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Open AccessArticle

Isolation and Characterisation of Bacteriophages with Activity against Invasive Non-Typhoidal Salmonella Causing Bloodstream Infection in Malawi

1
Clinical Infection, Microbiology and Immunology, Institute of Infection, Veterinary and Ecological Sciences, University of Liverpool, Liverpool L69 7ZB, UK
2
Biomedical Electron Microscopy Facility, University of Liverpool, Liverpool L69 7ZB, UK
3
Department of Clinical Sciences, Liverpool School of Tropical Medicine, Liverpool L3 5QA, UK
4
Malawi Liverpool Wellcome Trust Clinical Research Programme, The College of Medicine, University of Malawi, Blantyre 3, Malawi
5
Department of Biosciences, Durham University, Durham DH1 3LE, UK
*
Author to whom correspondence should be addressed.
Current affiliation: NIHR HPRU in Gastrointestinal Infections at University of Liverpool, Liverpool L69 7ZB, UK.
Academic Editor: Dann Turner
Viruses 2021, 13(3), 478; https://doi.org/10.3390/v13030478
Received: 21 February 2021 / Revised: 10 March 2021 / Accepted: 11 March 2021 / Published: 15 March 2021
(This article belongs to the Section Bacterial Viruses)
In recent years, novel lineages of invasive non-typhoidal Salmonella (iNTS) serovars Typhimurium and Enteritidis have been identified in patients with bloodstream infection in Sub-Saharan Africa. Here, we isolated and characterised 32 phages capable of infecting S. Typhimurium and S. Enteritidis, from water sources in Malawi and the UK. The phages were classified in three major phylogenetic clusters that were geographically distributed. In terms of host range, Cluster 1 phages were able to infect all bacterial hosts tested, whereas Clusters 2 and 3 had a more restricted profile. Cluster 3 contained two sub-clusters, and 3.b contained the most novel isolates. This study represents the first exploration of the potential for phages to target the lineages of Salmonella that are responsible for bloodstream infections in Sub-Saharan Africa. View Full-Text
Keywords: Enteritidis; Typhimurium; Malawi; environmental phage Enteritidis; Typhimurium; Malawi; environmental phage
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MDPI and ACS Style

Rodwell, E.V.; Wenner, N.; Pulford, C.V.; Cai, Y.; Bowers-Barnard, A.; Beckett, A.; Rigby, J.; Picton, D.M.; Blower, T.R.; Feasey, N.A.; Hinton, J.C.D.; Perez-Sepulveda, B.M. Isolation and Characterisation of Bacteriophages with Activity against Invasive Non-Typhoidal Salmonella Causing Bloodstream Infection in Malawi. Viruses 2021, 13, 478. https://doi.org/10.3390/v13030478

AMA Style

Rodwell EV, Wenner N, Pulford CV, Cai Y, Bowers-Barnard A, Beckett A, Rigby J, Picton DM, Blower TR, Feasey NA, Hinton JCD, Perez-Sepulveda BM. Isolation and Characterisation of Bacteriophages with Activity against Invasive Non-Typhoidal Salmonella Causing Bloodstream Infection in Malawi. Viruses. 2021; 13(3):478. https://doi.org/10.3390/v13030478

Chicago/Turabian Style

Rodwell, Ella V.; Wenner, Nicolas; Pulford, Caisey V.; Cai, Yueyi; Bowers-Barnard, Arthur; Beckett, Alison; Rigby, Jonathan; Picton, David M.; Blower, Tim R.; Feasey, Nicholas A.; Hinton, Jay C.D.; Perez-Sepulveda, Blanca M. 2021. "Isolation and Characterisation of Bacteriophages with Activity against Invasive Non-Typhoidal Salmonella Causing Bloodstream Infection in Malawi" Viruses 13, no. 3: 478. https://doi.org/10.3390/v13030478

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