marsh, G.A.; Moseley, G.W. Phenotypic Divergence of P Proteins of Australian Bat Lyssavirus Lineages Circulating in Microbats and Flying Foxes. Viruses 2021, 13, 831. https://doi.org/10.3390/v13050831
Deffrasnes C, Luo M-X, Wiltzer-Bach L, David CT, Lieu KG, Wang L-F, Jans DA, Marsh GA, Moseley GW. Phenotypic Divergence of P Proteins of Australian Bat Lyssavirus Lineages Circulating in Microbats and Flying Foxes. Viruses. 2021; 13(5):831. https://doi.org/10.3390/v13050831
Chicago/Turabian StyleDeffrasnes, Celine, Meng-Xiao Luo, Linda Wiltzer-Bach, Cassandra T. David, Kim G. Lieu, Lin-Fa Wang, David A. Jans, Glenn A. Marsh, and Gregory W. Moseley. 2021. "Phenotypic Divergence of P Proteins of Australian Bat Lyssavirus Lineages Circulating in Microbats and Flying Foxes" Viruses 13, no. 5: 831. https://doi.org/10.3390/v13050831
APA StyleDeffrasnes, C., Luo, M.-X., Wiltzer-Bach, L., David, C. T., Lieu, K. G., Wang, L.-F., Jans, D. A., Marsh, G. A., & Moseley, G. W. (2021). Phenotypic Divergence of P Proteins of Australian Bat Lyssavirus Lineages Circulating in Microbats and Flying Foxes. Viruses, 13(5), 831. https://doi.org/10.3390/v13050831