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Open AccessArticle

Detection of Genotype-Specific Antibody Responses to Glycoproteins B and H in Primary and Non-Primary Human Cytomegalovirus Infections by Peptide-Based ELISA

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Laboratorio Genetica—Trapiantologia e Malattie Cardiovascolari, Fondazione IRCCS Policlinico San Matteo, 27100 Pavia, Italy
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Microbiologia e Virologia, Fondazione IRCCS Policlinico San Matteo, 27100 Pavia, Italy
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Laboratorio Biochimica-Biotecnologie e Diagnostica Avanzata, Fondazione IRCCS Policlinico San Matteo, 27100 Pavia, Italy
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MRC-University of Glasgow Centre for Virus Research, Glasgow G61 1AF, UK
*
Author to whom correspondence should be addressed.
Academic Editors: Thomas Stamminger and Soren Gantt
Viruses 2021, 13(3), 399; https://doi.org/10.3390/v13030399
Received: 13 January 2021 / Revised: 19 February 2021 / Accepted: 25 February 2021 / Published: 3 March 2021
(This article belongs to the Special Issue Cytomegalovirus Immunity)
Background: Strain-specific antibodies to human cytomegalovirus (HCMV) glycoproteins B and H (gB and gH) have been proposed as a potential diagnostic tool for identifying reinfection. We investigated genotype-specific IgG antibody responses in parallel with defining the gB and gH genotypes of the infecting viral strains. Methods: Subjects with primary (n = 20) or non-primary (n = 25) HCMV infection were studied. The seven gB (gB1-7) and two gH (gH1-2) genotypes were determined by real-time PCR and whole viral genome sequencing, and genotype-specific IgG antibodies were measured by a peptide-based enzyme-linked immunosorbent assay (ELISA). Results: Among subjects with primary infection, 73% (n = 8) infected by gB1-HCMV and 63% (n = 5) infected by gB2/3-HCMV had genotype-specific IgG antibodies to gB (gB2 and gB3 are similar in the region tested). Peptides from the rarer gB4-gB7 genotypes had nonspecific antibody responses. All subjects infected by gH1-HCMV and 86% (n = 6) infected by gH2-HCMV developed genotype-specific responses. Among women with non-primary infection, gB and gH genotype-specific IgG antibodies were detected in 40% (n = 10) and 80% (n = 20) of subjects, respectively. Conclusions: Peptide-based ELISA is capable of detecting primary genotype-specific IgG responses to HCMV gB and gH, and could be adopted for identifying reinfections. However, about half of the subjects did not have genotype-specific IgG antibodies to gB. View Full-Text
Keywords: human cytomegalovirus; glycoprotein B; glycoprotein H; genotype; antibody; primary infection; non-primary infection human cytomegalovirus; glycoprotein B; glycoprotein H; genotype; antibody; primary infection; non-primary infection
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MDPI and ACS Style

Zavaglio, F.; Fiorina, L.; Suárez, N.M.; Fornara, C.; De Cicco, M.; Cirasola, D.; Davison, A.J.; Gerna, G.; Lilleri, D. Detection of Genotype-Specific Antibody Responses to Glycoproteins B and H in Primary and Non-Primary Human Cytomegalovirus Infections by Peptide-Based ELISA. Viruses 2021, 13, 399. https://doi.org/10.3390/v13030399

AMA Style

Zavaglio F, Fiorina L, Suárez NM, Fornara C, De Cicco M, Cirasola D, Davison AJ, Gerna G, Lilleri D. Detection of Genotype-Specific Antibody Responses to Glycoproteins B and H in Primary and Non-Primary Human Cytomegalovirus Infections by Peptide-Based ELISA. Viruses. 2021; 13(3):399. https://doi.org/10.3390/v13030399

Chicago/Turabian Style

Zavaglio, Federica; Fiorina, Loretta; Suárez, Nicolás M.; Fornara, Chiara; De Cicco, Marica; Cirasola, Daniela; Davison, Andrew J.; Gerna, Giuseppe; Lilleri, Daniele. 2021. "Detection of Genotype-Specific Antibody Responses to Glycoproteins B and H in Primary and Non-Primary Human Cytomegalovirus Infections by Peptide-Based ELISA" Viruses 13, no. 3: 399. https://doi.org/10.3390/v13030399

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