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Transcriptome Sequencing of the Spleen Reveals Antiviral Response Genes in Chickens Infected with CAstV

1
Department of Poultry Diseases, National Veterinary Research Institute, 24-100 Puławy, Poland
2
Department of Plant Physiology, Genetics and Biotechnology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, 10-719 Olsztyn, Poland
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Department of Tropical Parasitology, Institute of Maritime and Tropical Medicine, Medical University of Gdansk, 81-519 Gdynia, Poland
4
Department of Clinical Pathomorphology, The Medical University of Lublin, 20-090 Lublin, Poland
*
Author to whom correspondence should be addressed.
Academic Editors: Chao-Nan Lin and Peck Toung Ooi
Viruses 2021, 13(12), 2374; https://doi.org/10.3390/v13122374
Received: 11 October 2021 / Revised: 19 November 2021 / Accepted: 22 November 2021 / Published: 26 November 2021
(This article belongs to the Topic Veterinary Infectious Diseases)
Astrovirus infections pose a significant problem in the poultry industry, leading to multiple adverse effects such as a decreased egg production, breeding disorders, poor weight gain, and even increased mortality. The commonly observed chicken astrovirus (CAstV) was recently reported to be responsible for the “white chicks syndrome” associated with an increased embryo/chick mortality. CAstV-mediated pathogenesis in chickens occurs due to complex interactions between the infectious pathogen and the immune system. Many aspects of CAstV–chicken interactions remain unclear, and there is no information available regarding possible changes in gene expression in the chicken spleen in response to CAstV infection. We aim to investigate changes in gene expression triggered by CAstV infection. Ten 21-day-old SPF White Leghorn chickens were divided into two groups of five birds each. One group was inoculated with CAstV, and the other used as the negative control. At 4 days post infection, spleen samples were collected and immediately frozen at −70 °C for RNA isolation. We analyzed the isolated RNA, using RNA-seq to generate transcriptional profiles of the chickens’ spleens and identify differentially expressed genes (DEGs). The RNA-seq findings were verified by quantitative reverse-transcription PCR (qRT-PCR). A total of 31,959 genes was identified in response to CAstV infection. Eventually, 45 DEGs (p-value < 0.05; log2 fold change > 1) were recognized in the spleen after CAstV infection (26 upregulated DEGs and 19 downregulated DEGs). qRT-PCR performed on four genes (IFIT5, OASL, RASD1, and DDX60) confirmed the RNA-seq results. The most differentially expressed genes encode putative IFN-induced CAstV restriction factors. Most DEGs were associated with the RIG-I-like signaling pathway or more generally with an innate antiviral response (upregulated: BLEC3, CMPK2, IFIT5, OASL, DDX60, and IFI6; downregulated: SPIK5, SELENOP, HSPA2, TMEM158, RASD1, and YWHAB). The study provides a global analysis of host transcriptional changes that occur during CAstV infection in vivo and proves that, in the spleen, CAstV infection in chickens predominantly affects the cell cycle and immune signaling. View Full-Text
Keywords: chicken astrovirus; white chicks syndrome; RNA-seq; molecular pathogenesis; differentially expressed genes; spleen transcriptome chicken astrovirus; white chicks syndrome; RNA-seq; molecular pathogenesis; differentially expressed genes; spleen transcriptome
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MDPI and ACS Style

Sajewicz-Krukowska, J.; Jastrzębski, J.P.; Grzybek, M.; Domańska-Blicharz, K.; Tarasiuk, K.; Marzec-Kotarska, B. Transcriptome Sequencing of the Spleen Reveals Antiviral Response Genes in Chickens Infected with CAstV. Viruses 2021, 13, 2374. https://doi.org/10.3390/v13122374

AMA Style

Sajewicz-Krukowska J, Jastrzębski JP, Grzybek M, Domańska-Blicharz K, Tarasiuk K, Marzec-Kotarska B. Transcriptome Sequencing of the Spleen Reveals Antiviral Response Genes in Chickens Infected with CAstV. Viruses. 2021; 13(12):2374. https://doi.org/10.3390/v13122374

Chicago/Turabian Style

Sajewicz-Krukowska, Joanna, Jan P. Jastrzębski, Maciej Grzybek, Katarzyna Domańska-Blicharz, Karolina Tarasiuk, and Barbara Marzec-Kotarska. 2021. "Transcriptome Sequencing of the Spleen Reveals Antiviral Response Genes in Chickens Infected with CAstV" Viruses 13, no. 12: 2374. https://doi.org/10.3390/v13122374

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