TMPRSS11D and TMPRSS13 Activate the SARS-CoV-2 Spike Protein
Abstract
1. Introduction
2. Materials and Methods
2.1. Cells and Viruses
2.2. Plasmids and Transfection
2.3. Generation of Vero E6 Cells Stably Expressing TTSPs
2.4. Immunoblotting
2.5. Virus Entry Assay
2.6. Multi-Cycle Replication Assay
2.7. Indirect Immunofluorescence Assay (IFA)
2.8. Statistical Analysis
3. Results
3.1. Evaluation of the Effects of TTSPs on the Entry of SARS-CoV-1 and SARS-CoV-2 Using 293T-ACE2 Cells
3.2. TMPRSS11D and 13 Facilitate SARS-CoV-2 Replication
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Kishimoto, M.; Uemura, K.; Sanaki, T.; Sato, A.; Hall, W.W.; Kariwa, H.; Orba, Y.; Sawa, H.; Sasaki, M. TMPRSS11D and TMPRSS13 Activate the SARS-CoV-2 Spike Protein. Viruses 2021, 13, 384. https://doi.org/10.3390/v13030384
Kishimoto M, Uemura K, Sanaki T, Sato A, Hall WW, Kariwa H, Orba Y, Sawa H, Sasaki M. TMPRSS11D and TMPRSS13 Activate the SARS-CoV-2 Spike Protein. Viruses. 2021; 13(3):384. https://doi.org/10.3390/v13030384
Chicago/Turabian StyleKishimoto, Mai, Kentaro Uemura, Takao Sanaki, Akihiko Sato, William W. Hall, Hiroaki Kariwa, Yasuko Orba, Hirofumi Sawa, and Michihito Sasaki. 2021. "TMPRSS11D and TMPRSS13 Activate the SARS-CoV-2 Spike Protein" Viruses 13, no. 3: 384. https://doi.org/10.3390/v13030384
APA StyleKishimoto, M., Uemura, K., Sanaki, T., Sato, A., Hall, W. W., Kariwa, H., Orba, Y., Sawa, H., & Sasaki, M. (2021). TMPRSS11D and TMPRSS13 Activate the SARS-CoV-2 Spike Protein. Viruses, 13(3), 384. https://doi.org/10.3390/v13030384